Clinical Proteomics: Fourth Edition

Dear Colleagues,

Computational and technological advances have ushered in a new era in the field of proteomics, leading to the analysis of large numbers of samples in record time. There has also been a remarkable shift in protein quantification approaches, led primarily by the development of label-free quantification methods using either DDA- or DIA-based approaches. This is further complemented by the now-popular use of chemical labeling techniques, such as TMT, and/or targeted quantification techniques, such as MRM/SRM and, most importantly, PRM. These developments offer unprecedented opportunities to discover novel biomarker signatures and to systematically study disease mechanisms. Targeted high-resolution quantification technologies such as PRM allow for biomarker validation studies from the same or independent cohort of patients, as well as the analysis of a range of samples, from body fluids to human tissue biopsies. Despite these technological advances, there are considerable challenges to be overcome, such as (1) the robust pipelines needed for the analysis of body fluids such as serum, considering the large dynamic range and need for depletion strategies, (2) the reproducibility of DIA-based approaches for the analysis of thousands of clinical samples, and new MS sample preparation and acquisition methods.

For this Special Issue, we look forward to receiving original clinical proteomics studies and review articles focused on (1) the underlying mechanisms of disease progression using human tissues or representative cell lines, (2) biomarker discovery studies using quantitative proteomics, and (3) mass spectrometry-based biomarker validation studies, as well as (4) the use of proteomics to discover modes of action of new and existing drugs.

Dr. Vikram Sharma
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Proteomes is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

• clinical proteomics
• disease mechanisms
• biomarker discovery
• biomarker validation