Steroid Profiling in Health and Disease

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Lipid Metabolism".

Deadline for manuscript submissions: closed (31 July 2020) | Viewed by 8100

Special Issue Editors


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Guest Editor
Centre for Cardiovascular Science, University of Edinburgh, Queen’s Medical Research Institute, 47, Little France Crescent, Edinburgh EH16 4TJ, UK
Interests: glucocorticoids; steroids; mass spectrometry; metabolism

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Guest Editor
The University of Edinburgh Centre for Integrative Physiology and Centre for Cardiovascular Science Edinburgh EH8 9XD, UK
Interests: glucocorticoids; metabolic disease; steroid metabolism

Special Issue Information

Dear Colleagues,

In the last decade, endocrinologists have moved away from single steroid assays by immunoassay to more informative profiles, requiring step-changes in analytical approaches integrated with bioinformatic workflows. This edition showcases current technologies, such as steroid and metabolite profiling, metabolomics, machine learning and pathway analysis, to highlight the value of these holistic approaches. Papers describing application in health, disease diagnostics, biomarkers, nutrition, pharmacology and toxicology to illustrate potential for advancing personalised medicine are particularly encouraged. Primary papers, technical notes and reviews related to steroid, sterol and associated molecules (e.g., Vitamin D, phytosteroids) are all welcome.

Prof. Ruth Andrew
Dr. Dawn Livingstone
Guest Editors

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Keywords

  • metabolite profiling and metabolomics
  • steroids
  • sterols
  • biomarkers

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Published Papers (1 paper)

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Review

44 pages, 2913 KiB  
Review
Vitamin D Metabolism and Profiling in Veterinary Species
by Emma A. Hurst, Natalie Z. Homer and Richard J. Mellanby
Metabolites 2020, 10(9), 371; https://doi.org/10.3390/metabo10090371 - 15 Sep 2020
Cited by 26 | Viewed by 7778
Abstract
The demand for vitamin D analysis in veterinary species is increasing with the growing knowledge of the extra-skeletal role vitamin D plays in health and disease. The circulating 25-hydroxyvitamin-D (25(OH)D) metabolite is used to assess vitamin D status, and the benefits of analysing [...] Read more.
The demand for vitamin D analysis in veterinary species is increasing with the growing knowledge of the extra-skeletal role vitamin D plays in health and disease. The circulating 25-hydroxyvitamin-D (25(OH)D) metabolite is used to assess vitamin D status, and the benefits of analysing other metabolites in the complex vitamin D pathway are being discovered in humans. Profiling of the vitamin D pathway by liquid chromatography tandem mass spectrometry (LC-MS/MS) facilitates simultaneous analysis of multiple metabolites in a single sample and over wide dynamic ranges, and this method is now considered the gold-standard for quantifying vitamin D metabolites. However, very few studies report using LC-MS/MS for the analysis of vitamin D metabolites in veterinary species. Given the complexity of the vitamin D pathway and the similarities in the roles of vitamin D in health and disease between humans and companion animals, there is a clear need to establish a comprehensive, reliable method for veterinary analysis that is comparable to that used in human clinical practice. In this review, we highlight the differences in vitamin D metabolism between veterinary species and the benefits of measuring vitamin D metabolites beyond 25(OH)D. Finally, we discuss the analytical challenges in profiling vitamin D in veterinary species with a focus on LC-MS/MS methods. Full article
(This article belongs to the Special Issue Steroid Profiling in Health and Disease)
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