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Veterinary Sciences
Special Issues
Latest Research on the Swine Pseudorabies
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Latest Research on the Swine Pseudorabies

A special issue of Veterinary Sciences (ISSN 2306-7381).

Deadline for manuscript submissions: closed (15 March 2024) | Viewed by 8266

Special Issue Editors

Dr. Yongbo Yang

E-Mail Website
Guest Editor
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
Interests: pseudorabies; infection; virus-host interactions; single virus tracking; vaccine; delivery system
Dr. Chao Ye

E-Mail Website
Guest Editor
College of Veterinary Medicine, Southwest University, Chongqing 400715, China
Interests: pseudorabies virus; infection and immunity; virus–host interactions; genetic variation

Special Issue Information

Dear Colleagues,

Pseudorabies (Aujeszky's disease), caused by pseudorabies virus (PRV), is one of the most important swine infectious diseases, and is responsible for considerable economic losses in the global swine industry. Although swine is the natural host of PRV, increasing evidence has suggested that PRV can spread across the species barrier to infect humans. Therefore, the prevalence of pseudorabies in swine also poses a potential threat to public health. Hence, studies on the epidemiology, pathogenesis, diagnosis and treatment of pseudorabies virus are urgently needed, and are also critical for the development of efficient prevention strategies against PRV infections.

This Special Issue, "Latest Research on Swine Pseudorabies", aims to gather the recent advances from studies focused on different aspects of pseudorabies, including epidemiology, pathogenesis, infection and immunity, structural biology, diagnosis, vaccination and antiviral therapy. All researchers working in these fields are encouraged to contribute original research articles or reviews to this Special Issue of Veterinary Sciences.

Dr. Yongbo Yang
Dr. Chao Ye
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Veterinary Sciences is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • pseudorabies
  • virus–host interactions
  • vaccine
  • genetic variation
  • infection and immunity
  • epidemiology
  • pathogenesis
  • diagnosis
  • antiviral

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Published Papers (3 papers)

