Genome Editing Application on Plant Growth and Development Improvement

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: 30 June 2025 | Viewed by 997

Special Issue Editors


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Guest Editor
Umeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183 Umeå, Sweden
Interests: flowering-time regulation; gene editing; plant genetics and molecular biology; tree growth and phenology
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Guest Editor
Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam
Interests: plant molecular biology; environmental stress response; transcription factor; epigenetics; stress memory; vegetable genome editing

Special Issue Information

Dear Colleagues,

Genome editing is a powerful tool that allows scientists to precisely and efficiently alter an organism’s DNA. Plants growing under natural conditions often face multiple stresses from their environment, including, but not limited to, extreme temperature, salinity, drought, etc. These technologies enable improvements in the plant’s growth and development, as well as tolerance to abiotic stresses.

This Special Issue highlights the recent advancements in understanding the transcriptional regulation of genes regulating plants’ growth and development using CRISPR/cas9. We welcome all types of articles including research, methods, opinions, and reviews on CRISPR/cas9-based genome editing on the following aspects:

  • Regulation of gene transcription;
  • Abiotic stress;
  • Flowering time control;
  • Crop improvement;
  • Plant adaptation to climate change.

Studies using both model and non-model plant species are welcome.

Dr. Keh Chien Lee
Dr. Hoai Nguyen Nguyen
Guest Editors

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genome editing
  • CRISPR/cas9
  • regulation of gene transcription
  • plant adaptation to climate change
  • abiotic stress

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Published Papers (1 paper)

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Research

19 pages, 1959 KiB  
Article
A Protoplast System for CRISPR-Cas Ribonucleoprotein Delivery in Pinus taeda and Abies fraseri
by Barbara M. Marques, Daniel B. Sulis, Bethany Suarez, Chenmin Yang, Carlos Cofre-Vega, Robert D. Thomas, Justin G. A. Whitehill, Ross W. Whetten, Rodolphe Barrangou and Jack P. Wang
Plants 2025, 14(7), 996; https://doi.org/10.3390/plants14070996 - 22 Mar 2025
Viewed by 584
Abstract
Climate change profoundly impacts the health, productivity, and resilience of forest ecosystems and threatens the sustainability of forest products and wood-based industries. Innovations to enhance tree growth, development, and adaptation offer unprecedented opportunities to strengthen ecosystem resilience and mitigate the effects of climate [...] Read more.
Climate change profoundly impacts the health, productivity, and resilience of forest ecosystems and threatens the sustainability of forest products and wood-based industries. Innovations to enhance tree growth, development, and adaptation offer unprecedented opportunities to strengthen ecosystem resilience and mitigate the effects of climate change. Here, we established a method for protoplast isolation, purification, and CRISPR-Cas ribonucleoprotein (RNP) delivery in Pinus taeda and Abies fraseri as a step towards accelerating the genetic improvement of these coniferous tree species. In this system, purified protoplasts could be isolated from somatic embryos with up to 2 × 106 protoplasts/g of tissue and transfected with proteins and nucleotides, achieving delivery efficiencies up to 13.5%. The delivery of functional RNPs targeting phenylalanine ammonia lyase in P. taeda and phytoene desaturase in A. fraseri yielded gene editing efficiencies that reached 2.1% and 0.3%, respectively. This demonstration of RNP delivery for DNA-free genome editing in the protoplasts of P. taeda and A. fraseri illustrates the potential of CRISPR-Cas to enhance the traits of value in ecologically and economically important tree species. The editing system provides a foundation for future efforts to regenerate genome-edited forest trees to improve ecosystem health and natural resource sustainability. Full article
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