Beneficial Microorganisms Against Phytopathogens: Innovations in Sustainable Plant Protection

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Plant Microbe Interactions".

Deadline for manuscript submissions: 28 February 2026 | Viewed by 165

Special Issue Editors


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Guest Editor
Research Unit Induced Resistance and Plant Bioprotection, University of Reims, EA 4707 USC INRAe 1488, SFR Condorcet FR CNRS 3417, 51100 Reims, France
Interests: plant-microbe interaction; stress physiology plants responses to biotic and abiotic stress; crop protection; biological control
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Special Issue Information

Dear Colleagues,

We are pleased to invite you to contribute to this Special Issue, entitled "Beneficial Microorganisms Against Phytopathogens: Innovations in Sustainable Plant Protection". The urgent need reduce the usage of chemical pesticides, tackle emerging pathogen resistance, and ensure environmentally sustainable crop production has highlighted the vital role that beneficial microorganisms play in the management of plant disease. These microorganisms, such as plant growth-promoting endophytic and rhizobacteria (PGPR) and free-living and mycorrhizal fungi, offer diverse mechanisms of biocontrol; these include pathogen suppression, competition, the induction of systemic resistance, and the promotion of plant growth. Exploring these interactions is crucial for building resilient agroecosystems and advancing eco-friendly disease control strategies.

This Special Issue aims to provide a platform for the latest scientific advances regarding the identification, characterization, and application of beneficial microorganisms to combat phytopathogens. The scope of this Special Issue aligns with the journal's mission to support innovations in plant science, microbiology, biotechnology, and sustainable agriculture. We are particularly interested in multidisciplinary studies that provide molecular, ecological, and applied perspectives to uncover how microbial communities can be integrated or engineered for targeted, effective, and scalable biocontrol solutions.

This Special Issue welcomes the submission of original research articles and comprehensive reviews whose scope includes, but is not limited to, the following topics:

  • Mechanisms of microbial antagonism and induced resistance;
  • Host–microbe–pathogen interactions and plant immune modulation;
  • Formulation and delivery technologies for microbial inoculants;
  • Microbiome dynamics and predictive modeling for biocontrol;
  • Microbial adaptation, colonization dynamics, and microbiome interactions under field conditions, including predictive modeling for biocontrol efficacy across cropping systems;
  • Regulatory and commercialization perspectives for microbial-based products.

We look forward to receiving your contributions and building a high-impact collection that advances the field of plant–microbe interactions and sustainable plant protection.

Prof. Dr. Essaid Ait Barka
Prof. Dr. Rachid Lahlali
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • beneficial microorganisms
  • biocontrol
  • phytopathogens
  • PGPR
  • endophytes
  • mycorrhizal fungi
  • induced systemic resistance
  • microbiome engineering
  • synthetic microbial communities
  • sustainable agriculture

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Published Papers (1 paper)

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Research

13 pages, 280 KB  
Article
Entomopathogenic Nematodes and Bioactive Compounds of Their Bacterial Endosymbionts Act Synergistically in Combination with Spinosad to Kill Phthorimaea operculella (Zeller, 1873) (Lepidoptera: Gelechiidae), a Serious Threat to Food Security
by Ebubekir Yüksel, Rachid Lahlali, Aydemir Barış, Muhammad Sameeullah, Furkan Ulaş, Abdurrahman Sami Koca, Essaid Ait Barka, Mustafa İmren and Abdelfattah Dababat
Microorganisms 2025, 13(10), 2368; https://doi.org/10.3390/microorganisms13102368 - 15 Oct 2025
Abstract
As a staple food, potato (Solanum tuberosum L.) (Solanaceae) is one of the most produced food crops to ensure food security. The potato tuber moth (PTM), Phthorimaea operculella (Zeller, 1873) (Lepidoptera: Gelechiidae), is a major pest of potato, damaging both the growing [...] Read more.
As a staple food, potato (Solanum tuberosum L.) (Solanaceae) is one of the most produced food crops to ensure food security. The potato tuber moth (PTM), Phthorimaea operculella (Zeller, 1873) (Lepidoptera: Gelechiidae), is a major pest of potato, damaging both the growing and storage processes. In recent years, green pest control strategies have been gaining importance to reduce the adverse effects of chemicals and protect the environment. Entomopathogenic nematodes (EPNs) and their bacterial endosymbionts (Xenorhabdus and Photorhabdus spp.) have been one of the top topics studied in sustainable pest control approaches. In the present study, the two most common EPN species, Steinernema feltiae and Heterorhabditis bacteriophora, and their bacterial associates, Xenorhabdus bovienii and Photorhabdus luminescens subsp. kayaii were evaluated against PTM larvae separately and in combination with spinosad. The survival rates of infective juveniles (IJs) of EPNs were over 92% after 72 h of direct exposure to spinosad. Co-application of EPNs and bioactive compounds (BACs) of endosymbiotic bacteria with spinosad induced synergistic interactions and achieved the maximum mortality (100%) in PTM larvae 48 h post-treatment. Spinosad and BAC combinations were highly efficient in controlling the PTM larvae and provided LT50 values below 23.0 h. Gas chromatography mass spectrometry (GC-MS) analysis identified 29 compounds in total, 20 of which belonged to P. luminescens subsp. kayaii. The results indicate that the integration of EPNs and BACs of endosymbiotic bacteria with spinosad presents a synergistic interaction and enhances pest control efficacy. Full article
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