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ANAMMOX Based Technology for Nitrogen Removal from Wastewater: Linking Microbial Interactions, Ecological Mechanisms, and Engineering Optimization, 2nd Edition

A special issue of Water (ISSN 2073-4441). This special issue belongs to the section "Wastewater Treatment and Reuse".

Deadline for manuscript submissions: 30 September 2026 | Viewed by 963

Special Issue Editor


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Guest Editor
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
Interests: anammox; partial nitrification; greenhouse gas; quorum sensing; bacterial community; free nitrous acid
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Special Issue Information

Dear Colleagues,

Nitrogen pollution remains a critical global environmental challenge, driving eutrophication in aquatic ecosystems and posing significant public health risks yet, while traditional biological nitrogen removal processes are established, they are often energy-intensive and carry a heavy carbon footprint. In this context, Anaerobic Ammonium Oxidation (ANAMMOX) has emerged as a paradigm-shifting technology for sustainable wastewater treatment. By converting ammonium and nitrite directly into dinitrogen gas, ANAMMOX bypasses the substantial aeration and external carbon requirements of conventional nitrification–denitrification, offering a distinct advantage in energy neutrality and operational cost reduction.

This Special Issue aims to bridge the gap between fundamental microbiology and practical engineering applications. We seek to unravel the complexities of the ANAMMOX process by linking microbial interactions (e.g., quorum sensing and syntrophy) and ecological mechanisms (e.g., community assembly and niche differentiation) with engineering optimization. The Special Issue will explore how these biological and ecological factors dictate system stability and performance, and how this understanding can be translated into robust reactor designs and control strategies. Furthermore, we will address the broader environmental implications, specifically the role of ANAMMOX in achieving carbon-neutral wastewater treatment and resource recovery.

Nitrogen pollution in wastewater has become a significant environmental challenge worldwide, leading to eutrophication of water bodies and potential health risks. Traditional biological nitrogen removal processes, while effective, often require high energy input and have a large carbon footprint. In recent years, the Anaerobic Ammonium Oxidation (ANAMMOX) process has emerged as a promising and sustainable alternative for nitrogen removal from wastewater. ANAMMOX bacteria can convert ammonium directly to nitrogen gas using nitrite as an electron acceptor, bypassing the need for aeration and external carbon sources. This innovative biotechnology offers substantial benefits in terms of energy savings, reduced greenhouse gas emissions, and lower operational costs compared to conventional nitrification-denitrification processes.

This Special Issue focuses on the latest advancements in ANAMMOX biotechnology for nitrogen removal from wastewater. It aims to explore both the fundamental science and engineering applications of the ANAMMOX process, covering key topics such as partial nitrification, greenhouse gas emissions, quorum sensing, bacterial community dynamics. The Special Issue will delve into how these factors influence the performance and stability of ANAMMOX systems, and how to optimize the process for improved nitrogen removal efficiency. It will also address the broader implications of ANAMMOX technology, including its role in achieving energy-efficient and environmentally sustainable wastewater treatment, and will explore how ANAMMOX contributes to reducing the carbon footprint of wastewater treatment plants and its potential in resource recovery.

This Special Issue invites the submission of original research articles and critical reviews that advance the state-of-the-art in ANAMMOX technology. We particularly welcome studies that integrate microbial insights with process engineering. Potential topics include, but are not limited to:

  1. Microbial Ecology and Interactions: Bacterial community dynamics, quorum sensing mechanisms, and symbiotic interactions within ANAMMOX granular sludge or biofilms.
  2. Process Mechanisms: Insights into partial nitrification–anammox (PN/A), metabolic pathways, and inhibition kinetics.
  3. Engineering Optimization: Advanced reactor configurations, real-time control strategies, and scalability of ANAMMOX systems.
  4. Environmental Impact: Assessment and mitigation of greenhouse gas emissions in ANAMMOX-based processes.
  5. Application and Integration: Treatment of mainstream and varying industrial wastewaters.

