Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
5.1
3.7
Journals
Pathogens
Special Issues
Diagnostics and Surveillance of Arboviral Diseases
share announcement

Diagnostics and Surveillance of Arboviral Diseases

A special issue of Pathogens (ISSN 2076-0817).

Deadline for manuscript submissions: closed (20 March 2022) | Viewed by 18292

Special Issue Editors

Prof. Dr. Jose Mauro Peralta

E-Mail Website
Guest Editor
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
Interests: molecular and immunological diagnosis of infectious diseases
Prof. Pedro Fernando da Costa Vasconcelos

E-Mail Website
Guest Editor
Universidade do Estado do Pará, Belém, Pará, Brazil and Instituto Evandro Chagas, Ananindeua, Pará, Brazil
Interests: diagnostic; genetic characterization; immunology and pathogenesis of arboviruses

Special Issue Information

Dear Colleagues

Arboviruses are a diverse group of viruses that are transmitted by bites of hematophagous insects to humans, many of which can cause diseases associated with significant human morbidity and mortality worldwide, while some are apparently restricted to certain geographic areas. Throughout history, infections caused by arboviruses have resulted in illness or death in large numbers of people, as well as livestock, resulting in huge economic losses. In tropical areas, they have been considered important neglected diseases and a long‐term public health problem. Over the last 30 years, the emergence and/or resurgence of arboviruses have posed an additional considerable global health threat and they are globally distributed on all continents except Antarctica. Fever accompanied by hemorrhagic events are clinical manifestations commonly observed in several arbovirus diseases, including Dengue virus (DENV), Chikungunya virus (CHIKV), and Yellow fever virus (YFV), while others cause viral encephalitis, such as West Nile virus (WNV), Japanese encephalitis virus (JEV), tick‐borne encephalitis virus (TBEV), and Zika virus (ZIKV). As arboviruses have natural foci, they cannot be eliminated and will persist in nature. Therefore, the surveillance of arboviruses, their respective transmission vectors, and related diseases are of pivotal importance for the prevention and control of these viral diseases. Currently, ZIKV has been widespread in the Americas since 2015, while DENV and CHIKV have already become firmly established in most tropical and many non-tropical regions for decades. These three arboviral diseases can overlap in their endemic areas and cause similar clinical pictures, especially in the initial stages of disease. In addition, the occurrence of neurological manifestations has increasingly been described in association with these infections. Atypical neurological complications have been reported, such as acute disseminated encephalomyelitis, optic neuromyelitis, and Guillain-Barré syndrome, the latter with few or no signs of DENV and ZIKV infection before the neurological findings. Encephalitis has been the most common neurological manifestation associated with DENV infection. These clinical complications and previously unknown severe outcomes call for more accurate tools for the precise diagnosis, distinction, and characterization of these and other arboviruses.

In this Special Issue, we invite the arboviral diseases community to submit research articles, reviews, or short communications highlighting critical advancements in our understanding of diagnostic approaches and surveillance of arboviruses. The development of new assays that can discriminate between different arboviral infections is of particular interest. Timely and accurate diagnosis is crucial for patient management, prevention of unnecessary therapies, rapid adoption of vector control measures, and collection of epidemiological data. Finally, considering the importance and global impact of arboviruses as public health threats and the emergence/re-emergence of such viruses we look forward to your submissions. 

Prof. Dr. Jose Mauro Peralta
Prof. Pedro Fernando da Costa Vasconcelos
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Arboviruses Arboviral co-infections
  • Diagnostic approaches
  • Epidemiology and surveillance
  • Molecular epidemiology
  • New diagnostic targets
  • Prevention and vaccine candidates

Published Papers (6 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review, Other

