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Plants
Special Issues
In Vitro Plant Regeneration and Biotechnological Approaches for Crop Improvement
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In Vitro Plant Regeneration and Biotechnological Approaches for Crop Improvement

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: 28 February 2026 | Viewed by 286

Special Issue Editor

Dr. Marga Pérez-Jiménez

E-Mail Website
Guest Editor
Instituto Murciano de Investigacion y Desarrollo Agrario y Alimentario, La Alberca, Spain
Interests: plant biotechnology; plant physiology; plant breeding

Special Issue Information

Dear Colleagues,

I am pleased to announce a forthcoming Special Issue in Plants, focusing on "In Vitro Plant Regeneration and Biotechnological Approaches for Crop Improvement". This issue will showcase cutting-edge research and comprehensive reviews, highlighting recent advances and innovative methodologies in plant tissue culture, genetic engineering, and relevant biotechnological strategies to enhance crop productivity, resilience, and quality.

Given the importance of in vitro technologies and biotechnological tools in modern plant science and agriculture, this Special Issue will provide a valuable platform for researchers to present novel findings, protocols, and perspectives on improving crop performance under diverse conditions.

Contributions, including original research articles, review papers, and short communications on (but not limited to) the following topics, are welcome for submission:

  • Somatic embryogenesis and organogenesis;
  • Genetic transformation and genome editing;
  • Micropropagation and synthetic seed technology;
  • Tissue culture-based stress tolerance studies;
  • Metabolite production through in vitro systems;
  • Advances in culture media and growth regulators.

If you or your collaborators are working on additional, relevant topics, we would welcome discussions to consider your work for submission. Feel free to share this opportunity within your networks as well.

For further details on submission guidelines and deadlines, please refer to the journal’s official website.

I look forward to receiving your valuable contributions.

Dr. Marga Pérez-Jiménez
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • agrobacterium
  • plant tissue culture
  • plant editing
  • organogenesis
  • embryogenesis

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (1 paper)

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Research

18 pages, 23218 KB  
Article
Induction of Embryogenic Callus, Protoplast Isolation, and PEG-Mediated Transformation Protocols in Eucommia ulmoides
by Hongrun Zhou, Zibo Zhou, Jiangyuan Zhang, Haoran Kan, Mengqi Yin, Han Zhang, Luyao Wang, Jie Zhao and Jing Ye
Plants 2026, 15(2), 194; https://doi.org/10.3390/plants15020194 - 8 Jan 2026
Viewed by 37
Abstract
Eucommia ulmoides, a tree species native to China, holds considerable medicinal, ecological, and industrial importance. However, the absence of an efficient and stable genetic transformation system poses significant challenges to gene function studies and molecular breeding in E. ulmoides. Protoplasts, which [...] Read more.
Eucommia ulmoides, a tree species native to China, holds considerable medicinal, ecological, and industrial importance. However, the absence of an efficient and stable genetic transformation system poses significant challenges to gene function studies and molecular breeding in E. ulmoides. Protoplasts, which lack cell walls, serve as effective receptors for transient transformation and are thus ideal for genetic engineering research. In this study, the optimal conditions for callus induction were identified, and formation of the embryogenic callus was confirmed by histological analysis. Furthermore, we developed an efficient protoplast isolation and PEG-mediated transient transformation system using suitable embryogenic callus as the starting material. Our findings revealed that the optimal medium for inducing embryogenic callus was B5 + 1.5 mg/L 6-BA + 0.5 mg/L NAA + 30 g/L sucrose + 7 g/L agar (pH = 5.8). In this medium, the induction rate of callus achieved 97.50%, and the rate of embryogenic callus formation was 86.30%. For protoplast isolation, the best conditions involved enzymatic digestion with 1.5% cellulase R-10 and 1.0% macerozyme R-10 at an osmotic pressure of 0.6 M for 4 h, resulting in 1.82 × 106 protoplasts/g FW with 91.13% viability. The highest transfection efficiency (53.23%) was attained when protoplasts were cultured with 10 µg of plasmid and 40% PEG4000 for 20 min. This study successfully established a stable and efficient system for protoplast isolation and transient transformation in E. ulmoides, offering technical support for exploring somatic hybridisation and transient gene expression in this species. Full article
(This article belongs to the Special Issue In Vitro Plant Regeneration and Biotechnological Approaches for Crop Improvement)
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