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Microorganisms
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Microbial Biotechnology: The Biodiversity, Properties and Benefits of Microorganisms in...
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Microbial Biotechnology: The Biodiversity, Properties and Benefits of Microorganisms in Medical, Clinical, Food and Environmental Fields, 2nd Edition

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: 30 April 2026 | Viewed by 1659

Special Issue Editors

Dr. Marcelo Andrés Umsza-Guez

E-Mail Website
Guest Editor
Food Science Postgraduate Program, Faculty of Pharmacy, Federal University of Bahia, Salvador 40170-100, BA, Brazil
Interests: food microbiology; microbial biotechnology; biotechnological use of agroindustrial waste/products; insects as a food protein source
Special Issues, Collections and Topics in MDPI journals
Dr. Karina Teixeira Magalhães-Guedes

E-Mail Website
Guest Editor
Department of Bromatological Analysis, Pharmacy Faculty, Federal University of Bahia, Salvador, Brazil
Interests: food; clinical; medical and environmental microbiology; microbial biotechnology; probiotic and psychobiotic microorganisms; biotechnology of new products/supplements for human health
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Microorganisms encompass a wide range of bacteria, microalgae, archaea, protists, fungi, and viruses. Advances in microbial biotechnology have enabled the application of microorganisms in almost all areas.

Microbial biotechnology is contributing to advances such as new microorganisms, functional foods/beverages and supplements with probiotic and psychobiotic potential; microorganisms with antimicrobial activities against pathogens; enhanced microbial agents for the biocontrol of plant and animal pests; new fermentation microorganisms; new microorganisms for bioremediation; and superpotent microbial strains modified via genome studies. In addition to the applications of microbial strains and their metabolites in medicine, the versatility of microorganisms has enhanced their study and application in biotechnology, such as in the food industry and environment field.

Therefore, this Special Issue will showcase research on the biodiversity, properties, and benefits of microorganisms in the medical, clinical, food, and environmental fields.

Topics of interest include, but are not limited to, the following:

  1. The development of new food and medical products/supplements based on microorganisms and their metabolites;
  2. New microbial strains of different origins, including those that are genetically manipulated;
  3. Microbial biodiversity for potential biotechnological applications (food, environmental, clinical, and medicine);
  4. Microbial biotechnology for the control of microbial pathogens.

Dr. Marcelo Andrés Umsza-Guez
Dr. Karina Teixeira Magalhães-Guedes
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbial biotechnology
  • microorganism diversity
  • biotechnological applications
  • functional foods
  • pathogen control

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Further information on MDPI's Special Issue policies can be found here.

Related Special Issue

Published Papers (2 papers)

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Research

17 pages, 11475 KB  
Article
Lipid Extraction and Cell Disruption Methods for Improving Biodiesel Production by Scenedesmus sp.
by Mᵃ Pilar Patón Raya, Mᵃ Lourdes Martínez Cartas and Sebastián Sánchez
Microorganisms 2026, 14(4), 731; https://doi.org/10.3390/microorganisms14040731 - 24 Mar 2026
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Abstract
Lipid recovery efficiency from microalgal biomass is a critical factor in the commercial viability of biodiesel. Scenedesmus sp. presents a robust cell wall that necessitates the evaluation of specialised disruption techniques to enhance intracellular lipid release and subsequent fuel quality. This study [...] Read more.
Lipid recovery efficiency from microalgal biomass is a critical factor in the commercial viability of biodiesel. Scenedesmus sp. presents a robust cell wall that necessitates the evaluation of specialised disruption techniques to enhance intracellular lipid release and subsequent fuel quality. This study investigated the efficacy of five cell disruption methods—microwaves, ultrasonication, lyophilisation, autoclaving, and electroporation—integrated with three distinct extraction procedures: cold extraction, Soxhlet extraction system, and microwave-assisted extraction. The qualitative and quantitative impacts of these treatments were assessed by analysing the fatty acid methyl ester (FAME) profiles via gas chromatography (GC) following transesterification. The highest total lipid yield (88.97%) was achieved through a combination of microwave disruption and Soxhlet extraction. However, the maximal proportion of methyl esters was obtained when ultrasonication was paired with microwave-assisted extraction (97.64%). Surface analysis using scanning electron microscopy (SEM) of samples subjected to different disruption procedures could support the conclusions. Similarly, when the microalgal biomass was lyophilised beforehand, microwave extraction increased the oleic acid content. These results indicate that the choice of disruption and extraction protocols significantly influences both lipid recovery rate and the proportion of fatty acids in the chemical composition of microalgae. Tailoring these processes is essential for optimising the fatty acid profile for high-quality biodiesel production. Full article
(This article belongs to the Special Issue Microbial Biotechnology: The Biodiversity, Properties and Benefits of Microorganisms in Medical, Clinical, Food and Environmental Fields, 2nd Edition)
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13 pages, 5248 KB  
Article
An Agrobacterium tumefaciens EHA105-Based GAANTRY Recipient Strain Generates High-Quality Transgenic Arabidopsis and Potato
by Upul Hathwaik, Leyla Hathwaik, Redeat Tibebu, Nic Nottingham, Teruko Oosumi, Troy Weeks, Roger Thilmony and James G. Thomson
Microorganisms 2026, 14(2), 421; https://doi.org/10.3390/microorganisms14020421 - 11 Feb 2026
Viewed by 1004
Abstract
The GAANTRY (Gene Assembly in Agrobacterium by Nucleic acid Transfer using Recombinase technology) system enables efficient gene stacking within an Agrobacterium T-DNA. Using unidirectional site-specific recombinases and alternating selection markers, it allows precise, sequential assembly of multiple genes directly within an [...] Read more.
The GAANTRY (Gene Assembly in Agrobacterium by Nucleic acid Transfer using Recombinase technology) system enables efficient gene stacking within an Agrobacterium T-DNA. Using unidirectional site-specific recombinases and alternating selection markers, it allows precise, sequential assembly of multiple genes directly within an Agrobacterium virulence plasmid. Here, we modified Agrobacterium tumefaciens strain EHA105 to create JGT105 as a GAANTRY recipient and constructed a 15.8 kb T-DNA containing five cargo sequences. We compared the performance of the JGT105 5-stack strain against a conventional binary vector carrying the same cargo sequences in Arabidopsis and potato transformation. The transformation efficiencies were comparable for the GAANTRY strain and the binary vector (potato: 83% vs. 82%; Arabidopsis: 1.73% vs. 1.95%). Single T-DNA insertion frequencies were also similar between the two systems (17.6% for GAANTRY vs. 24.5% for the binary vector construct in potato; 10.3% vs. 18.2% in Arabidopsis, respectively). Notably, the GAANTRY construct had significantly reduced vector backbone transfer in potato (10.0% vs. 26.5%) for the binary vector, whereas rates were higher in Arabidopsis (37.5% vs. 48.9%). These results show that the JGT105 GAANTRY strain is an effective T-DNA delivery system, matching binary vector transformation efficiency while offering lower backbone integration frequency, facilitating the generation of high-quality, multi-gene transgenic plants. Full article
(This article belongs to the Special Issue Microbial Biotechnology: The Biodiversity, Properties and Benefits of Microorganisms in Medical, Clinical, Food and Environmental Fields, 2nd Edition)
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