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443 KiB

Open AccessArticle

A Simple Small Size and Low Cost Sensor Based on Surface Plasmon Resonance for Selective Detection of Fe(III)

by Nunzio Cennamo, Giancarla Alberti, Maria Pesavento, Girolamo D'Agostino, Federico Quattrini, Raffaela Biesuz and Luigi Zeni

Sensors 2014, 14(3), 4657-4671; https://doi.org/10.3390/s140304657 - 07 Mar 2014

Cited by 50 | Viewed by 6656

Abstract

A simple, small size, and low cost sensor based on a Deferoxamine Self Assembled Monolayer (DFO-SAM) and Surface Plasmon Resonance (SPR) transduction, in connection with a Plastic Optical Fiber (POF), has been developed for the selective detection of Fe(III). DFO-SAM sensors based on [...] Read more.

A simple, small size, and low cost sensor based on a Deferoxamine Self Assembled Monolayer (DFO-SAM) and Surface Plasmon Resonance (SPR) transduction, in connection with a Plastic Optical Fiber (POF), has been developed for the selective detection of Fe(III). DFO-SAM sensors based on appropriate electrochemical techniques can be frequently found in the scientific literature. In this work, we present the first example of a DFO-SAM sensor based on SPR in an optical fiber. The SPR sensing platform was realized by removing the cladding of a plastic optical fiber along half the circumference, spin coating a buffer of Microposit S1813 photoresist on the exposed core, and finally sputtering a thin gold film. The hydroxamate siderophore deferoxamine (DFO), having high binding affinity for Fe(III), is then used in its immobilized form, as self-assembled monolayer on the gold layer surface of the POF sensor. The results showed that the DFO-SAM-POF-sensor was able to sense the formation of the Fe(III)/DFO complex in the range of concentrations between 1 μm and 50 μm with a linearity range from 0 to 30 μm of Fe(III). The selectivity of the sensor was also proved by interference tests. Full article

(This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors)

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865 KiB

Open AccessArticle

Direct Detection of Protein Biomarkers in Human Fluids Using Site-Specific Antibody Immobilization Strategies

by Maria Soler, M.-Carmen Estevez, Mar Alvarez, Marinus A. Otte, Borja Sepulveda and Laura M. Lechuga

Sensors 2014, 14(2), 2239-2258; https://doi.org/10.3390/s140202239 - 29 Jan 2014

Cited by 66 | Viewed by 12527

Abstract

Design of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When [...] Read more.

Design of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When applied in direct immunoassays, their analytical features depend strongly on the antibody immobilization strategy. A strategy for correct immobilization of antibodies based on the use of ProLinker™ has been evaluated and optimized in terms of sensitivity, selectivity, stability and reproducibility. Special effort has been focused on avoiding antibody manipulation, preventing nonspecific adsorption and obtaining a robust biosurface with regeneration capabilities. ProLinker™-based approach has demonstrated to fulfill those crucial requirements and, in combination with PEG-derivative compounds, has shown encouraging results for direct detection in biological fluids, such as pure urine or diluted serum. Furthermore, we have implemented the ProLinker™ strategy to a novel nanoplasmonic-based biosensor resulting in promising advantages for its application in clinical and biomedical diagnosis. Full article

(This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors)

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461 KiB

Open AccessArticle

Label-Enhanced Surface Plasmon Resonance: A New Concept for Improved Performance in Optical Biosensor Analysis

by Niko Granqvist, Anders Hanning, Lars Eng, Jussi Tuppurainen and Tapani Viitala

Sensors 2013, 13(11), 15348-15363; https://doi.org/10.3390/s131115348 - 08 Nov 2013

Cited by 30 | Viewed by 8492

Abstract

Surface plasmon resonance (SPR) is a well-established optical biosensor technology with many proven applications in the study of molecular interactions as well as in surface and material science. SPR is usually applied in the label-free mode which may be advantageous in cases where [...] Read more.

Surface plasmon resonance (SPR) is a well-established optical biosensor technology with many proven applications in the study of molecular interactions as well as in surface and material science. SPR is usually applied in the label-free mode which may be advantageous in cases where the presence of a label may potentially interfere with the studied interactions per se. However, the fundamental challenges of label-free SPR in terms of limited sensitivity and specificity are well known. Here we present a new concept called label-enhanced SPR, which is based on utilizing strongly absorbing dye molecules in combination with the evaluation of the full shape of the SPR curve, whereby the sensitivity as well as the specificity of SPR is significantly improved. The performance of the new label-enhanced SPR method was demonstrated by two simple model assays: a small molecule assay and a DNA hybridization assay. The small molecule assay was used to demonstrate the sensitivity enhancement of the method, and how competitive assays can be used for relative affinity determination. The DNA assay was used to demonstrate the selectivity of the assay, and the capabilities in eliminating noise from bulk liquid composition variations. Full article

(This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors)

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614 KiB

Open AccessArticle

ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging

by Nongluck Houngkamhang, Apirom Vongsakulyanon, Patjaree Peungthum, Krisda Sudprasert, Pimpun Kitpoka, Mongkol Kunakorn, Boonsong Sutapun, Ratthasart Amarit, Armote Somboonkaew and Toemsak Srikhirin

Sensors 2013, 13(9), 11913-11922; https://doi.org/10.3390/s130911913 - 09 Sep 2013

Cited by 39 | Viewed by 8973

Abstract

In this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging) technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, [...] Read more.