16 pages, 1833 KiB  
Article
Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil
by Thiara Manuele Alves de Souza, Raquel Curtinhas de Lima, Victor Edgar Fiestas Solórzano, Paulo Vieira Damasco, Luiz José de Souza, Juan Camilo Sanchez-Arcila, Gabriel Macedo Costa Guimarães, Iury Amâncio Paiva, Monique da Rocha Queiroz Lima, Fernanda de Bruycker-Nogueira, Larissa Cristina Teixeira Tomé, Mariana Rosa Inácio Coelho, Sandro Patroca da Silva, Luzia Maria de Oliveira-Pinto, Elzinandes Leal de Azeredo and Flavia Barreto dos Santos
Pathogens 2022, 11(2), 245; https://doi.org/10.3390/pathogens11020245 - 14 Feb 2022
Cited by 2 | Viewed by 2054
Abstract
The co-circulation of chikungunya virus (CHIKV), dengue virus (DENV) and Zika virus (ZIKV) in Rio de Janeiro (RJ), Brazil, caused a challenging triple epidemic, as they share similar clinical signs and symptoms and geographical distribution. Here, we aimed to investigate the clinical and [...] Read more.
The co-circulation of chikungunya virus (CHIKV), dengue virus (DENV) and Zika virus (ZIKV) in Rio de Janeiro (RJ), Brazil, caused a challenging triple epidemic, as they share similar clinical signs and symptoms and geographical distribution. Here, we aimed to investigate the clinical and laboratorial aspects of chikungunya suspected cases assisted in RJ during the 2018 outbreak, focusing on the differential diagnosis with dengue and zika. All suspected cases were submitted to molecular and/or serological differential diagnostic approaches to arboviruses. A total of 242 cases suspected of arbovirus infection were investigated and 73.6% (178/242) were molecular and/or serologically confirmed as chikungunya. In RT-qPCR confirmed cases, cycle threshold (Ct) values ranged from 15.46 to 35.13, with acute cases presenting lower values. Chikungunya cases were mainly in females (64%) and the most frequently affected age group was adults between 46 to 59 years old (27%). Polyarthralgia affected 89% of patients, especially in hands and feet. No dengue virus (DENV) and Zika virus (ZIKV) infections were confirmed by molecular diagnosis, but 9.5% (23/242) had serological evidence of DENV exposure by the detection of specific anti-DENV IgM or NS1, and 42.7% (76/178) of chikungunya positive cases also presented recent DENV exposure reflected by a positive anti-DENV IgM or NS1 result. A significantly higher frequency of arthritis (p = 0.023) and limb edema (p < 0.001) was found on patients with CHIKV monoinfection compared to dengue patients and patients exposed to both viruses. Lastly, phylogenetic analysis showed that the chikungunya cases were caused by the ECSA genotype. Despite the triple arboviruses’ epidemic in the state of RJ, most patients with fever and arthralgia investigated here were diagnosed as chikungunya cases, and the incidence of CHIKV/DENV co-detection was higher than that reported in other studies. Full article
(This article belongs to the Special Issue Diagnostics and Surveillance of Arboviral Diseases)
►▼ Show Figures

Figure 1

10 pages, 1075 KiB  
Article
Neopterin and CXCL-10 in Cerebrospinal Fluid as Potential Biomarkers of Neuroinvasive Dengue and Chikungunya
by Marzia Puccioni-Sohler, Samya J. da Silva, Luiz C. S. Faria, David C. B. I. Cabral and Mauro J. Cabral-Castro
Pathogens 2021, 10(12), 1626; https://doi.org/10.3390/pathogens10121626 - 15 Dec 2021
Cited by 2 | Viewed by 2484
Abstract
Dengue (DENV) and chikungunya viruses (CHIKV) cause severe neurological complications, sometimes undiagnosed. Therefore, the use of more accessible neuroinflammatory biomarkers can be advantageous considering their diagnostic and prognostic potential for aggravated clinical outcomes. In this study, we aimed to evaluate neopterin and C-X-C [...] Read more.
Dengue (DENV) and chikungunya viruses (CHIKV) cause severe neurological complications, sometimes undiagnosed. Therefore, the use of more accessible neuroinflammatory biomarkers can be advantageous considering their diagnostic and prognostic potential for aggravated clinical outcomes. In this study, we aimed to evaluate neopterin and C-X-C motif chemokine ligand 10 (CXCL-10) in cerebrospinal fluid (CSF) for the diagnosis of neuroinvasive DENV and CHIKV. We analyzed the CSF of 66 patients with neurological disorders, comprising 12 neuroinvasive DENV/CHIKV, 20 inflammatory control (viral, bacterial, and fungal meningitis, and autoimmune disorders), and 24 noninflammatory control (cerebrovascular disease, dementia, neoplasm). There was no difference between the concentration of CSF neopterin in the neuroinvasive DENV/CHIKV and control groups. However, there was a significant difference in the CXCL-10 level when comparing the neuroinvasive DENV/CHIKV group and the non-inflammatory control (p < 0.05). Furthermore, we found a linear correlation between neopterin and CXCL-10 CSF levels in the three groups. For the DENV/CHIKV neuroinvasive diagnosis, the ROC curve showed the best cut-off values for CSF neopterin at 11.23 nmol/L (sensitivity of 67% and specificity of 63%), and for CSF CXCL-10 at 156.5 pg/mL (91.7% sensitivity and specificity). These results show that CXCL-10 in CSF represents an accurate neuroinflammatory biomarker that may contribute to neuroinvasive DENV/CHIKV diagnosis. Full article
(This article belongs to the Special Issue Diagnostics and Surveillance of Arboviral Diseases)
►▼ Show Figures

