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Biomolecular Engineering for Diagnostic Applications II
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Biomolecular Engineering for Diagnostic Applications II

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biomedical Sensors".

Deadline for manuscript submissions: closed (20 November 2022) | Viewed by 10134

Special Issue Editor

Dr. Ki Soo Park

E-Mail Website
Guest Editor
Department of Biological Engineering, Konkuk University, Seoul 05029, Korea
Interests: nucleic acid engineering; exosome analysis; molecular diagnostics; biosensor; nanobiotechnology; biomarker discovery
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

To quote Alain Mérieux, chairman of Institut Mérieux, “Without diagnostics, medicine is blind”. We cannot obtain the appropriate treatment for a disease without knowing what its cause is. As this phrase implies, then, diagnostics are an essential part of healthcare at both individual and community levels. Highly sensitive, fast, and cost-effective diagnostic systems can have far-reaching applications in medicine as well as environmental and food analysis. Many research efforts have been made to develop such platforms by bringing together diverse fields, including chemistry, biotechnology, and nanotechnology.

In this Special Issue, we aim to explore recent advances in molecular diagnostic technologies that can revolutionize human wellbeing. We encourage the submission of papers in the following topics described by the keywords below but also welcome works on related topics.

Dr. Ki Soo Park
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nucleic acids
  • isothermal amplification
  • molecular diagnostics
  • biosensors
  • biomarkers
  • cancer
  • infectious disease
  • exosome
  • antimicrobial resistance
  • clinical applications

Published Papers (4 papers)

