Current Method and Perspective in Animal Reproduction

A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Reproduction and Obstetrics".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1647

Special Issue Editors


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Guest Editor
College of Animal Sciences, Jilin University, Changchun 130062, China
Interests: embryo engineering; developmental biology; stem cell biology

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Guest Editor
College of Animal Sciences, Jilin University, Changchun 130062, China
Interests: embryo engineering; developmental biology; gene editing

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Guest Editor
College of Animal Sciences, Jilin University, Changchun 130062, China
Interests: animal reproductive regulation; development and application of new technologies for animal reproduction
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Guest Editor
Department of Companion Animal & Animal Resources Science, Joongbu University, Geumsan 32713, Republic of Korea
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Special Issue Information

Dear Colleagues,

This Special Issue, titled “Current Method and Perspective in Animal Reproduction”, highlights recent advances and emerging trends in the field of animal reproductive science. As reproductive efficiency remains a cornerstone of livestock production, conservation biology, and veterinary medicine, innovative technologies and updated strategies are crucial for improving fertility, understanding reproductive physiology, and addressing reproductive disorders across species. This Special Issue features original research and review articles covering diverse topics, including assisted reproductive technologies (ART), gamete biology, cryopreservation, hormonal regulation, reproductive genetics, and fertility biomarkers. Contributions also explore novel insights into reproductive biotechnology, artificial insemination, embryo transfer, and species-specific reproductive challenges. By presenting a comprehensive overview of current methodologies and forward-looking perspectives, this Special Issue aims to support researchers, veterinarians, and breeders in optimizing reproductive outcomes and promoting sustainable animal production and conservation.

Dr. Yongxun Jin
Prof. Dr. Shuang Liang
Prof. Dr. Chunjin Li
Dr. Yeon Woo Jeong
Guest Editors

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Keywords

  • animal reproduction
  • assisted reproductive technologies
  • fertility
  • gamete biology
  • embryo transfer
  • artificial insemination
  • reproductive physiology
  • reproductive biotechnology
  • cryopreservation
  • hormonal regulation

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Published Papers (2 papers)

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Research

16 pages, 2432 KB  
Article
Effects of Supplementation with Chlorogenic Acid-Rich Extract from Eucommia ulmoides Oliver During Peri-Implantation on the Reproductive Performance and Gut Microbiota of Sows
by Yan Zhang, Hexuan Qu, Hongda Pan, Dao Xiang, Seongho Choi and Shuang Liang
Vet. Sci. 2025, 12(9), 857; https://doi.org/10.3390/vetsci12090857 - 4 Sep 2025
Viewed by 575
Abstract
Chlorogenic acid (CGA)-rich extracts from Eucommia ulmoides Oliver (CAE) are known for their gut health and antioxidant benefits in livestock. This study examines the effects of CAE supplementation during the peri-implantation period on sow reproductive performance and the gut microbiota. Sixty Dongliao black [...] Read more.
Chlorogenic acid (CGA)-rich extracts from Eucommia ulmoides Oliver (CAE) are known for their gut health and antioxidant benefits in livestock. This study examines the effects of CAE supplementation during the peri-implantation period on sow reproductive performance and the gut microbiota. Sixty Dongliao black sows were randomized to receive either no supplementation (control) or CAE at 600 or 2000 mg/kg daily from gestation day −5 through day 15. High-dose CAE intake significantly increased total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), immunoglobulin A (IgA), and immunoglobulin M (IgM) levels in sow serum but decreased malondialdehyde (MDA) levels. Fecal short-chain fatty acids (SCFAs) also increase significantly. These changes correlate with improved reproductive performance, including a larger litter size, higher numbers of live-born piglets, a greater individual birth weight of live-born piglets, a higher total litter birth weight of live-born piglets, and a lower mortality rate. 16S rRNA sequencing of the fecal microbiota revealed that CAE markedly altered microbial diversity and composition, reducing the abundance of potentially harmful bacteria but increasing the abundance of beneficial bacteria. In conclusion, supplementation with CAE during the peri-implantation phase can reduce oxidative stress, alter the gut microbiota composition, and improve sow reproductive performance, thus potentially increasing breeding farm profitability. Full article
(This article belongs to the Special Issue Current Method and Perspective in Animal Reproduction)
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13 pages, 3720 KB  
Article
Efficacy of Two Estrus Synchronization Protocols in Crossbred Gyr Dairy Cows and Their Relationship with Heat Stress in the Peruvian Tropics
by Ronald W. Vásquez-Tarrillo, José E. Hernández-Guevara, Benjamín A. Depaz-Hizo, Gustavo Ampuero-Trigoso, Annie Y. Poclín-Rojas, Marisol Rojas-Reategui, Gleni T. Segura Portocarrero, Jorge D. Juarez-Moreno and Hurley A. Quispe-Ccasa
Vet. Sci. 2025, 12(9), 804; https://doi.org/10.3390/vetsci12090804 - 25 Aug 2025
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Abstract
The performance of estrus synchronization protocols may be influenced by stressful environmental conditions, even in tropically adapted cattle. This study evaluated the efficacy of two synchronization protocols for fixed-time artificial insemination (FTAI) in crossbred Gyr dairy cows and their relationship with heat stress [...] Read more.
The performance of estrus synchronization protocols may be influenced by stressful environmental conditions, even in tropically adapted cattle. This study evaluated the efficacy of two synchronization protocols for fixed-time artificial insemination (FTAI) in crossbred Gyr dairy cows and their relationship with heat stress under Peruvian tropical conditions. Multiparous lactating cows under semi-intensive systems were synchronized and divided into two groups: the conventional protocol (PC9) and the prolonged proestrus protocol (J-Synch6). Estrus manifestation, pregnancy rate, and their association with the temperature–humidity index (THI) were analyzed across seven phases of the FTAI program (73 days). In PC9 cows, no significant effect of the number of hours in each heat stress category according to THI was found. Conversely, in J-Synch6 cows, more hours in Category 0 (THI < 72) during Phases 1 to 4 (follicular emergence to preovulatory follicle) had a positive effect on the estrus manifestation, whereas in Phase 3 (follicular growth), higher exposure to Category 3 (THI ≥ 83) had a negative effect on estrus manifestation. Both protocols demonstrated similar estrus and pregnancy rates, although heat stress affected estrus rates in J-Synch6, but not pregnancy. The choice of protocol should account for heat stress risks during specific periods of the year to maximize success. Full article
(This article belongs to the Special Issue Current Method and Perspective in Animal Reproduction)
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