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Processes
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Bioprocess Design and Biomass Production Processes
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Bioprocess Design and Biomass Production Processes

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Biological Processes and Systems".

Deadline for manuscript submissions: 30 June 2025 | Viewed by 3818

Special Issue Editors

Dr. Alex Graça Contato

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Guest Editor
Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, 12612-550, Brazil
Interests: Lignocellulolytic enzymes; Bioprocess optimization; Bioreactors design; Fermentation; Biomass valorization; Biofuels; Microbial engineering
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Rosane Peralta

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Guest Editor
Department of Biochemistry, Maringá State University, Maringá, 87020-900, Brazil
Interests: Biochemistry of microorganisms; Bioremediation; Adding value to agro-industrial waste; Production of bioactive compounds.
Special Issues, Collections and Topics in MDPI journals
Dr. Cristina Giatti Marques de Souza

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Guest Editor
Department of Biochemistry, Maringá State University, Maringá, 87020-900, Brazil
Interests: Biosorption; Pharmaceuticals; White-rot fungus; Antioxidants

Special Issue Information

Dear Colleagues,

In recent years, there has been a growing emphasis on sustainable practices in biotechnology, driven by the urgent need to address environmental concerns and meet the demands of a rapidly evolving global economy. Within this context, bioprocess design and biomass production processes play a pivotal role in harnessing the potential of biological resources for various applications, ranging from renewable energy production using biomass-derived biocatalysts and enzymes to the development of biobased materials, biofuels, and pharmaceuticals. The biomass produced can come from the most varied sources, including biomass derived from microorganisms, algae, agricultural and forestry residues, organic residues, and aquatic plants. However, maximizing the efficiency and sustainability of biomass production processes requires interdisciplinary approaches, encompassing bioprocess engineering, microbiology, metabolic engineering, and environmental science.

This special issue on “Bioprocess Design and Biomass Production Processes” seeks high-quality works focusing on bioprocess optimization, biomass cultivation, and sustainable biotechnology. Topics include, but are not limited to:

  • Bioreactor design and optimization
  • Microbial engineering for enhanced biomass production
  • Metabolic engineering for biomass composition yield improvement
  • Integration of bioprocesses with downstream processing
  • Bioprocess scale-up and techno-economic analysis
  • Biocatalysts and enzymes for biomass conversion
  • Bioprocess monitoring and control strategies
  • Sustainable feedstock selection and management
  • Development of biobased materials, biofuels, and pharmaceuticals

Dr. Alex Graça Contato
Prof. Dr. Rosane Peralta
Dr. Cristina Giatti Marques de Souza
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioprocess design
  • biomass valorization
  • fermentation
  • biorefineries
  • microbial and metabolic engineering
  • enzymes
  • biofuel production
  • biopolymer synthesis
  • waste to energy
  • algal biotechnology

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

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Research

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24 pages, 2546 KiB  
Article
Optimization of Immobilization, Characterization, and Environmental Applications of Laccases from Pycnoporus sanguineus UEM-20
by Vinícius Mateus Salvatori Cheute, Emanueli Backes, Vanesa de Oliveira Pateis, Verci Alves de Oliveira Junior, Thaís Marques Uber, José Rivaldo dos Santos Filho, Luís Felipe Oliva dos Santos, Rafael Castoldi, Cristina Giatti Marques de Souza, Julio Cesar Polonio, Alex Graça Contato, Adelar Bracht and Rosane Marina Peralta
Processes 2025, 13(6), 1800; https://doi.org/10.3390/pr13061800 - 6 Jun 2025
Viewed by 232
Abstract
The immobilization of a laccase from Pycnoporus sanguineus UEM-20 via the formation of cross-linked enzyme aggregates (CLEAs) was optimized through a central composite design (CCD) of response surface methodology (RSM). Both free and immobilized enzymes were investigated for their physico-chemical characteristics, and their [...] Read more.
The immobilization of a laccase from Pycnoporus sanguineus UEM-20 via the formation of cross-linked enzyme aggregates (CLEAs) was optimized through a central composite design (CCD) of response surface methodology (RSM). Both free and immobilized enzymes were investigated for their physico-chemical characteristics, and their adequacy in removing bisphenol A (BPA) and decolorizing malachite green dye in solution was evaluated. The immobilization caused only minor differences in thermostability. Upon immobilization, the enzyme experienced some changes in its kinetic properties. The Vmax decreased by a factor of 1.1, and the KM increased by a factor of 1.89. These kinetic changes did not modify in any remarkable way the capacity of the immobilized enzyme in degrading BPA and decolorizing malachite green dye. Its sensitivity to NaCl was also minimally affected by immobilization. However, its sensitivity to sodium sulfate was substantially decreased. After 1 month’s conservation, the activity of the free form had suffered a drastic drop. The immobilized form, by contrast, remained 100% active after 6 months. All these findings predict that the immobilized laccase from P. sanguineus UEM-20 may be useful in the enzymatic bioremediation of pollutants such as endocrine disruptors and synthetic dyes. Full article
(This article belongs to the Special Issue Bioprocess Design and Biomass Production Processes)
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Review

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43 pages, 3219 KiB  
Review
Advances in Biomass and Microbial Lipids Production: Trends and Prospects
by Gabriela dos S. Costa, Walter J. Martinez-Burgos, Guilherme A. dos Reis, Yenis P. Puche, Fernando R. Vega, Cristine Rodrigues, Josilene L. Serra, Sofia de M. Campos and Carlos R. Soccol
Processes 2024, 12(12), 2903; https://doi.org/10.3390/pr12122903 - 18 Dec 2024
Cited by 2 | Viewed by 2984
Abstract
Biotechnology is increasingly being used as a tool to replace traditional production methods due to concerns about the increasing damage caused by global warming. Bacteria, yeasts, fungi, and microalgae are microorganisms able to transform residues into value-added bioproducts. They produce microbial biomass that [...] Read more.
Biotechnology is increasingly being used as a tool to replace traditional production methods due to concerns about the increasing damage caused by global warming. Bacteria, yeasts, fungi, and microalgae are microorganisms able to transform residues into value-added bioproducts. They produce microbial biomass that can result in the production of several products, such as biofuels, microbial proteins, fatty acids, bioactive polysaccharides, carotenoids, industrial enzymes, polyhydroxyalkanoates, and biofertilizers, among others. To improve microbial biomass and lipid yield, modern genetic engineering techniques can be applied as a way of optimizing processes and conditions. This review aims to explore the latest trends and technological advances in microbial biomass and lipid production, including optimization strategies for cultivation conditions and the use of genetic engineering to enhance yields and efficiency. It also discusses the challenges and future prospects for scaling up production for industrial applications. The potential of microbial lipids to contribute to a sustainable bioeconomy, as well as their applications in renewable energy and food industries, underscores their importance in a world increasingly seeking alternatives to fossil fuel dependence and unsustainable agricultural practices. Full article
(This article belongs to the Special Issue Bioprocess Design and Biomass Production Processes)
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