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Microorganisms
Special Issues
The Pathogenic Epidemiology of Important Swine Diseases
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The Pathogenic Epidemiology of Important Swine Diseases

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Veterinary Microbiology".

Deadline for manuscript submissions: closed (29 February 2024) | Viewed by 4082

Special Issue Editor

Dr. Shujie Wang

E-Mail Website
Guest Editor
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
Interests: streptococcus suis

Special Issue Information

Dear Colleagues,

Clinically, important swine diseases are caused by several important porcine bacteria and viruses, including porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus, porcine circovirus, African swine fever virus, porcine epidemic diarrhea virus, Streptococcus suis, Haemophilus parasuisis, etc. As a result of globalization, the uncontrolled movements of animals can easily lead to the cross-border spread of epizootic diseases. Intensive concentrations of swine raised may change the ecology of many swine diseases and encourage the emergence of new diseases. Therefore, continuous epidemiological surveillance and research on the pathogenicity of new circulating bacterial or viral strains are essential for disease prevention and control.

The aim of this Special Issue is to present a collection of articles that provide an adequate multidisciplinary platform for research into important swine diseases. Manuscripts covering pathogenic epidemiology and mechanisms of research relating to swine bacterial and viral diseases, including those that address fundamental questions relating to the resistance of bacteria and the pathogenesis of virus and bacteria.

As a Guest Editor of the Special Issue, I invite you to submit research articles, review articles, and short communications related to viral and bacterial infection, epidemiology, pathogenesis, immunology, and the resistance of bacteria.

Dr. Shujie Wang
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
  • pseudorabies virus
  • porcine circovirus
  • African swine fever virus
  • porcine epidemic diarrhea virus
  • Streptococcus suis
  • Haemophilus parasuis
  • epidemiology
  • pathogenesis
  • immunology
  • resistance

Published Papers (3 papers)