Figure 1

11 pages, 1298 KiB  
Article
Reporter Virus Neutralization Test Evaluation for Dengue and Zika Virus Diagnosis in Flavivirus Endemic Area
by Jannyce G. C. Nunes, Bruno T. D. Nunes, Chao Shan, Adriana F. Moraes, Tais R. Silva, Maria H. R. de Mendonça, Liliane L. das Chagas, Franco A. e Silva, Raimunda S. S. Azevedo, Eliana V. P. da Silva, Livia C. Martins, Jannifer O. Chiang, Livia M. N. Casseb, Daniele F. Henriques, Pedro F. C. Vasconcelos, Rommel M. R. Burbano, Pei-Yong Shi and Daniele B. A. Medeiros
Pathogens 2021, 10(7), 840; https://doi.org/10.3390/pathogens10070840 - 3 Jul 2021
Cited by 3 | Viewed by 3327
Abstract
Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple [...] Read more.
Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple flavivirus. Here, we evaluate the performance of ZIKV and DENV Rluc RVNT and ZIKV mCh RVNT assays in comparison to the conventional PRNT assay against patient sera collected before and during ZIKV outbreak in Brazil. These samples were categorized into groups based on (1) acute and convalescent samples according to the time of disease, and (2) laboratorial diagnostic results (DENV and ZIKV RT-PCR and IgM-capture ELISA). Our results showed that DENV Rluc assay presented 100% and 78.3% sensitivity and specificity, respectively, with 93.3% accuracy, a similar performance to the traditional PRNT. ZIKV RVNT90, on the other hand, showed much better ZIKV antibody detection performance (around nine-fold higher) when compared to PRNT, with 88% clinical sensitivity. Specificity values were on average 76.8%. Even with these results, however, ZIKV RVNT90 alone was not able to reach a final diagnostic conclusion for secondary infection in human samples due to flavivirus cross reaction. As such, in regions where the flavivirus differential diagnosis represents a challenge, we suggest the establishment of a RVNT panel including other flaviviruses circulating in the region, associated with the other serological techniques such as IgM ELISA and the investigation of seroconversion, in order to help define an accurate diagnostic conclusion using serology. Full article
(This article belongs to the Special Issue Diagnostics and Surveillance of Arboviral Diseases)
►▼ Show Figures

Figure 1

16 pages, 1980 KiB  
Article
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors
by Alice L. N. Queiroz, Rafael S. Barros, Sandro P. Silva, Daniela S. G. Rodrigues, Ana C. R. Cruz, Flávia B. dos Santos, Pedro F. C. Vasconcelos, Robert B. Tesh, Bruno T. D. Nunes and Daniele B. A. Medeiros
Pathogens 2021, 10(6), 693; https://doi.org/10.3390/pathogens10060693 - 3 Jun 2021
Cited by 5 | Viewed by 2879
Abstract
From 2016 to 2018, Brazil faced the biggest yellow fever (YF) outbreak in the last 80 years, representing a risk of YF reurbanization, especially in megacities. Along with this challenge, the mass administration of the fractionated YF vaccine dose in a naïve population [...] Read more.
From 2016 to 2018, Brazil faced the biggest yellow fever (YF) outbreak in the last 80 years, representing a risk of YF reurbanization, especially in megacities. Along with this challenge, the mass administration of the fractionated YF vaccine dose in a naïve population brought another concern: the possibility to increase YF adverse events associated with viscerotropic (YEL-AVD) or neurological disease (YEL-AND). For this reason, we developed a quantitative real time RT-PCR (RT-qPCR) assay based on a duplex TaqMan protocol to distinguish broad-spectrum infections caused by wild-type yellow fever virus (YFV) strain from adverse events following immunization (AEFI) by 17DD strain during the vaccination campaign used to contain this outbreak. A rapid and more accurate RT-qPCR assay to diagnose YFV was established, being able to detect even different YFV genotypes and geographic strains that circulate in Central and South America. Moreover, after testing around 1400 samples from human cases, non-human primates and mosquitoes, we detected just two YEL-AVD cases, confirmed by sequencing, during the massive vaccination in Brazilian Southeast region, showing lower incidence than AEFI as expected. Full article
(This article belongs to the Special Issue Diagnostics and Surveillance of Arboviral Diseases)
►▼ Show Figures

