Antimicrobial Resistance and Molecular Tracing of Foodborne Pathogens 2.0

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: closed (30 April 2023) | Viewed by 2129

Special Issue Editors


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Guest Editor
Department of Microbiology and Parasitology, Universidad de Santiago de Compostela, Santiago de Compostela, Spain
Interests: beta lactamase; Enterobacteriaceae; E.coli; antibiotic resistances

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Guest Editor
Division of Epidemiology, Zoonoses and Antimicrobial Resistance, Department Biological Safety, Federal Institute for Risk Assessment (BfR), Diedersdorfer Weg 1, D-12277 Berlin, Germany
Interests: bacteriophages (phages); plasmids; horizontal gene transfer; antimicrobial resistance; genomics; zoonoses; ESKAPE-bacteria
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Special Issue Information

Dear Colleagues,

This Special Issue is the continuation of our 2019 Special Issue "Antimicrobial Resistance and Molecular Tracing of Foodborne Pathogens".

https://www.mdpi.com/journal/microorganisms/special_issues/AMR_molecular_tracing_foodborne

Foodborne-associated pathogens pose a serious health risk in developing countries and remain widespread throughout high-income regions worldwide. In the European Union (EU), more than 200,000 human cases are reported every year, but the real incidence is thought to be significantly higher. To improve food safety, detailed information on pathogens, their most important sources, as well as critical steps of the food production chain that are mainly involved in contamination of food products are needed. In contrast to culture-dependent bacterial detection procedures, molecular tracing methods are on the rise as they provide more detailed information which is relevant for the assessment of target bacteria. The use of next-generation sequencing (NGS) strategies for genomic and/or metagenomic analysis allows the highest resolution of the genetic configuration of target bacteria and/or information on the composition of the bacterial load of a sample, respectively. In this context, comprehensive genetic information (i.e., specific clonal lineages, antimicrobial/biocide resistance and virulence genes, mobile genetic elements) was collected.

In addition to information on the presence and absence of specific foodborne bacteria, many countries have implemented phenotypic antimicrobial resistance (AMR) testing in their annual monitoring programs to determine trends in AMR development. However, phenotypic resistance data often do not provide enough information to understand the dynamics of the diversity and vertical/horizontal dissemination of resistance determinants. As individual resistance determinants represent different impacts for human and animal health, detailed information on the genetic basis of a resistance, as well as the localization of the determinant within the bacterial genome is desired.

This Research Topic will deal with ongoing information on the use of molecular tracing data and/or the description of antimicrobial resistances in foodborne pathogens. As mobile genetic elements are important drivers for resistance dissemination, reports on the occurrence, modular composition, diversity, and properties of AMR-carrying plasmids are welcome. We encourage the submission of research articles, genome descriptions (foodborne bacteria and their plasmids, temperate phages and transposons/integrons), and reviews that examine and/or assess the biology and ecology of foodborne pathogens and/or AMR plasmids. We are also interested in novel technical approaches for molecular tracing of bacteria and mobile genetic elements data.

Manuscripts within this Research Topic should provide insight into at least one of the following subjects:

  • Use of molecular methods for foodborne pathogen tracing;
  • Occurrence and distribution of foodborne bacteria in the food chain (i.e., livestock, food products);
  • Prevalence and surveillance studies of bacteria or bacteria carrying important resistances against antibiotics/biocides;
  • Dissemination and transmission of plasmid types that are associated with important antimicrobial resistances;
  • Novel bacterial clones (clonal lineages) that are associated with antimicrobial resistances or that are hosts of prevalent plasmid prototypes.

Dr. Isidro García-Meniño
Dr. Jens André Hammerl
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • foodborne pathogens
  • food safety
  • occurrence
  • distribution
  • detection
  • transmission
  • antimicrobial resistance
  • next-generation sequencing

Published Papers (1 paper)

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Research

14 pages, 1335 KiB  
Article
Differences in the Propensity of Different Antimicrobial Resistance Determinants to Be Disseminated via Transformation in Campylobacter jejuni and Campylobacter coli
by Zahra Hanafy, Jason A. Osborne, William G. Miller, Craig T. Parker, Jonathan W. Olson, James H. Jackson III and Sophia Kathariou
Microorganisms 2022, 10(6), 1194; https://doi.org/10.3390/microorganisms10061194 - 10 Jun 2022
Cited by 2 | Viewed by 1783
Abstract
Campylobacter jejuni and Campylobacter coli are leading zoonotic foodborne pathogens, and the drugs of choice for human campylobacteriosis are macrolides (e.g., erythromycin) and fluoroquinolones. C. jejuni and C. coli are naturally competent for transformation via naked DNA uptake, but potential differences in transformation [...] Read more.
Campylobacter jejuni and Campylobacter coli are leading zoonotic foodborne pathogens, and the drugs of choice for human campylobacteriosis are macrolides (e.g., erythromycin) and fluoroquinolones. C. jejuni and C. coli are naturally competent for transformation via naked DNA uptake, but potential differences in transformation frequency (TF) for different antimicrobial resistance (AMR) markers remain poorly understood. We determined TFs for resistance to different antibiotics using as recipient a derivative of C. jejuni NCTC 11168 (strain SN:CM) with donor DNA from multidrug-resistant C. jejuni or C. coli. TF for nalidixic acid resistance ranked significantly highest (~1.4 × 10−3), followed by resistance to streptomycin and gentamicin. Tetracycline resistance via chromosomal tet(O) was less commonly transferred (~7.6 × 10−7), while transformation to erythromycin resistance was rare (≤4.7 × 10−8). We also determined TFs with the contemporary poultry-derived strains C. jejuni FSIS 11810577 and C. coli FSIS 1710488 as recipients. TFs to nalidixic acid and streptomycin resistance remained the highest (~7 × 10−4). However, TF for gentamicin resistance was remarkably low in certain recipient–donor combinations, while average TF for erythromycin resistance was noticeably higher (~3 × 10−6) than with SN:CM. Findings from this experimental model provide insights into factors that may impact transformation-mediated transfer of AMR leading to AMR dissemination in the agricultural ecosystem. Full article
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