In Vitro Systems and Virus Elimination in Horticultural Plants and Rare Endangered Species

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: 20 September 2024 | Viewed by 756

Special Issue Editors

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Guest Editor
Plant Physiology, Immunity and Protection Department, N.V. Tsitsin Main Botanical Garden of Russian Academy of Science, 127276 Moscow, Russia
Interests: horticultural crops; rare endangered species; plant regeneration; organogenesis; somatic embryogenesis; virus diagnostics; virus-free plant obtaining; biodiversity conservation; plant genomic; abiotic and biotic stress
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Gardening and Horticulture, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Xishuangbanna 666303, China
Interests: orchid breeding and tissue culture; plant cryopreservation; plant genetic resource conservation; seed germination; horticulture

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Guest Editor
1. Department of Analytical Chemistry and Physical Chemistry, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria
2. Laboratory of Cell Biosystems, Department of Biotechnology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria
Interests: bioactive substances by plant in vitro systems; bioprocess engineering of plant in vitro systems; plant biotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In vitro systems today offer the possibility to propagate, preserve, and acclimatize cultivars and species of different horticultural plants, which are valuable as everyday food, used for cosmetics, medicine, etc. Tissue culture is a complex range of methods used in plant development for isolated plant cells, tissues, or organs cultured in different conditions. To obtain healthy plant material, different methods of virus elimination are used, such as chemotherapy, thermotherapy and cryotherapy. The methods of in vitro regeneration depend on different biotic and abiotic factors. During the study of this process, it is incredibly important to investigate the physiology, biochemistry, and anatomomorphology characteristics of cultured plants, organs, and tissues. Genomics provides us with useful information on virus diagnostics and open mechanisms of plant development at different stages of propagation and adaptation. Tissue culture has developed useful biotechnological approaches to achieve horticulture and plant biodiversity conservation.

The purpose of this Special Issue titled “In Vitro Systems and Virus Elimination in Horticultural Plants and Rare Endangered Species” is to present results in plant biotechnology, plant virology, and plant genomics using traditional and new methods, and perspectives of in vitro propagation and conservation that have been successful in several fields of tissue culture applied to horticultural and wild plants.

Prof. Dr. Irina Mitrofanova
Prof. Dr. Kanchit Thammasiri
Prof. Dr. Atanas Pavlov
Guest Editors

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • clonal micropropagation
  • organogenesis/somatic embryogenesis
  • plant cleaning up and obtain virus- and viroid-free cultivars and forms
  • genomic of virus diagnostics, plant regeneration, conservation, and acclimatization
  • anatomomorphological, physiological, and biochemical aspects of plant propagation
  • plant conservation
  • plant acclimatization ex vitro and in vivo

Published Papers (1 paper)

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18 pages, 1075 KiB  
In Vitro Collection for the Safe Storage of Grapevine Hybrids and Identification of the Presence of Plasmopara viticola Resistance Genes
by Natalya V. Romadanova, Moldir M. Aralbayeva, Alina S. Zemtsova, Alyona M. Alexandrova, Saule Zh. Kazybayeva, Natalya V. Mikhailenko, Svetlana V. Kushnarenko and Jean Carlos Bettoni
Plants 2024, 13(8), 1089; - 13 Apr 2024
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This paper focuses on the creation of an in vitro collection of grapevine hybrids from the breeding program of the Kazakh Scientific Research Institute of Fruit Growing and Viticulture and investigates the presence of Plasmopara viticola resistance mediated by Rpv3 and Rpv12 loci. [...] Read more.
This paper focuses on the creation of an in vitro collection of grapevine hybrids from the breeding program of the Kazakh Scientific Research Institute of Fruit Growing and Viticulture and investigates the presence of Plasmopara viticola resistance mediated by Rpv3 and Rpv12 loci. We looked at the optimization of in vitro establishment using either shoots taken directly from field-grown plants or from budwood cuttings forced indoors. We further screened for the presence of endophyte contamination in the initiated explants and optimized the multiplication stage. Finally, the presence of the resistance loci against P. viticola was studied. The shoots initiated from the field-sourced explants were the more effective method of providing plant sources for in vitro initiation once all plant accessions met the goal of in vitro establishment. The concentration of phytohormones and the acidity of the culture medium have a great effect on the multiplication rate and the quality of in vitro stock cultures. Out of 17 grapevine accessions, 16 showed the presence of single or combined resistance loci against P. viticola. The grapevine accessions identified as carrying Rpv3 and Rpv12 alleles represent important genetic resources for disease resistance breeding programs. These accessions may further contribute to the creation of new elite cultivars of economic interest. Full article
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: In Vitro Plant Tissue Cultivation: A Tool for Viral Clearance
Authors: Natane Amaral Miranda
Affiliation: Departamento de Silvicultura, Universidade Federal Rural Do Rio de Janeiro, Seropédica, RJ, Brazil

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