In this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging) technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, and single clones of anti-A and anti-B, were used to construct the five-line detection arrays using a multichannel flow cell in the SPR imager. The red blood cell (RBC) samples were applied to a multichannel flow cell that was orthogonal to the detection line arrays for blood group typing. We found that the blood samples were correctly grouped in less than 12 min by the SPR imaging technique, and the results were consistent with those of the standard agglutination technique for all 60 samples. We found that mixed clones of antibodies provided 33%–68% greater change in the SPR signal than the single-clone antibodies. Applying the SPR imaging technique using readily available antibodies may reduce the costs of the antibodies, shorten the measurement time, and increase the throughput. Full article

(This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors)

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Review

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855 KiB

Open AccessReview

Recent Advances in Plasmonic Sensors

by Lianming Tong, Hong Wei, Shunping Zhang and Hongxing Xu

Sensors 2014, 14(5), 7959-7973; https://doi.org/10.3390/s140507959 - 05 May 2014

Cited by 182 | Viewed by 11295

Abstract

Plasmonic sensing has been an important multidisciplinary research field and has been extensively used in detection of trace molecules in chemistry and biology. The sensing techniques are typically based on surface-enhanced spectroscopies and surface plasmon resonances (SPRs). This review article deals with some [...] Read more.

Plasmonic sensing has been an important multidisciplinary research field and has been extensively used in detection of trace molecules in chemistry and biology. The sensing techniques are typically based on surface-enhanced spectroscopies and surface plasmon resonances (SPRs). This review article deals with some recent advances in surface-enhanced Raman scattering (SERS) sensors and SPR sensors using either localized surface plasmon resonances (LSPRs) or propagating surface plasmon polaritons (SPPs). The advances discussed herein present some improvements in SERS and SPR sensing, as well as a new type of nanowire-based SPP sensor. Full article

(This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors)

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1252 KiB

Open AccessReview

Synthesis of Silver Nanostructures by Multistep Methods

by Tong Zhang, Yuan-Jun Song, Xiao-Yang Zhang and Jing-Yuan Wu

Sensors 2014, 14(4), 5860-5889; https://doi.org/10.3390/s140405860 - 25 Mar 2014

Cited by 67 | Viewed by 13597

Abstract

The shape of plasmonic nanostructures such as silver and gold is vital to their physical and chemical properties and potential applications. Recently, preparation of complex nanostructures with rich function by chemical multistep methods is the hotspot of research. In this review we introduce [...] Read more.

The shape of plasmonic nanostructures such as silver and gold is vital to their physical and chemical properties and potential applications. Recently, preparation of complex nanostructures with rich function by chemical multistep methods is the hotspot of research. In this review we introduce three typical multistep methods to prepare silver nanostructures with well-controlled shapes, including the double reductant method, etching technique and construction of core-shell nanostructures. The growth mechanism of double the reductant method is that different favorable facets of silver nanocrystals are produced in different reductants, which can be used to prepare complex nanostructures such as nanoflags with ultranarrow resonant band bandwidth or some silver nanostructures which are difficult to prepare using other methods. The etching technique can selectively remove nanoparticles to achieve the aim of shape control and is widely used for the synthesis of nanoflowers and hollow nanostructures. Construction of core-shell nanostructures is another tool to control shape and size. The three methods can not only prepare various silver nanostructures with well-controlled shapes, which exhibit unique optical properties, such as strong surface-enhanced Raman scattering (SERS) signal and localized surface plasmon resonance (LSPR) effect, but also have potential application in many areas. Full article

(This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors)

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589 KiB

Open AccessReview

Surface Plasmon Resonance for Cell-Based Clinical Diagnosis

by Yuhki Yanase, Takaaki Hiragun, Kaori Ishii, Tomoko Kawaguchi, Tetsuji Yanase, Mikio Kawai, Kenji Sakamoto and Michihiro Hide

Sensors 2014, 14(3), 4948-4959; https://doi.org/10.3390/s140304948 - 11 Mar 2014

Cited by 110 | Viewed by 14905

Abstract

Non-invasive real-time observations and the evaluation of living cell conditions and functions are increasingly demanded in life sciences. Surface plasmon resonance (SPR) sensors detect the refractive index (RI) changes on the surface of sensor chips in label-free and on a real-time basis. Using [...] Read more.

Non-invasive real-time observations and the evaluation of living cell conditions and functions are increasingly demanded in life sciences. Surface plasmon resonance (SPR) sensors detect the refractive index (RI) changes on the surface of sensor chips in label-free and on a real-time basis. Using SPR sensors, we and other groups have developed techniques to evaluate living cells’ reactions in response to stimuli without any labeling in a real-time manner. The SPR imaging (SPRI) system for living cells may visualize single cell reactions and has the potential to expand application of SPR cell sensing for clinical diagnosis, such as multi-array cell diagnostic systems and detection of malignant cells among normal cells in combination with rapid cell isolation techniques. Full article

(This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors)

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369 KiB

Open AccessReview

Plasmonic Sensors Based on Doubly-Deposited Tapered Optical Fibers

by Agustín González-Cano, María-Cruz Navarrete, Óscar Esteban and Natalia Díaz-Herrera

Sensors 2014, 14(3), 4791-4805; https://doi.org/10.3390/s140304791 - 10 Mar 2014

Cited by 28 | Viewed by 5848

Abstract

A review of the surface plasmon resonance (SPR) transducers based on tapered fibers that have been developed in the last years is presented. The devices have proved their good performance (specifically, in terms of sensitivity) and their versatility and they are a very [...] Read more.

A review of the surface plasmon resonance (SPR) transducers based on tapered fibers that have been developed in the last years is presented. The devices have proved their good performance (specifically, in terms of sensitivity) and their versatility and they are a very good option to be considered as basis for any kind of chemical and biological sensor. The technology has now reached its maturity and here we summarize some of the characteristics of the devices produced. Full article

(This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors)

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