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Research

14 pages, 13925 KiB  
Article
Establishment and Application of a Quadruplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assay for Differentiation of Porcine Reproductive and Respiratory Syndrome Virus, Porcine Circovirus Type 2, Porcine Circovirus Type 3, and Streptococcus suis
by Geng Wang, Hechao Zhu, Cunlin Zhan, Pin Chen, Bin Wu, Zhong Peng, Ping Qian and Guofu Cheng
Microorganisms 2024, 12(3), 427; https://doi.org/10.3390/microorganisms12030427 - 20 Feb 2024
Viewed by 788
Abstract
Respiratory illnesses present a significant threat to porcine health, with co-infections involving Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), Streptococcus suis (SS), Porcine Circovirus Type 2 (PCV2), and Porcine Circovirus Type 3 (PCV3) acting as the primary causative agents. As a [...] Read more.
Respiratory illnesses present a significant threat to porcine health, with co-infections involving Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), Streptococcus suis (SS), Porcine Circovirus Type 2 (PCV2), and Porcine Circovirus Type 3 (PCV3) acting as the primary causative agents. As a result, the precise diagnosis of PRRSV, PCV2, PCV3 and SS is of paramount importance in the prevention and control of respiratory diseases in swine. Therefore, we conducted a molecular bioinformatical analysis to concurrently detect and differentiate PRRSV, PCV2, PCV3 and SS. We selected the ORF6 gene of PRRSV, the ORF2 gene of PCV2 and PCV3, and the glutamate dehydrogenase (GDH) gene of SS as targets. Specific primers and probes were designed for each pathogen, and following meticulous optimization of reaction conditions, we established a multiple TaqMan fluorescence quantitative PCR detection method. Subsequently, we subjected this method to a comprehensive assessment, evaluating its specificity, sensitivity, and repeatability. The research results demonstrated that the established multiple TaqMan fluorescence quantitative PCR detection method displays displayed exemplary specificity, with no instances of cross-reactivity with other pathogens. The method’s minimum detection concentrations for PRRSV, PCV2, PCV3, and SS were 2.80 × 101 copies/µL, 1.96 × 102 copies/µL, 2.30 × 102 copies/µL, and 1.75 × 103 copies/µL, respectively. When applied to the analysis of 30 clinical samples, the results closely mirrored those obtained through Chinese standard uniplex real-time qPCR detection method for PRRSV, as well as the general PCR methods for SS, PCV2, and PCV3. This study underscores the robust specificity, high sensitivity, and consistent stability of the multiple TaqMan fluorescence quantitative PCR detection method that we have developed. It is ideally suited to the clinical monitoring of PRRSV, PCV2, PCV3, and SS, and it carries significant importance in ongoing efforts to prevent and manage respiratory diseases in porcine populations. Full article
(This article belongs to the Special Issue The Pathogenic Epidemiology of Important Swine Diseases)
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18 pages, 10092 KiB  
Article
Genetic and Evolutionary Analysis of Porcine Deltacoronavirus in Guangxi Province, Southern China, from 2020 to 2023
by Biao Li, Yeheng Gao, Yan Ma, Kaichuang Shi, Yuwen Shi, Shuping Feng, Yanwen Yin, Feng Long and Wenchao Sun
Microorganisms 2024, 12(2), 416; https://doi.org/10.3390/microorganisms12020416 - 19 Feb 2024
Viewed by 648
Abstract
Porcine deltacoronavirus (PDCoV) has shown large-scale global spread since its discovery in Hong Kong in 2012. In this study, a total of 4897 diarrheal fecal samples were collected from the Guangxi province of China from 2020 to 2023 and tested using RT-qPCR. In [...] Read more.
Porcine deltacoronavirus (PDCoV) has shown large-scale global spread since its discovery in Hong Kong in 2012. In this study, a total of 4897 diarrheal fecal samples were collected from the Guangxi province of China from 2020 to 2023 and tested using RT-qPCR. In total, 362 (362/4897, 7.39%) of samples were positive for PDCoV. The S, M, and N gene sequences were obtained from 34 positive samples after amplification and sequencing. These PDCoV gene sequences, together with other PDCoV S gene reference sequences from China and other countries, were analyzed. Phylogenetic analysis revealed that the Chinese PDCoV strains have diverged in recent years. Bayesian analysis revealed that the new China 1.3 lineage began to diverge in 2012. Comparing the amino acids of the China 1.3 lineage with those of other lineages, the China 1.3 lineage showed variations of mutations, deletions, and insertions, and some variations demonstrated the same as or similar to those of the China 1.2 lineage. In addition, recombination analysis revealed interlineage recombination in CHGX-MT505459-2019 and CHGX-MT505449-2017 strains from Guangxi province. In summary, the results provide new information on the prevalence and evolution of PDCoV in Guangxi province in southern China, which will facilitate better comprehension and prevention of PDCoV. Full article
(This article belongs to the Special Issue The Pathogenic Epidemiology of Important Swine Diseases)
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11 pages, 2478 KiB  
Article
Seroepidemiology Study of Porcine Epidemic Diarrhea Virus in Mexico by Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Fragment of N-Terminus Domain Spike Protein
by Eduardo García-González, José Luis Cerriteño-Sánchez, Julieta Sandra Cuevas-Romero, José Bryan García-Cambrón, Francisco Jesus Castañeda-Montes and Francisco Villaseñor-Ortega
Microorganisms 2023, 11(7), 1843; https://doi.org/10.3390/microorganisms11071843 - 20 Jul 2023
Cited by 1 | Viewed by 1143
Abstract
Porcine epidemic diarrhea (PED) is an intestinal disease caused by the porcine epidemic diarrhea virus (PEDV) and affects Mexico’s swine industry. Despite the disease initially being described in Mexico in 2013, there has been no research into the virus’s seroepidemiology carried out in [...] Read more.
Porcine epidemic diarrhea (PED) is an intestinal disease caused by the porcine epidemic diarrhea virus (PEDV) and affects Mexico’s swine industry. Despite the disease initially being described in Mexico in 2013, there has been no research into the virus’s seroepidemiology carried out in Mexico. Thus, the goal of this study was to develop an indirect ELISA (iELISA) based on a recombinant N-terminal domain truncated spike (S) protein (rNTD-S) of PEDV to evaluate serum obtained from different pig-producing states in Mexico. A total of 1054 sera were collected from pig farms, slaughterhouses, and backyard production in the states of Aguascalientes, Guanajuato, Hidalgo, Jalisco, Morelos, Queretaro, Sinaloa, and Veracruz between 2019 and 2021. The rNTD-S protein was expressed in E. coli BL21 (DE3) cells. Negative and positive serum samples used in the iELISA were previously tested by Western blot. According to our findings, 61.66% of the serum samples (650/1054) were positive, with Jalisco having the highest percentage of positive samples, at a rate of 21.44% (226/1054). This is the first seroepidemiology study of PEDV carried out in Mexico, revealing that the virus is still circulating since the initial outbreak; furthermore, it provides an overview of PEDV’s spread and high level of persistence across the country’s key swine-producing states. Full article
(This article belongs to the Special Issue The Pathogenic Epidemiology of Important Swine Diseases)
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