Submit to Special Issue Submit Abstract to Special Issue Review for Viruses Propose a Special Issue

Journal Browser

► Journal Browser

Special Issue "Pathogenesis of Viral Infections: Implications in the Development of Vaccines and Diagnostic Tools 2.0"

Print Special Issue Flyer
Special Issue Editors
Special Issue Information
Keywords
Published Papers

A special issue of Viruses (ISSN 1999-4915). This special issue belongs to the section "Animal Viruses".

Deadline for manuscript submissions: 31 December 2023 | Viewed by 3050

Share This Special Issue

Special Issue Editors

Dr. Llilianne Ganges

E-Mail Website
Guest Editor

OIE Reference Laboratory for Classical Swine Fever, Institute of Agrifood Research and Technology (IRTA), Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona, 08193 Barcelona, Spain
Interests: virology; viral pathogenesis and immunology; innate and adaptive immunity; virus host interaction; viral evolution; vaccine and diagnostic tools design; viruses in animal health
Special Issues, Collections and Topics in MDPI journals

Prof. Dr. Jishu Shi

E-Mail Website
Guest Editor

College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA
Interests: African swine fever (ASF); porcine reproductive and respiratory syndrome (PRRS); classical swine fever virus (CSF); animal vaccine design and evaluation; adjuvant development; diagnostic tool development and evaluation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue focuses on gathering articles that show the most relevant aspects of viral pathogenesis that constitute novel and strategic targets for the development of vaccines and diagnostic methods of relevant diseases that affect animal health. We will focus on the mechanisms of viral persistence, specifically virus–host interactions, immunology, and immunopathology. The prevention and rapid detection capacity of possible new virus emergencies that may affect animal and human health is one of the great challenges that we must face in the near future. The development of rapid and cost-effective diagnostic methods, as well as new vaccine strategies, will be key tools to overcome these challenges. Fundamental and applied research in immunology and vaccinology will also be collected in this issue.

Dr. Llilianne Ganges
Prof. Dr. Jishu Shi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

innate and adaptive immunity
vaccine development
oral vaccination
virus diagnostic
emerging virus
next-generation sequencing
virus variant detection
viruses in animal health

Published Papers (3 papers)

Download All Papers

Research

Open AccessArticle

Generation and Efficacy of Two Chimeric Viruses Derived from GPE⁻ Vaccine Strain as Classical Swine Fever Vaccine Candidates

and

Viruses 2023, 15(7), 1587; https://doi.org/10.3390/v15071587 - 20 Jul 2023

Viewed by 785

Abstract

A previous study proved that vGPE⁻ mainly maintains the properties of classical swine fever (CSF) virus, which is comparable to the GPE⁻ vaccine seed and is a potentially valuable backbone for developing a CSF marker vaccine. Chimeric viruses were constructed based on an infectious cDNA clone derived from the live attenuated GPE⁻ vaccine strain as novel CSF vaccine candidates that potentially meet the concept of differentiating infected from vaccinated animals (DIVA) by substituting the glycoprotein E^rns of the GPE⁻ vaccine strain with the corresponding region of non-CSF pestiviruses, either pronghorn antelope pestivirus (PAPeV) or Phocoena pestivirus (PhoPeV). High viral growth and genetic stability after serial passages of the chimeric viruses, namely vGPE⁻/PAPeV E^rns and vGPE⁻/PhoPeV E^rns, were confirmed in vitro. In vivo investigation revealed that two chimeric viruses had comparable immunogenicity and safety profiles to the vGPE⁻ vaccine strain. Vaccination at a dose of 10^4.0 TCID₅₀ with either vGPE⁻/PAPeV E^rns or vGPE⁻/PhoPeV E^rns conferred complete protection for pigs against the CSF virus challenge in the early stage of immunization. In conclusion, the characteristics of vGPE⁻/PAPeV E^rns and vGPE⁻/PhoPeV E^rns affirmed their properties, as the vGPE⁻ vaccine strain, positioning them as ideal candidates for future development of a CSF marker vaccine. Full article

(This article belongs to the Special Issue Pathogenesis of Viral Infections: Implications in the Development of Vaccines and Diagnostic Tools 2.0)