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Research

11 pages, 6517 KiB  
Article
Immunogenicity Characterization of the Recombinant gI Protein Fragment from Pseudorabies Virus and an Evaluation of Its Diagnostic Use in Pigs
by Haijuan He, Baojie Qi, Yongbo Yang, Xiaowen Cui, Tianfeng Chen, Xuehui Cai, Tongqing An and Shujie Wang
Vet. Sci. 2023, 10(8), 506; https://doi.org/10.3390/vetsci10080506 - 5 Aug 2023
Cited by 1 | Viewed by 1694
Abstract
Serological testing is an important method for the diagnosis of pseudorabies virus (PRV) infection. We aimed to investigate the envelope glycoprotein I (gI) of PRV, a strong immunogen, and its potential as an efficient and low-cost diagnostic reagent. In this study, the DNA [...] Read more.
Serological testing is an important method for the diagnosis of pseudorabies virus (PRV) infection. We aimed to investigate the envelope glycoprotein I (gI) of PRV, a strong immunogen, and its potential as an efficient and low-cost diagnostic reagent. In this study, the DNA of the PRV SC strain was used as the template, and the recombinant fragment of gI (633 bp) was amplified via PCR using synthetic primers, and was then ligated into the pET-30a expression vector. The constructs were transferred into Escherichia coli (E. coli) for prokaryotic expression, and the antigenicity of the expression products was identified by Western blot analysis with pig positive serum against PRV. The recombinant protein was purified by a Ni column, and BALB/c mice were immunized with purified gI protein to obtain anti-gI-positive serum. After PK-15 cells had been infected by PRV for 48 h, the immunogenicity of purified gI protein was identified with a fluorescence immunoassay using anti-gI mouse serum. The recombinant plasmid (pET-30a-gI) was expressed, and the native gI protein was obtained after denaturation by urea and renaturation by dialysis. A small-scale ELISA test containing 1.0 µg/mL of purified gI protein was designed to evaluate pig serum (80 samples), and the results of the ELISA test were compared to those of competitive ELISA (cELISA) tests using IDEXX Kits, which resulted in 97.5% consistency. The results suggested that the truncated gI protein may be a potential diagnostic reagent. Full article
(This article belongs to the Special Issue Latest Research on the Swine Pseudorabies)
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12 pages, 2643 KiB  
Article
Preparation and Identification of a Monoclonal Antibody against the Pseudorabies Virus gE Glycoprotein through a Novel Strategy
by Zhenyang Guo, Siyu Zhang, Hu Xu, Wansheng Li, Chao Li, Jing Zhao, Bangjun Gong, Qi Sun, Lirun Xiang, Hongyuan Zhao, Qian Wang, Guohui Zhou, Yandong Tang, Tongqing An, Xuehui Cai, Zhijun Tian, Hongliang Zhang and Jinmei Peng
Vet. Sci. 2023, 10(2), 133; https://doi.org/10.3390/vetsci10020133 - 9 Feb 2023
Cited by 3 | Viewed by 2316
Abstract
Since 2011, pseudorabies virus (PRV) has recurred in several vaccinated pig farms in China. PRV variants with high virulence were found to be the main cause of the outbreaks. In the face of the PRV epidemic, detection of the wild strain is as [...] Read more.
Since 2011, pseudorabies virus (PRV) has recurred in several vaccinated pig farms in China. PRV variants with high virulence were found to be the main cause of the outbreaks. In the face of the PRV epidemic, detection of the wild strain is as important as vaccine immunization, so we hoped to achieve differential diagnosis of PRV by obtaining a monoclonal antibody (mAB) that could be used to identify the wild strain. In this study, we used a novel immunization and screening strategy to prepare an mAB and obtained mAB 1H5 against the gE glycoprotein. An immunofluorescence assay (IFA) revealed that this mAB was specific to both classic and variant strains of PRV. Subsequently, we further identified the linear epitopes of B cells recognized using the mAB. The mAB 1H5 bound at 67RRAG70, which is a novel epitope and is conserved in almost all PRV strains. These findings provide novel insight into the structure and function of PRV proteins, the analysis of antigenic epitope characteristics, and the establishment of antigen or antibody detection methods. Full article
(This article belongs to the Special Issue Latest Research on the Swine Pseudorabies)
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14 pages, 3370 KiB  
Article
Dihydromyricetin Inhibits Pseudorabies Virus Multiplication In Vitro by Regulating NF-κB Signaling Pathway and Apoptosis
by Xufan Zhao, Yaqin Chen, Wenrui Zhang, Hui Zhang, Yilong Hu, Fengyu Yang, Yingying Zhang and Xu Song
Vet. Sci. 2023, 10(2), 111; https://doi.org/10.3390/vetsci10020111 - 2 Feb 2023
Cited by 2 | Viewed by 2658
Abstract
Pseudorabies virus (PRV) infections have caused huge economic losses to the breeding industry worldwide, especially pig husbandry. PRV could threaten human health as an easily ignored zoonotic pathogen. The emergence of new mutants significantly reduced the protective effect of vaccination, indicating an urgent [...] Read more.
Pseudorabies virus (PRV) infections have caused huge economic losses to the breeding industry worldwide, especially pig husbandry. PRV could threaten human health as an easily ignored zoonotic pathogen. The emergence of new mutants significantly reduced the protective effect of vaccination, indicating an urgent need to develop specific therapeutic drugs for PRV infection. In this study, we found that dihydromyricetin (DMY) could dose-dependently restrain PRV infection in vitro with an IC50 of 161.34 μM; the inhibition rate of DMY at a concentration of 500 μM was 92.16 %. Moreover, the mode of action showed that DMY directly inactivated PRV virion and inhibited viral adsorption and cellular replication. DMY treatment could improve PRV-induced abnormal changes of the NF-κB signaling pathway and excessive inflammatory response through regulation of the contents of IκBα and p-P65/P65 and the transcriptional levels of cytokines (TNF-α, IL-1β and IL-6). Furthermore, DMY promoted the apoptosis of PRV-infected cells through the regulation of the expressions of Bax and Bcl-xl and the transcriptional levels of Caspase-3, Bax, Bcl-2 and Bcl-xl, thereby limiting the production of progeny virus. These findings indicated that DMY could be a candidate drug for the treatment of PRV infection. Full article
(This article belongs to the Special Issue Latest Research on the Swine Pseudorabies)
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