In Vitro Cell Models in Regenerative Medicine

A special issue of Pharmaceutics (ISSN 1999-4923). This special issue belongs to the section "Pharmaceutical Technology, Manufacturing and Devices".

Deadline for manuscript submissions: closed (30 June 2023) | Viewed by 5229

Special Issue Editors


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Guest Editor
1. Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, Niš, Serbia
2. Department for Cell and Tissue Engineering, Scientific Research Center for Biomedicine, Faculty of Medicine, University of Niš, Niš, Serbia
Interests: stem cells; tissue engineering; regenerative medicine; adipose tissue; macrophages; in vitro cell models; wound healing, examination of biological activity in cell models in vitro and animal models in vivo; molecular and human genetics
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
1. Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, 18000 Niš, Serbia
2. Department for Cell and Tissue Engineering, Scientific Research Center for Biomedicine, Faculty of Medicine, University of Niš, 18000 Niš, Serbia
Interests: skeletal tissues regeneration assisted by biomaterials and stem cells; biomaterials; tissue engineering models; regenerative medicine; examination of biological activity and toxicity of the various substances in animal models in vivo and cell models in vitro; macrophages; stem cells; cell and molecular biology; human genetics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are pleased to announce the new Special Issue for Pharmaceutics entitled "In Vitro Cell Models in Regenerative Medicine".

Due to the constant requirement for the reduction in animal use in research, the need for development of adequate alternative testing systems is rapidly arising. The most challenging step in the creation of an ideal in vitro system is mimicking the tissue environment in an appropriate way. Three-dimensional cell models are slowly overcoming traditional two-dimensional cultures, while organ-on-chip systems are introducing a real revolution in in vitro testing.

This Special Issue on "In Vitro Cell Models in Regenerative Medicine" encourages authors to submit their original research or review articles that bring new methods, solutions, and models for regenerative medicine and pharmaceutical purposes. Topics include but are not limited to biocompatibility testing in vitro, stem cells, 2D and 3D cell cultures, in vitro cell models for soft and hard tissue regeneration, in vitro cell models for testing drugs and medical devices for application in regenerative medicine, personalized in vitro cell models, matrices and scaffolds for 3D cell models, biomaterials, 3D printing, bioreactors, and pharmaceutics in regenerative medicine.

Dr. Sanja Stojanović
Prof. Dr. Stevo Najman
Guest Editors

Manuscript Submission Information

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Keywords

  • in vitro testing
  • in vitro cell models
  • cell culture methods
  • biocompatibility in vitro
  • stem cells
  • 2D and 3D cell models
  • bioreactors
  • organ-on-chip
  • biomaterials

Published Papers (2 papers)

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Research

12 pages, 3988 KiB  
Article
Isolation and Cultivation of Porcine Endothelial Cells, Pericytes and Astrocytes to Develop an In Vitro Blood–Brain Barrier Model for Drug Permeation Testing
by Verena Ledwig and Stephan Reichl
Pharmaceutics 2023, 15(6), 1688; https://doi.org/10.3390/pharmaceutics15061688 - 8 Jun 2023
Cited by 1 | Viewed by 1271
Abstract
The blood–brain barrier (BBB) is the bottleneck in the development of new drugs to reach the brain. Due to the BBB, toxic substances cannot enter the brain, but promising drug candidates also pass the BBB poorly. Suitable in vitro BBB models are therefore [...] Read more.
The blood–brain barrier (BBB) is the bottleneck in the development of new drugs to reach the brain. Due to the BBB, toxic substances cannot enter the brain, but promising drug candidates also pass the BBB poorly. Suitable in vitro BBB models are therefore of particular importance during the preclinical development process, as they can not only reduce animal testing but also enable new drugs to be developed more quickly. The aim of this study was to isolate cerebral endothelial cells, pericytes, and astrocytes from the porcine brain to produce a primary model of the BBB. Additionally, as primary cells are well suited by their properties but the isolation is complex and better reproducibility with immortalized cells must be ensured, there is a high demand for immortalized cells with suitable properties for use as a BBB model. Thus, isolated primary cells can also serve as the basis for a suitable immortalization technique to generate new cell lines. In this work, cerebral endothelial cells, pericytes, and astrocytes were successfully isolated and expanded using a mechanical/enzymatic method. Furthermore, in a triple coculture model, the cells showed a significant increase in barrier integrity compared with endothelial cell monoculture, as determined by transendothelial electrical resistance measurement and permeation studies using sodium fluorescein. The results demonstrate the opportunity to obtain all three cell types significantly involved in BBB formation from one species, thus providing a suitable tool for testing the permeation properties of new drug candidates. In addition, the protocols are a promising starting point to generate new cell lines of BBB-forming cells as a novel approach for BBB in vitro models. Full article
(This article belongs to the Special Issue In Vitro Cell Models in Regenerative Medicine)
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23 pages, 5603 KiB  
Article
Nano-Hydroxyapatite/PLGA Mixed Scaffolds as a Tool for Drug Development and to Study Metastatic Prostate Cancer in the Bone
by Annachiara Dozzo, Krishnakumar Chullipalliyalil, Michael McAuliffe, Caitriona M. O’Driscoll and Katie B. Ryan
Pharmaceutics 2023, 15(1), 242; https://doi.org/10.3390/pharmaceutics15010242 - 11 Jan 2023
Cited by 5 | Viewed by 3183
Abstract
(1) Background: Three-dimensional (3D) in vitro, biorelevant culture models that recapitulate cancer progression can help elucidate physio-pathological disease cues and enhance the screening of more effective therapies. Insufficient research has been conducted to generate in vitro 3D models to replicate the spread of [...] Read more.
(1) Background: Three-dimensional (3D) in vitro, biorelevant culture models that recapitulate cancer progression can help elucidate physio-pathological disease cues and enhance the screening of more effective therapies. Insufficient research has been conducted to generate in vitro 3D models to replicate the spread of prostate cancer to the bone, a key metastatic site of the disease, and to understand the interplay between the key cell players. In this study, we aim to investigate PLGA and nano-hydroxyapatite (nHA)/PLGA mixed scaffolds as a predictive preclinical tool to study metastatic prostate cancer (mPC) in the bone and reduce the gap that exists with traditional 2D cultures. (2) Methods: nHA/PLGA mixed scaffolds were produced by electrospraying, compacting, and foaming PLGA polymer microparticles, +/− nano-hydroxyapatite (nHA), and a salt porogen to produce 3D, porous scaffolds. Physicochemical scaffold characterisation together with an evaluation of osteoblastic (hFOB 1.19) and mPC (PC-3) cell behaviour (RT-qPCR, viability, and differentiation) in mono- and co-culture, was undertaken. (3) Results: The results show that the addition of nHA, particularly at the higher-level impacted scaffolds in terms of mechanical and degradation behaviour. The nHA 4 mg resulted in weaker scaffolds, but cell viability increased. Qualitatively, fluorescent imaging of cultures showed an increase in PC-3 cells compared to osteoblasts despite lower initial PC-3 seeding densities. Osteoblast monocultures, in general, caused an upregulation (or at least equivalent to controls) in gene production, which was highest in plain scaffolds and decreased with increases in nHA. Additionally, the genes were downregulated in PC3 and co-cultures. Further, drug toxicity tests demonstrated a significant effect in 2D and 3D co-cultures. (4) Conclusions: The results demonstrate that culture conditions and environment (2D versus 3D, monoculture versus co-culture) and scaffold composition all impact cell behaviour and model development. Full article
(This article belongs to the Special Issue In Vitro Cell Models in Regenerative Medicine)
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