Drug Resistant Pathogens: Diagnosis, Treatment, and Global Health Implications

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Bacterial Pathogens".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 420

Special Issue Editors


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Guest Editor
Infectious Disease Unit, ARNAS Civico-Di Cristina Hospital, Piazza Leotta, 5, Palermo, Italy
Interests: sepsis; critical illness; antimicrobial stewardship
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Special Issue Information

Dear Colleagues,

this Special Issue, "Drug Resistant Pathogens: Diagnosis, Treatment, and Global Health Implications", will highlight the pressing threat posed by drug-resistant microorganisms to global health. Antimicrobial abuse and misuse have led to an increase in drug resistance in bacteria, viruses, fungi, and parasites. This Special Issue will investigate novel treatment approaches to combating resistances, as well as diagnostics for the early and precise identification of resistant strains. This Special Issue will consider the intricacy of resistance mechanisms, including hereditary and environmental components, and their consequences for public health policy and therapeutic procedures. The socioeconomic cost of medication resistance will also be discussed, with an emphasis on how differences in healthcare infrastructure and resource availability can worsen the problem's effects worldwide. We must aim to maintain the effectiveness of current therapies and encourage the sustainable use of antibiotics globally by conducting interdisciplinary research.

Dr. Giuseppe Pipitone
Dr. Alberto Enrico Maraolo
Dr. Luigi Principe
Guest Editors

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Keywords

  • antibiotics
  • multi-drug-resistant microorganisms
  • difficult-to-treat infections

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Published Papers (1 paper)

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Research

14 pages, 2554 KiB  
Article
Antibacterial Evaluation of Tricyclic Antidepressants Against S. aureus and the Possible Pathways of the Mechanism of Action
by Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Lívia Gurgel do Amaral Valente Sá, Lara Elloyse Almeida Moreira, Cecília Rocha da Silva, João Batista de Andrade Neto, Érica Rayanne Mota da Costa, Thais Lima Ferreira, Leilson Carvalho de Oliveira, Beatriz Oliveira de Souza, Dávylla Rênnia Saldanha Pinheiro, Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Manoel Odorico de Moraes and Hélio Vitoriano Nobre Júnior
Pathogens 2025, 14(7), 613; https://doi.org/10.3390/pathogens14070613 - 20 Jun 2025
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Abstract
The resistance of Staphylococcus aureus to conventional pharmacological treatments has gradually increased. Thus, new therapeutic strategies are needed. Three tricyclic antidepressants (TCAs), amitriptyline (AMT), nortriptyline (NOR), and clomipramine (CLO), stand out with potential in this regard. Thus, the objective of this study was [...] Read more.
The resistance of Staphylococcus aureus to conventional pharmacological treatments has gradually increased. Thus, new therapeutic strategies are needed. Three tricyclic antidepressants (TCAs), amitriptyline (AMT), nortriptyline (NOR), and clomipramine (CLO), stand out with potential in this regard. Thus, the objective of this study was to evaluate the antibacterial activity of TCAs against S. aureus. The methodology used broth microdilution, checkerboard, flow cytometry, fluorescence microscopy, and scanning electron microscopy (SEM) techniques. The results showed that the minimum inhibitory concentration (MIC) of AMT was 256 µg/mL, while the MIC of NOR was 128 µg/mL, and the MIC of CLO was between 64 and 128 µg/mL. The TCAs exhibited bactericidal activity. In the analysis of the association with oxacillin (OXA), AMT exhibited 75% synergism, while NOR and CLO obtained 62.5%. In combination with vancomycin (VAN), AMT and NOR presented 100% additive interactions, while CLO exhibited 62.5% indifferent interactions. The mechanism of TCAs, isolated and combined with OXA, was associated with a reduction in cell viability, resulting from their action on the bacterial genetic material and generation of oxidative stress. Furthermore, the action of the drugs produced intense morphological changes in the bacterial cells. In conclusion, TCAs are a potential alternative for antistaphylococcal therapy. Full article
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