Dr. Cancan Jiang
Guest Editor

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Keywords

  • anammox
  • partial nitrification
  • microbial interactions
  • greenhouse gas
  • quorum sensing
  • bacterial community
  • carbon neutrality

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Related Special Issue

Published Papers (2 papers)

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Research

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24 pages, 10507 KB  
Article
Treatment of Liquor Wastewater by an Integration of Anaerobic Digestion, Partial Nitrification/Anammox, and Fenton Process: Performance and Microbial Analysis
by Jing Zhang, Hui Wang, Yaxuan Xiao, Junmei Wu, Qi Liu, Bi Chen, Hongyu Wang and Qiaohong Zhou
Water 2026, 18(10), 1179; https://doi.org/10.3390/w18101179 - 13 May 2026
Viewed by 148
Abstract
This study designed an integration of anaerobic digestion, partial nitrification/Anammox (PN/A), and Fenton process to efficiently treat high-concentration organic liquor wastewater (HCLW). Results indicated that when the diluted ten-fold mixture of boiler bottom water and cellar bottom water with the ratio of 5:1 [...] Read more.
This study designed an integration of anaerobic digestion, partial nitrification/Anammox (PN/A), and Fenton process to efficiently treat high-concentration organic liquor wastewater (HCLW). Results indicated that when the diluted ten-fold mixture of boiler bottom water and cellar bottom water with the ratio of 5:1 was used as influent, the average concentrations of COD, TN, NH4+-N, NO2-N, and NO3-N in effluent of biological treatment for this process were 180.00, 12.64, 1.74, 0.13, and 2.45 mg/L, respectively. To meet the requirement for direct discharge of HCLW, Fenton oxidation with 600 mg H2O2/L and 300 mg Fe2+/L was used to further reduce the COD concentration. Three-dimensional fluorescence spectra analysis revealed that the process effectively altered the organic molecular structure and degraded some large molecular proteins. Microbial community analysis showed that Methanobacterium (20.98% → 31.52%) and Methanosaeta (9.70% → 19.34%) in AD, Azoarcus (no detected → 10.49%) and Nitrosomonas (1.68% → 6.16%) in PN, and Candidatus_Brocadia (18.80% → 20.31%) and Ignavibacterium (no detected → 5.11%) in Anammox were dominant in this system. This study provided a pioneering industrial solution for the efficient and stable treatment of HCLW. Full article

Review

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17 pages, 2142 KB  
Review
The Impact of Climate Change on Nitrogen Migration and Transformation in Inland Water Bodies: A Bibliometric Landscape Analysis
by Danhua Wang, Cancan Jiang, Xu Wang, Huijuan Feng and Hongjie Gao
Water 2026, 18(5), 646; https://doi.org/10.3390/w18050646 - 9 Mar 2026
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Abstract
Based on a bibliometric analysis of 2680 publications (1962–2024), this study elucidates the knowledge structure and intellectual evolution of research on climate change-driven nitrogen migration and transformation in inland waters, a critical issue for water security and global climate stability. The field has [...] Read more.
Based on a bibliometric analysis of 2680 publications (1962–2024), this study elucidates the knowledge structure and intellectual evolution of research on climate change-driven nitrogen migration and transformation in inland waters, a critical issue for water security and global climate stability. The field has experienced accelerated growth since 2016, led by the United States and China. Analysis reveals a research framework centered on climate change, nitrogen, and water quality, interconnected with processes like eutrophication and denitrification. The intellectual focus has evolved from early investigations into fundamental chemical mechanisms towards a contemporary emphasis on human–climate interactions (e.g., land use), model-based predictions, and regional management solutions for nonpoint source pollution. A key finding is the bidirectional climate–nitrogen feedback, where climate alters nitrogen pathways and transformations, which in turn release greenhouse gases. The findings underscore a pivotal shift from theoretical understanding to applied, solution-oriented research. Future work must prioritize integrated multi-technique approaches, cross-ecosystem comparisons, and data-driven modeling to advance predictive capabilities and support effective nitrogen management in inland waters under a changing climate. Full article
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