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Electrochemical Biosensing Devices and Their Applications

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Chemical Sensors".

Deadline for manuscript submissions: 25 May 2026 | Viewed by 595

Special Issue Editors


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Guest Editor
Department of Energy Systems, School of Technology, University of Thessaly, Geopolis, Ring-Road of Larisa-Trikala, GR-41500 Larisa, Greece
Interests: electrochemistry; photoelectrochemsitry; gas sensors; glucose; nanotechnology; bio-sensors; amperometric sensors; hydrogen; fuel cells
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Guest Editor
Institute of Biological Science, Faculty of Medicine, The John Paul II Catholic University of Lublin, Lublin, Poland
Interests: voltammetry; electrochemical detection; method development and validation; bio-sensors; biological sample analysis; determination of biomolecules
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Cutting-edge electrochemical technologies utilizing accurate and rapid data are a necessity, specifically in the healthcare, food, agriculture, environmental and defense industries. The application of electrochemical biosensing devices which could provide precise information in a better, faster, and more reliable way in these sectors is still considered a challenge. Innovative platforms, smart sensing tools, and others special technologies that offer, among other benefits, flexibility, remote sensing, and reliability should be addressed. This Special Issue thus aims to bring to light the most innovative and advanced electrochemical biosensing devices, tools, platforms, and software which enable more accurate detection. Thus, we welcome novel ideas and the latest research developments, and those aiming to be major contributions to future progress across a wide range of industrial sectors. 

Dr. Angeliki Brouzgou
Dr. Ilona Sadok
Guest Editors

Manuscript Submission Information

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Keywords

  • electrochemical sensing platforms
  • electrochemical biosensing technologies
  • electrochemical diagnostics
  • industrial sensing tools
  • smart sensing devices
  • detection tools
  • methodological improvements

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Published Papers (1 paper)

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11 pages, 1631 KB  
Technical Note
Sequential Injection Analysis of Cholesterol Using an Oxidation–Reduction Electrode Detector
by Takato Imanaka and Takashi Masadome
Sensors 2025, 25(18), 5863; https://doi.org/10.3390/s25185863 - 19 Sep 2025
Viewed by 264
Abstract
A new automated method for the determination of cholesterol in serum was developed by combining sequential injection analysis (SIA) with potentiometric detection using a gold oxidation–reduction potential (ORP) electrode because serum cholesterol is an important indicator of abnormal lipid metabolism, arteriosclerosis, and hypertension [...] Read more.
A new automated method for the determination of cholesterol in serum was developed by combining sequential injection analysis (SIA) with potentiometric detection using a gold oxidation–reduction potential (ORP) electrode because serum cholesterol is an important indicator of abnormal lipid metabolism, arteriosclerosis, and hypertension in clinical diagnosis. The method is based on enzymatic hydrolysis of cholesterol esters by cholesterol esterase (CE) to yield free cholesterol, followed by oxidation with cholesterol oxidase (COD) to produce hydrogen peroxide. In the presence of horseradish peroxidase (HRP) and potassium ferrocyanide (K4[Fe(CN)6]), hydrogen peroxide oxidizes ferrocyanide to ferricyanide (K3[Fe(CN)6]), and the concentration ratio of ferri-/ferrocyanide is determined potentiometrically. Experimental conditions were optimized as follows: 5.0 mM K4[Fe(CN)6], 2 min reaction time, 0.5 units/mL HRP, 0.75 units/mL COD for free cholesterol, 1.5 units/mL COD and 10.0 units/mL CE for total cholesterol, and 5.0% (w/v) Triton X-100 with 5.0% (v/v) isopropanol as solubilizing agents. Under these conditions, the calibration curve for total cholesterol exhibited a Nernstian slope of 47.6 mV/decade over the range of 1.0 × 10−5–1.0 × 10−3 M, with no significant interference from common serum constituents. This method offers low reagent consumption, high automation, and simple operation, making it promising for clinical cholesterol analysis. Full article
(This article belongs to the Special Issue Electrochemical Biosensing Devices and Their Applications)
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