African Swine Fever Vaccine Candidate ASFV-G-ΔI177L Produced in the Swine Macrophage-Derived Cell Line IPKM Remains Genetically Stable and Protective against Homologous Virulent Challenge
Abstract
:1. Introduction
2. Materials and Methods
2.1. Viruses and Cells
2.2. Sequencing and Analysis of the ASFV-G-∆I177L Genome
2.3. Differential Detection of ASFV-G Genome in Challenge Animals
2.4. Detection of ASFV Specific Antibodies
2.5. Evaluation of ASFV-G-ΔI177Lp10 Efficacy in Domestic Pigs
3. Results and Discussion
3.1. Successive Passages of ASFV-G-ΔI177L in IPKM Cells
3.2. Genomic Modifications of ASFV-G-ΔI177L during Passages in IPKM Cells
3.3. Assessment of Kinetic Replication of ASFV-G-ΔI177L in IPKM Cells
3.4. Assessment of ASFV-G-ΔI177Lp10 Replication in Domestic Pigs
3.5. Assessment of the Efficacy of ASFV-G-ΔI177Lp10 to Protect Domestic Pigs against the Challenge with the Virulent Parental ASFV-G
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Borca, M.V.; Rai, A.; Espinoza, N.; Ramirez-Medina, E.; Spinard, E.; Velazquez-Salinas, L.; Valladares, A.; Silva, E.; Burton, L.; Meyers, A.; et al. African Swine Fever Vaccine Candidate ASFV-G-ΔI177L Produced in the Swine Macrophage-Derived Cell Line IPKM Remains Genetically Stable and Protective against Homologous Virulent Challenge. Viruses 2023, 15, 2064. https://doi.org/10.3390/v15102064
Borca MV, Rai A, Espinoza N, Ramirez-Medina E, Spinard E, Velazquez-Salinas L, Valladares A, Silva E, Burton L, Meyers A, et al. African Swine Fever Vaccine Candidate ASFV-G-ΔI177L Produced in the Swine Macrophage-Derived Cell Line IPKM Remains Genetically Stable and Protective against Homologous Virulent Challenge. Viruses. 2023; 15(10):2064. https://doi.org/10.3390/v15102064
Chicago/Turabian StyleBorca, Manuel V., Ayushi Rai, Nallely Espinoza, Elizabeth Ramirez-Medina, Edward Spinard, Lauro Velazquez-Salinas, Alyssa Valladares, Ediane Silva, Leeanna Burton, Amanda Meyers, and et al. 2023. "African Swine Fever Vaccine Candidate ASFV-G-ΔI177L Produced in the Swine Macrophage-Derived Cell Line IPKM Remains Genetically Stable and Protective against Homologous Virulent Challenge" Viruses 15, no. 10: 2064. https://doi.org/10.3390/v15102064
APA StyleBorca, M. V., Rai, A., Espinoza, N., Ramirez-Medina, E., Spinard, E., Velazquez-Salinas, L., Valladares, A., Silva, E., Burton, L., Meyers, A., Gay, C. G., & Gladue, D. P. (2023). African Swine Fever Vaccine Candidate ASFV-G-ΔI177L Produced in the Swine Macrophage-Derived Cell Line IPKM Remains Genetically Stable and Protective against Homologous Virulent Challenge. Viruses, 15(10), 2064. https://doi.org/10.3390/v15102064