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In Vitro Systems and Virus Elimination in Horticultural Plants and...
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In Vitro Systems and Virus Elimination in Horticultural Plants and Rare Endangered Species

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: closed (20 September 2024) | Viewed by 5424

Special Issue Editors

Prof. Dr. Irina Mitrofanova

E-Mail Website
Guest Editor
Plant Physiology, Immunity and Protection Department, N.V. Tsitsin Main Botanical Garden of Russian Academy of Science, 127276 Moscow, Russia
Interests: horticultural crops; rare endangered species; plant regeneration; organogenesis; somatic embryogenesis; virus diagnostics; virus-free plant obtaining; biodiversity conservation; plant genomic; abiotic and biotic stress
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Kanchit Thammasiri

E-Mail Website
Guest Editor
Department of Gardening and Horticulture, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Xishuangbanna 666303, China
Interests: orchid breeding and tissue culture; plant cryopreservation; plant genetic resource conservation; seed germination; horticulture
Prof. Dr. Atanas Pavlov

E-Mail Website1 Website2
Guest Editor
1. Department of Analytical Chemistry and Physical Chemistry, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria
2. Laboratory of Cell Biosystems, Department of Biotechnology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria
Interests: bioactive substances by plant in vitro systems; bioprocess engineering of plant in vitro systems; plant biotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In vitro systems today offer the possibility to propagate, preserve, and acclimatize cultivars and species of different horticultural plants, which are valuable as everyday food, used for cosmetics, medicine, etc. Tissue culture is a complex range of methods used in plant development for isolated plant cells, tissues, or organs cultured in different conditions. To obtain healthy plant material, different methods of virus elimination are used, such as chemotherapy, thermotherapy and cryotherapy. The methods of in vitro regeneration depend on different biotic and abiotic factors. During the study of this process, it is incredibly important to investigate the physiology, biochemistry, and anatomomorphology characteristics of cultured plants, organs, and tissues. Genomics provides us with useful information on virus diagnostics and open mechanisms of plant development at different stages of propagation and adaptation. Tissue culture has developed useful biotechnological approaches to achieve horticulture and plant biodiversity conservation.

The purpose of this Special Issue titled “In Vitro Systems and Virus Elimination in Horticultural Plants and Rare Endangered Species” is to present results in plant biotechnology, plant virology, and plant genomics using traditional and new methods, and perspectives of in vitro propagation and conservation that have been successful in several fields of tissue culture applied to horticultural and wild plants.

Prof. Dr. Irina Mitrofanova
Prof. Dr. Kanchit Thammasiri
Prof. Dr. Atanas Pavlov
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • clonal micropropagation
  • organogenesis/somatic embryogenesis
  • plant cleaning up and obtain virus- and viroid-free cultivars and forms
  • genomic of virus diagnostics, plant regeneration, conservation, and acclimatization
  • anatomomorphological, physiological, and biochemical aspects of plant propagation
  • plant conservation
  • plant acclimatization ex vitro and in vivo

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Published Papers (3 papers)

