Special Issue "Phage Therapy in Aquaculture: Current Developments and Future Challenges"

A special issue of Pathogens (ISSN 2076-0817).

Deadline for manuscript submissions: 30 December 2020.

Special Issue Editors

Dr. Pantelis Katharios
Website1 Website2
Guest Editor
1. Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Greece
2. Aquatic Biologicals
Interests: fish diseases; phage microbiology and phage therapy; vaccine development; disease prevention in aquaculture
Prof. Roberto Bastías
Guest Editor
Pontificia Universidad Católica de Valparaíso, Chile
Interests: Marine Microbiology,Bacteriophages

Special Issue Information

Dear Colleagues,

Aquaculture is the fastest growing sector of animal production worldwide. Very soon, most fishery products will come from aquaculture than from traditional fisheries. Intensification of aquaculture leads to an increase of disease outbreaks. When it comes to bacterial infections, administration of antibiotics is the first line of defense for the fish producer. Rational use of antibiotics is common practice in all animal production sectors; nevertheless, the emergence of antimicrobial resistance as one of the most significant threats for humanity has created an urgent need to replace antibiotics with more sustainable, alternative methods. Phage therapy is one of the most promising alternatives, and there is a steadily increasing volume of scientific publications towards this direction.

Phage research for aquaculture applications is currently being practiced in several parts of the world, including Europe, Asia, America, and Oceania. New phages with potency against fish and shellfish pathogens are continuously described, and new information is added to the scientific literature every day. However, there are many gaps in significant aspects before we can see the successful transition of phage therapy from the lab to the actual aquaculture production. These include stability of phages in the aquaculture environment, resistance development, upscaling of production, quality of the product, integration of modern approaches such as phage engineering, and of course regulation of phage therapy.

These are the main issues that we aspire to cover through this Special Issue of the journal Pathogens. The scope of the issue also includes current developments in phage research in relation to aquaculture (e.g., the role of prophages in virulence of aquaculture pathogens), discovery of new phages against aquaculture pathogens, and trials and applications in live feeds, fish, and invertebrates. We would like to encourage the submission of papers dealing with the use of phage lytic enzymes, the use of phages as probiotics or in combination with probiotics, and the role of phages in the intestinal environment of fish. 

Both original research and review articles are welcomed.

Dr. Pantelis Katharios
Prof. Roberto Bastías
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • phage microbiology
  • aquaculture
  • phage therapy
  • antibiotic resistance
  • phage genomics

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:


Open AccessArticle
Isolation, Characterization, and Application of a Bacteriophage Infecting the Fish Pathogen Aeromonas hydrophila
Pathogens 2020, 9(3), 215; https://doi.org/10.3390/pathogens9030215 - 13 Mar 2020
Bacteriophages are increasingly being used as biological control agents against pathogenic bacteria. In the present study, we isolate and characterize bacteriophage Akh-2 from Geoje Island, South Korea, to evaluate its utility in controlling motile Aeromonas septicemia. Akh-2 lysed four of the seven Aeromonas [...] Read more.
Bacteriophages are increasingly being used as biological control agents against pathogenic bacteria. In the present study, we isolate and characterize bacteriophage Akh-2 from Geoje Island, South Korea, to evaluate its utility in controlling motile Aeromonas septicemia. Akh-2 lysed four of the seven Aeromonas hydrophila strains tested. Transmission electron microscopy analysis showed that Akh-2 belongs to the Siphoviridae family, with head and tail sizes of 50 ± 5 and 170 ± 5 nm, respectively. One-step growth curve analysis revealed that the phage has a latent period of 50 ± 5 min and a burst size of 139 ± 5 plaque-forming units per infected cell. The phage appeared stable in a pH range of 6–8 and a temperature range of −80 to 46 °C. Based on next-generation sequencing analysis, its genome is 114,901 bp in size, with a 44.22% G + C content and 254 open reading frames. During an artificial induction of the disease, loach (Misgurnus anguillicaudatus) treated with Akh-2 showed an increased survival rate and time compared with the non-treated control. Our results suggest that Akh-2 is a potential biological agent for the treatment of Aeromonas infections in fish. Full article
Show Figures

Figure 1

Back to TopTop