► Show Figures

Figure 1

Open AccessEditor’s ChoiceArticle

A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs

Francisco Jesus Castañeda-Montes

José Luis Cerriteño-Sánchez

María Azucena Castañeda-Montes

Julieta Sandra Cuevas-Romero

and

Susana Mendoza-Elvira

Viruses 2023, 15(5), 1049; https://doi.org/10.3390/v15051049 - 25 Apr 2023

Cited by 1 | Viewed by 1080

Abstract

Porcine deltacoronavirus (PDCoV) is an emergent swine coronavirus which infects cells from the small intestine and induces watery diarrhea, vomiting and dehydration, causing mortality in piglets (>40%). The aim of this study was to evaluate the antigenicity and immunogenicity of the recombinant membrane protein (M) of PDCoV (rM-PDCoV), which was developed from a synthetic gene obtained after an in silico analysis with a group of 138 GenBank sequences. A 3D model and phylogenetic analysis confirmed the highly conserved M protein structure. Therefore, the synthetic gene was successfully cloned in a pETSUMO vector and transformed in E. coli BL21 (DE3). The rM-PDCoV was confirmed by SDS-PAGE and Western blot with ~37.7 kDa. The rM-PDCoV immunogenicity was evaluated in immunized (BLAB/c) mice and iELISA. The data showed increased antibodies from 7 days until 28 days (p < 0.001). The rM-PDCoV antigenicity was analyzed using pig sera samples from three states located in “El Bajío” Mexico and positive sera were determined. Our results show that PDCoV has continued circulating on pig farms in Mexico since the first report in 2019; therefore, the impact of PDCoV on the swine industry could be higher than reported in other studies. Full article

(This article belongs to the Special Issue Pathogenesis of Viral Infections: Implications in the Development of Vaccines and Diagnostic Tools 2.0)

► Show Figures

Graphical abstract

Open AccessArticle

Development of Porcine Monoclonal Antibodies with In Vitro Neutralizing Activity against Classical Swine Fever Virus from C-Strain E2-Specific Single B Cells

and

Viruses 2023, 15(4), 863; https://doi.org/10.3390/v15040863 - 28 Mar 2023

Cited by 1 | Viewed by 793

Abstract

Neutralizing antibodies (nAbs) can be used before or after infection to prevent or treat viral diseases. However, there are few efficacious nAbs against classical swine fever virus (CSFV) that have been produced, especially the porcine-originated nAbs. In this study, we generated three porcine monoclonal antibodies (mAbs) with in vitro neutralizing activity against CSFV, aiming to facilitate the development of passive antibody vaccines or antiviral drugs against CSFV that offer the advantages of stability and low immunogenicity. Pigs were immunized with the C-strain E2 (CE2) subunit vaccine, KNB-E2. At 42 days post vaccination (DPV), CE2-specific single B cells were isolated via fluorescent-activated cell sorting (FACS) baited by Alexa Fluor™ 647-labeled CE2 (positive), goat anti-porcine IgG (H + L)-FITC antibody (positive), PE mouse anti-pig CD3ε (negative) and PE mouse anti-pig CD8a (negative). The full coding region of IgG heavy (H) chains and light (L) chains was amplified by reverse transcription-polymerase chain reaction (RT-PCR). Overall, we obtained 3 IgG H chains, 9 kappa L chains and 36 lambda L chains, which include three paired chains (two H + κ and one H + λ). CE2-specific mAbs were successfully expressed in 293T cells with the three paired chains. The mAbs exhibit potent neutralizing activity against CSFVs. They can protect ST cells from infections in vitro with potent IC₅₀ values from 14.43 µg/mL to 25.98 µg/mL for the CSFV C-strain, and 27.66 µg/mL to 42.61 µg/mL for the CSFV Alfort strain. This study is the first report to describe the amplification of whole-porcine IgG genes from single B cells of KNB-E2-vaccinated pig. The method is versatile, sensitive, and reliable. The generated natural porcine nAbs can be used to develop long-acting and low-immunogenicity passive antibody vaccine or anti-CSFV agents for CSF control and prevention. Full article

(This article belongs to the Special Issue Pathogenesis of Viral Infections: Implications in the Development of Vaccines and Diagnostic Tools 2.0)

► Show Figures

Figure 1

Journal Menu

Journal Browser

Special Issue "Pathogenesis of Viral Infections: Implications in the Development of Vaccines and Diagnostic Tools 2.0"

Share This Special Issue

Special Issue Editors

Special Issue Information

Keywords

Published Papers (3 papers)

Research

Further Information

Guidelines

MDPI Initiatives

Follow MDPI