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Research

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13 pages, 2548 KiB  
Article
Efficacy of Plant Tissue Culture Techniques for Eliminating Black Mulberry Idaeovirus (BMIV) from Infected Black Mulberry (Morus nigra)
by Doaa Waseem Abdelwahab Elansary, Kahraman Gürcan, Vahid Roumi and Özhan Şimşek
Plants 2024, 13(21), 2959; https://doi.org/10.3390/plants13212959 - 23 Oct 2024
Viewed by 1228
Abstract
Obtaining virus-free plants is a crucial step in disease management that enables reliable and profitable fruit farming. The present study applied various in vitro virus elimination protocols, including apical shoot culture, chemotherapy, thermotherapy, cryotherapy, and their combination, to eliminate black mulberry Idaeovirus (BMIV) [...] Read more.
Obtaining virus-free plants is a crucial step in disease management that enables reliable and profitable fruit farming. The present study applied various in vitro virus elimination protocols, including apical shoot culture, chemotherapy, thermotherapy, cryotherapy, and their combination, to eliminate black mulberry Idaeovirus (BMIV) from sour black mulberry. First, a shoot tip (0.5–2 mm) culture protocol was optimized, and four ribavirin concentrations (0, 10, 20, and 30 mg/L) were investigated over five weeks as a form of chemotherapy (ch). For the first thermotherapy treatment (Ch + Th1st), chemotherapy treatment was followed by a gradual increase in the temperature (24–33 °C). In another experiment (Th2nd + Ch), in vitro shoots were incubated in the dark for two weeks at two different temperatures (35 ± 1 °C and 37 ± 1 °C, for one week each). Subsequently, the shoot tips were incubated with various ribavirin doses. Finally, cryotherapy (Cr) was used with or without immersing the shoot tips in liquid nitrogen. A two-step RT-PCR was performed to assess the presence of the virus in 7–8-week-old in vitro plants. Th2nd + Ch significantly increased the shoot tip burst and plant survival/morphogenesis compared to the other treatments. Except for the application of cryotherapy, the protocols eliminated BMIV in different proportions, and the highest virus elimination rate (50%) was obtained by applying 30 mg/L ribavirin during the Ch + Th1st treatment. These findings are essential in preventing the dissemination of the virus and enabling the safe movement of germplasm around the world. Full article
(This article belongs to the Special Issue In Vitro Systems and Virus Elimination in Horticultural Plants and Rare Endangered Species)
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18 pages, 1075 KiB  
Article
In Vitro Collection for the Safe Storage of Grapevine Hybrids and Identification of the Presence of Plasmopara viticola Resistance Genes
by Natalya V. Romadanova, Moldir M. Aralbayeva, Alina S. Zemtsova, Alyona M. Alexandrova, Saule Zh. Kazybayeva, Natalya V. Mikhailenko, Svetlana V. Kushnarenko and Jean Carlos Bettoni
Plants 2024, 13(8), 1089; https://doi.org/10.3390/plants13081089 - 13 Apr 2024
Cited by 5 | Viewed by 1452
Abstract
This paper focuses on the creation of an in vitro collection of grapevine hybrids from the breeding program of the Kazakh Scientific Research Institute of Fruit Growing and Viticulture and investigates the presence of Plasmopara viticola resistance mediated by Rpv3 and Rpv12 loci. [...] Read more.
This paper focuses on the creation of an in vitro collection of grapevine hybrids from the breeding program of the Kazakh Scientific Research Institute of Fruit Growing and Viticulture and investigates the presence of Plasmopara viticola resistance mediated by Rpv3 and Rpv12 loci. We looked at the optimization of in vitro establishment using either shoots taken directly from field-grown plants or from budwood cuttings forced indoors. We further screened for the presence of endophyte contamination in the initiated explants and optimized the multiplication stage. Finally, the presence of the resistance loci against P. viticola was studied. The shoots initiated from the field-sourced explants were the more effective method of providing plant sources for in vitro initiation once all plant accessions met the goal of in vitro establishment. The concentration of phytohormones and the acidity of the culture medium have a great effect on the multiplication rate and the quality of in vitro stock cultures. Out of 17 grapevine accessions, 16 showed the presence of single or combined resistance loci against P. viticola. The grapevine accessions identified as carrying Rpv3 and Rpv12 alleles represent important genetic resources for disease resistance breeding programs. These accessions may further contribute to the creation of new elite cultivars of economic interest. Full article
(This article belongs to the Special Issue In Vitro Systems and Virus Elimination in Horticultural Plants and Rare Endangered Species)
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Review

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23 pages, 1414 KiB  
Review
Towards Pathogen-Free Coconut Germplasm Exchange
by Chongxi Yang, Van Anh Nguyen, Naga Prafulla Chandrika Nulu, Sundaravelpandian Kalaipandian, Fernanda Caro Beveridge, Julianne Biddle, Anthony Young and Steve W. Adkins
Plants 2024, 13(13), 1809; https://doi.org/10.3390/plants13131809 - 30 Jun 2024
Cited by 3 | Viewed by 1795
Abstract
Coconut (Cocos nucifera L.) is an important palm species that serves as the mainstay of several industries and contributes to the livelihoods of millions of smallholder farmers. International exchange of coconut germplasm has been undertaken for several decades to facilitate the conservation [...] Read more.
Coconut (Cocos nucifera L.) is an important palm species that serves as the mainstay of several industries and contributes to the livelihoods of millions of smallholder farmers. International exchange of coconut germplasm has been undertaken for several decades to facilitate the conservation of selected varieties within global genebanks and for the distribution to farmers and scientists. In vitro systems are a convenient and an efficient method for the exchange of coconut germplasm. However, it is possible that these tissue culture systems can transfer lethal pathogens causing a threat to the importing countries. In this review, the following topics are discussed: the major disease-causing agents of concern, the various tissues that could be used for coconut germplasm exchange, and the techniques available for the detection and elimination of disease-causing agents from various transmission systems. Additionally, the lack of clear, science-backed guidelines to facilitate the exchange of in vitro coconut materials is raised, along with recommendations for future studies to ensure the safe movement of coconut germplasm without biosecurity risks. Full article
(This article belongs to the Special Issue In Vitro Systems and Virus Elimination in Horticultural Plants and Rare Endangered Species)
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