Nucleic-Acid-Based Strategies and Nanotechnology Applications for Targeted Therapy, 2nd Edition

A special issue of Pharmaceutics (ISSN 1999-4923). This special issue belongs to the section "Drug Targeting and Design".

Deadline for manuscript submissions: 31 March 2026 | Viewed by 630

Special Issue Editor


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Guest Editor
Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy
Interests: peptide nucleic acid; G-quadruplex; delivery; solid-phase synthesis; small molecule-DNA interactions
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Special Issue Information

Dear Colleagues,

Due to the recent attention and growing potential that nucleic acids have gained, together with the demand for improving current controlled-release systems, we are pleased to announce this Special Issue, entitled “Nucleic-Acid-Based Strategies and Nanotechnology Applications for Targeted Therapy, 2nd Edition”. The purpose of this Special Issue is to collate papers reporting on, but not limited to, oligonucleotide synthesis, biochemical and biophysical characterization of nucleic acids, new oligonucleotide-based delivery systems, and biomaterial and polymer development. Articles embracing originality and novelty are welcome to be submitted to this Special Issue. We look forward to receiving your contributions.

Dr. Andrea Patrizia Falanga
Guest Editor

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Keywords

  • nucleic acids
  • drug delivery
  • nanomedicine
  • biotechnology
  • material science

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Published Papers (1 paper)

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Research

22 pages, 4984 KB  
Article
The Aptamer bi-(AID-1-T) Synergizes with Radiation to Inhibit Proliferation of Human Glioma Cells
by Svetlana Pavlova, Ksenia Rubetskaya, Lika Fab, Ekaterina Savchenko, Nadezhda Samoylenkova, Alexander Revishchin, Anastasia Ryabova, Natalia Antipina, Mikhail Galkin, Andrey Golanov, Dmitry Usachev, Alexey Kopylov and Galina Pavlova
Pharmaceutics 2025, 17(11), 1442; https://doi.org/10.3390/pharmaceutics17111442 - 8 Nov 2025
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Abstract
Background: High-grade gliomas are treatment-resistant and prone to aggressive recurrence. Although radiation therapy is a fundamental treatment, it often fails to eradicate tumors and can enhance the migratory potential of surviving cells, promoting relapse. Anti-proliferative aptamers are novel agents that show promise, but [...] Read more.
Background: High-grade gliomas are treatment-resistant and prone to aggressive recurrence. Although radiation therapy is a fundamental treatment, it often fails to eradicate tumors and can enhance the migratory potential of surviving cells, promoting relapse. Anti-proliferative aptamers are novel agents that show promise, but their combination with radiation therapy and their effects on invasive phenotypes require further investigation. Objectives: This study evaluated the effects of ionizing radiation on the viability and migration of human glioma cells, both alone and in combination with the anti-proliferative aptamer bi-(AID-1-T). The study aimed to determine whether the aptamer could enhance the efficacy of radiotherapy and counteract ionizing radiation-induced pro-migratory effects. Methods: The study was conducted on cell cultures of primary and relapsed human glioma. The effects of combined radiation (single dose of 20 Gy) and the bi-(AID-1-T) aptamer (10 μM) were assessed using the MTS assay, Transwell analysis, immunocytochemistry and transcriptome analysis. Results: Ionizing radiation alone reduced proliferation in primary gliomas, but increased proliferation in recurrent cultures. Ionizing radiation also increased migration in both types of gliomas. Combining ionizing radiation with the bi-(AID-1-T) aptamer produced a synergistic effect: it significantly reduced cell proliferation and migration, and suppressed the ionizing radiation-induced migratory enhancement, more effectively than either treatment alone. Transcriptome analysis revealed that combination treatment decreased the expression of pro-proliferative and migratory genes (e.g., PDPN, CDH3), while increasing the expression of anti-migratory (RND3) and pro-apoptotic genes (e.g., XAF1, SEMA3A). Thus, combination treatment significantly reduces tumor cell proliferation and migration; however, further studies on surviving cells are needed. Full article
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