Molecular Characterization and Assessment of Insect Resistance of Transgenic Rice PAC-8
A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".
Deadline for manuscript submissions: 31 July 2026 | Viewed by 19
Special Issue Editor
2. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
Interests: genetic expression of plants in response to the environment; metabolic regulation; standardized cultivation and comprehensive utilization of medicinal plants
Special Issue Information
Dear Colleagues,
Insect resistance to Bacillus thuringiensis (Bt) crystal protein is a major threat to the long-term use of transgenic Bt crops. Gene stacking is a readily deployable strategy to delay the development of insect resistance while it may also broaden insecticidal spectrum. PAC-8 is a transgenic rice event created via Agrobacterium-mediated transformation for insect resistance and glufosinate tolerance by expressing Cry1Ab, Cry1Ca, and bar. A Southern blot analysis suggested that it is a single-copy T-DNA insertion event. Laboratory bioassays and field trials of multiple generations demonstrated that the transgenic rice event is highly active against major rice pests, including the striped stem borer (SSB, Chilo suppressalis) and the beet armyworm (BAW, Spodoptera exigua). The expression of the transgenes and the efficacy of insect resistance and glufosinate tolerance were stable over more than 5 generations. A field trial observed no significant difference in the basic agronomic traits between PAC-8 and the recipient rice.
Prof. Dr. Qiming Wang
Guest Editor
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Keywords
- transgenic rice
- Cry1Ab
- Cry1Ca
- insect resistance
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