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Research

9 pages, 2940 KiB  
Article
Pyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids
by Seokhwan Kim, Jinjoo Han, Heeseok Chung, Yong-Keun Choi, Ayemeh Bagheri Hashkavayi, Yu Zhou and Ki Soo Park
Sensors 2021, 21(22), 7567; https://doi.org/10.3390/s21227567 - 14 Nov 2021
Cited by 7 | Viewed by 2199
Abstract
In recent years, cerium oxide (CeO2) nanoparticles (NPs) have drawn significant attention owing to their intrinsic enzyme mimetic properties, which make them powerful tools for biomolecular detection. In this work, we evaluated the effect of pyrophosphate (PPi) on the oxidase activity [...] Read more.
In recent years, cerium oxide (CeO2) nanoparticles (NPs) have drawn significant attention owing to their intrinsic enzyme mimetic properties, which make them powerful tools for biomolecular detection. In this work, we evaluated the effect of pyrophosphate (PPi) on the oxidase activity of CeO2 NPs. The presence of PPi was found to enhance the oxidase activity of CeO2 NPs, with enhanced colorimetric signals. This particular effect was then used for the colorimetric detection of target nucleic acids. Overall, the PPi-enhanced colorimetric signals of CeO2 NPs oxidase activity were suppressed by the presence of the target nucleic acids. Compared with previous studies using CeO2 NPs only, our proposed system significantly improved the signal change (ca. 200%), leading to more sensitive and reproducible colorimetric analysis of target nucleic acids. As a proof-of-concept study, the proposed system was successfully applied to the highly selective and sensitive detection of polymerase chain reaction products derived from Klebsiella pneumoniae. Our findings will benefit the rapid detection of nucleic acid biomarkers (e.g., pathogenic bacterial DNA or RNA) in point-of-care settings. Full article
(This article belongs to the Special Issue Biomolecular Engineering for Diagnostic Applications II)
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10 pages, 1344 KiB  
Article
Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose Meter
by Hyogu Han, Junhyun Park and Jun Ki Ahn
Sensors 2021, 21(19), 6396; https://doi.org/10.3390/s21196396 - 24 Sep 2021
Cited by 5 | Viewed by 2440
Abstract
We herein describe a cascade enzymatic reaction (CER)-based IgE detection method utilizing a personal glucose meter (PGM), which relies on alkaline phosphatase (ALP) activity that regulates the amount of adenosine triphosphate (ATP). The amount of sandwich assay complex is determined according to the [...] Read more.
We herein describe a cascade enzymatic reaction (CER)-based IgE detection method utilizing a personal glucose meter (PGM), which relies on alkaline phosphatase (ALP) activity that regulates the amount of adenosine triphosphate (ATP). The amount of sandwich assay complex is determined according to the presence or absence of the target IgE. Additionally, the ALP in the sandwich assay catalyzes the dephosphorylation of ATP, a substrate of CER, which results in the changes in glucose level. By employing this principle, IgE was reliably detected at a concentration as low as ca. 29.6 ng/mL with high specificity toward various proteins. Importantly, the limit of detection (LOD) of this portable PGM-based approach was comparable to currently commercialized ELISA kit without expensive and bulky analysis equipment as well as complexed washing step. Finally, the diagnostic capability of this method was also successfully verified by reliably detecting IgE present in a real human serum sample with an excellent recovery ratio within 100 ± 6%. Full article
(This article belongs to the Special Issue Biomolecular Engineering for Diagnostic Applications II)
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11 pages, 7849 KiB  
Article
Three-Way Junction-Induced Isothermal Amplification with High Signal-to-Background Ratio for Detection of Pathogenic Bacteria
by Jung Ho Kim, Seokjoon Kim, Sung Hyun Hwang, Tae Hwi Yoon, Jung Soo Park, Eun Sung Lee, Jisu Woo and Ki Soo Park
Sensors 2021, 21(12), 4132; https://doi.org/10.3390/s21124132 - 16 Jun 2021
Cited by 5 | Viewed by 2532
Abstract
The consumption of water and food contaminated by pathogens is a major cause of numerous diseases and deaths globally. To control pathogen contamination and reduce the risk of illness, a system is required that can quickly detect and monitor target pathogens. We developed [...] Read more.
The consumption of water and food contaminated by pathogens is a major cause of numerous diseases and deaths globally. To control pathogen contamination and reduce the risk of illness, a system is required that can quickly detect and monitor target pathogens. We developed a simple and reproducible strategy, termed three-way junction (3WJ)-induced transcription amplification, to detect target nucleic acids by rationally combining 3WJ-induced isothermal amplification with a light-up RNA aptamer. In principle, the presence of the target nucleic acid generates a large number of light-up RNA aptamers (Spinach aptamers) through strand displacement and transcription amplification for 2 h at 37 °C. The resulting Spinach RNA aptamers specifically bind to fluorogens such as 3,5-difluoro-4-hydroxybenzylidene imidazolinone and emit a highly enhanced fluorescence signal, which is clearly distinguished from the signal emitted in the absence of the target nucleic acid. With the proposed strategy, concentrations of target nucleic acids selected from the genome of Salmonellaenterica serovar Typhi (S. Typhi) were quantitatively determined with high selectivity. In addition, the practical applicability of the method was demonstrated by performing spike-and-recovery experiments with S. Typhi in human serum. Full article
(This article belongs to the Special Issue Biomolecular Engineering for Diagnostic Applications II)
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12 pages, 2025 KiB  
Article
Multiplexed Affinity Measurements of Extracellular Vesicles Binding Kinetics
by Elisa Chiodi, George G. Daaboul, Allison M. Marn and M. Selim Ünlü
Sensors 2021, 21(8), 2634; https://doi.org/10.3390/s21082634 - 09 Apr 2021
Cited by 6 | Viewed by 2402
Abstract
Extracellular vesicles (EVs) have attracted significant attention as impactful diagnostic biomarkers, since their properties are closely related to specific clinical conditions. However, designing experiments that involve EVs phenotyping is usually highly challenging and time-consuming, due to laborious optimization steps that require very long [...] Read more.
Extracellular vesicles (EVs) have attracted significant attention as impactful diagnostic biomarkers, since their properties are closely related to specific clinical conditions. However, designing experiments that involve EVs phenotyping is usually highly challenging and time-consuming, due to laborious optimization steps that require very long or even overnight incubation durations. In this work, we demonstrate label-free, real-time detection, and phenotyping of extracellular vesicles binding to a multiplexed surface. With the ability for label-free kinetic binding measurements using the Interferometric Reflectance Imaging Sensor (IRIS) in a microfluidic chamber, we successfully optimize the capture reaction by tuning various assay conditions (incubation time, flow conditions, surface probe density, and specificity). A single (less than 1 h) experiment allows for characterization of binding affinities of the EVs to multiplexed probes. We demonstrate kinetic characterization of 18 different probe conditions, namely three different antibodies, each spotted at six different concentrations, simultaneously. The affinity characterization is then analyzed through a model that considers the complexity of multivalent binding of large structures to a carpet of probes and therefore introduces a combination of fast and slow association and dissociation parameters. Additionally, our results confirm higher affinity of EVs to aCD81 with respect to aCD9 and aCD63. Single-vesicle imaging measurements corroborate our findings, as well as confirming the EVs nature of the captured particles through fluorescence staining of the EVs membrane and cargo. Full article
(This article belongs to the Special Issue Biomolecular Engineering for Diagnostic Applications II)
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