Special Issue "Extraction and Isolation of Compounds from Food or Natural Products"

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Food and Beverages".

Deadline for manuscript submissions: closed (30 April 2022) | Viewed by 660

Special Issue Editor

Dr. Jun Dang
E-Mail Website
Guest Editor
Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
Interests: food or natural products; chromatographic recognition of active compounds; purification and preparative isolation; new natural products and bioactivity

Special Issue Information

Dear Colleagues,

Compounds from natural products possess unique chemical structures and pharmacological activities, which help in the discovery of novel chemical structures as well as drug precursors. Traditionally, compounds were purified from natural products using open column chromatography-based separation techniques, followed by testing of their activity. However, such methods were time-consuming, strenuous, and labor-intensive and required special skills. Thus, there was an urgent need to develop an efficient, convenient, and rapid method to recognize and isolate compounds from complex natural products.

As for purification compounds, preparative high-performance liquid chromatography (prep-HPLC) is widely used due to its online detection, high selectivity, excellent reproducibility, as well as wide application range. This technique has been widely used for the purification of phenolics, flavonoids, alkaloids, phenylpropanoids, and iridoid glucosides from food or plant extracts.

The main goals of the Special Issue are to publish the development of new technologies and methods in preparative isolation of bioactive compounds from food or natural products. Compounds with new structures from food or natural products are also welcomed.

I hope this Special Issue will provide readers with a selection of papers that represent the current state of knowledge on new technologies and methods in preparative isolation of bioactive compounds from food or natural products.

Dr. Jun Dang
Guest Editor

Manuscript Submission Information

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Keywords

  • Chromatographic recognition of active compounds
  • New technologies and methods
  • Preparative isolation of compounds
  • Food or natural products
  • Purification and preparative isolation
  • New natural products and bioactivity
  • LC-MS characterization of active compounds
  • Two-dimensional or multidimensional chromatography separation technology

Published Papers (1 paper)

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Research

Article
Preparation and Antioxidant Activities of Phenylethanoids from Dracocephalum heterophyllum
Separations 2022, 9(5), 111; https://doi.org/10.3390/separations9050111 - 28 Apr 2022
Viewed by 321
Abstract
The health benefits of Dracocephalum heterophyllum are widely reported in traditional Tibetan medicines, but the reported chemical composition is limited, probably due to difficulties in separating and purifying compounds. In this study, antioxidative phenylethanoids were isolated from an extract of Dracocephalum heterophyllum using [...] Read more.
The health benefits of Dracocephalum heterophyllum are widely reported in traditional Tibetan medicines, but the reported chemical composition is limited, probably due to difficulties in separating and purifying compounds. In this study, antioxidative phenylethanoids were isolated from an extract of Dracocephalum heterophyllum using medium- and high-pressure liquid chromatography, coupled with on-line HPLC–1,1-diphenyl-2-picrylhydrazyl recognition. Firstly, crude samples (1.3 kg) of Dracocephalum heterophyllum were pretreated via silica gel medium-pressure liquid chromatography to yield 994.0 g of Fr2, of which 10.8 g was then pretreated via MCI GEL®CHP20P medium-pressure liquid chromatography. The resulting Fr23 and Fr25 were further separated and purified using high-pressure liquid chromatography, and yielded 8.08 mg of Fr2391, 9.76 mg of Fr2551, 16.09 mg of Fr2581, and 8.75 mg of Fr2582. Furthermore, analysis of the purity and structures of the phenylethanoids suggested that Fr2391, Fr2551, Fr2581, and Fr2582 corresponded to decaffeoylverbascoside, rosmarinic acid, acteoside, and 2′-O-acetylplantamajoside, respectively, with all being over 95% pure. Finally, the antioxidant potential of the compounds was explored based on their ability to scavenge 1,1-diphenyl-2-picrylhydrazine, as well as through molecular docking of proteins related to antioxidant pathways. Altogether, our findings revealed that the proposed method is promising for separating pure antioxidative phenylethanoids from other natural compounds. Full article
(This article belongs to the Special Issue Extraction and Isolation of Compounds from Food or Natural Products)
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