Figure 1

Review

Jump to: Research, Other

17 pages, 3608 KiB  
Review
Nanoparticles as Vaccines to Prevent Arbovirus Infection: A Long Road Ahead
by Gabriel Augusto Pires de Souza, Raíssa Prado Rocha, Ricardo Lemes Gonçalves, Cyntia Silva Ferreira, Breno de Mello Silva, Renato Fróes Goulart de Castro, João Francisco Vitório Rodrigues, João Carlos Vilela Vieira Júnior, Luiz Cosme Cotta Malaquias, Jônatas Santos Abrahão and Luiz Felipe Leomil Coelho
Pathogens 2021, 10(1), 36; https://doi.org/10.3390/pathogens10010036 - 5 Jan 2021
Cited by 13 | Viewed by 3882
Abstract
Arthropod-borne viruses (arboviruses) are a significant public health problem worldwide. Vaccination is considered one of the most effective ways to control arbovirus diseases in the human population. Nanoparticles have been widely explored as new vaccine platforms. Although nanoparticles’ potential to act as new [...] Read more.
Arthropod-borne viruses (arboviruses) are a significant public health problem worldwide. Vaccination is considered one of the most effective ways to control arbovirus diseases in the human population. Nanoparticles have been widely explored as new vaccine platforms. Although nanoparticles’ potential to act as new vaccines against infectious diseases has been identified, nanotechnology’s impact on developing new vaccines to prevent arboviruses is unclear. Thus, we used a comprehensive bibliographic survey to integrate data concerning the use of diverse nanoparticles as vaccines against medically important arboviruses. Our analysis showed that considerable research had been conducted to develop and evaluate nanovaccines against Chikungunya virus, Dengue virus, Zika virus, Japanese encephalitis virus, and West Nile virus. The main findings indicate that nanoparticles have great potential for use as a new vaccine system against arboviruses. Most of the studies showed an increase in neutralizing antibody production after mouse immunization. Nevertheless, even with significant advances in this field, further efforts are necessary to address the nanoparticles’ potential to act as a vaccine against these arboviruses. To promote advances in the field, we proposed a roadmap to help researchers better characterize and evaluate nanovaccines against medically important arboviruses. Full article
(This article belongs to the Special Issue Diagnostics and Surveillance of Arboviral Diseases)
►▼ Show Figures

Figure 1

Other

Jump to: Research, Review

12 pages, 627 KiB  
Brief Report
Co-Circulation of Two Independent Clades and Persistence of CHIKV-ECSA Genotype during Epidemic Waves in Rio de Janeiro, Southeast Brazil
by Allison Araújo Fabri, Cintia Damasceno dos Santos Rodrigues, Carolina Cardoso dos Santos, Flávia Löwen Levy Chalhoub, Simone Alves Sampaio, Nieli Rodrigues da Costa Faria, Maria Celeste Torres, Vagner Fonseca, Patricia Brasil, Guilherme Calvet, Luiz Carlos Junior Alcantara, Ana Maria Bispo de Filippis, Marta Giovanetti and Fernanda de Bruycker-Nogueira
Pathogens 2020, 9(12), 984; https://doi.org/10.3390/pathogens9120984 - 26 Nov 2020
Cited by 13 | Viewed by 2351
Abstract
The Chikungunya virus infection in Brazil has raised several concerns due to the rapid dissemination of the virus and its association with several clinical complications. Nevertheless, there is limited information about the genomic epidemiology of CHIKV circulating in Brazil from surveillance studies. Thus, [...] Read more.
The Chikungunya virus infection in Brazil has raised several concerns due to the rapid dissemination of the virus and its association with several clinical complications. Nevertheless, there is limited information about the genomic epidemiology of CHIKV circulating in Brazil from surveillance studies. Thus, to better understand its dispersion dynamics in Rio de Janeiro (RJ), one of the most affected states during the 2016–2019 epidemic waves, we generated 23 near-complete genomes of CHIKV isolates from two main cities located in the metropolitan mesoregion, obtained directly from clinical samples. Our phylogenetic reconstructions suggest the 2019-CHIKV-ECSA epidemic in RJ state was characterized by the co-circulation of multiple clade (clade A and B), highlighting that two independent introduction events of CHIKV-ECSA into RJ state have occurred between 2016–2019, both mediated from the northeastern region. Interestingly, we identified that the two-clade displaying eighteen characteristic amino acids changes among structural and non-structural proteins. Our findings reinforce that genomic data can provide information about virus genetic diversity and transmission dynamics, which might assist in the arbovirus epidemics establishing of an effective surveillance framework. Full article
(This article belongs to the Special Issue Diagnostics and Surveillance of Arboviral Diseases)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-6
Back to TopTop