Metabolomic Profiles in Nutrition and Metabolic Health

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Nutrition and Metabolism".

Deadline for manuscript submissions: closed (25 May 2020) | Viewed by 9986

Special Issue Editor

University of Utah and Cancer Control and Population Sciences Program, Department of Nutrition and Integrative Physiology, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
Interests: diet; nutrition; metabolic health; epidemiology; biomarkers; cancer; diabetes
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Diet, as a modifiable behavior, is of key interest among the public for promoting health and preventing and managing disease. However, research in this area has long been beset by difficulties in dietary measurement owing to its complexity and the limitations of common dietary assessment methods, including self-reported questionnaires. The resulting measurement error and biases can lead to conflicting literature and a lack of consensus on some diet-related hypotheses. Metabolomics affords the opportunity to strengthen dietary measurement through the development of objective dietary biomarkers, including food intake biomarkers, biomarkers of endogenous effect, and nutritional status. Moreover, because metabolomics captures endogenous response to food intake that is downstream of genetic and other environmental influences, it presents a major opportunity to unravel the influence of diet on metabolic health and the molecular underpinnings of diet–disease relationships. This Special Issue highlights the use of metabolomics in nutrition and metabolism research. Specific areas include, but are not limited to, dietary biomarker development, utilizing metabolomics to explore diet-related metabolic health, and/or the biological mechanisms underlying dietary relationships with disease.

Dr. Mary C. Playdon
Guest Editor

Manuscript Submission Information

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Keywords

  • Diet;
  • Nutrition;
  • Metabolism;
  • Biomarkers;
  • Epidemiology;
  • Clinical research;
  • Interventions;
  • Dietary assessment.

Published Papers (2 papers)

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Research

11 pages, 556 KiB  
Article
Feasibility of Mass-Spectrometry to Lower Cost and Blood Volume Requirements for Assessment of B Vitamins in Patients Undergoing Bariatric Surgery
by Seth Armah, Mario G. Ferruzzi and Nana Gletsu-Miller
Metabolites 2020, 10(6), 240; https://doi.org/10.3390/metabo10060240 - 10 Jun 2020
Cited by 7 | Viewed by 2392
Abstract
Bariatric surgery induces deficiencies in a combination of B vitamins. However, high costs and a large blood volume requirement are barriers to routine screening. We adapted and validated a method coupling tandem mass spectrometry (MS/MS) with high-performance liquid chromatography (HPLC) to facilitate cost-effective [...] Read more.
Bariatric surgery induces deficiencies in a combination of B vitamins. However, high costs and a large blood volume requirement are barriers to routine screening. We adapted and validated a method coupling tandem mass spectrometry (MS/MS) with high-performance liquid chromatography (HPLC) to facilitate cost-effective analysis for simultaneous detection of B vitamins in low volumes of plasma. Based on existing methods, pooled plasma was extracted using hexane and acetonitrile and seven B vitamin analytes were separated using HPLC. Detection was performed with an Agilent 6460 triple quadrupole tandem mass spectrometer (MS/MS) using electrospray ionization in the positive ion mode. We evaluated linearity, recovery, precision, and limit of detection, as well as costs of the assay. We evaluated seven B vitamins from plasma; five (riboflavin, nicotinamide, pantothenic acid, pyridoxine, and biotin) were detected and quantified with precision and linearity. Recovery ranged from 63 to 81% for each of the vitamins, except for nicotinamide—the recovery of which was suppressed to 40%, due to plasma matrix effects. We demonstrated the feasibility of the HPLC–MS/MS method for use in patients who undergo bariatric surgery by analyzing pooled plasma from patients with a lower cost and blood volume than had we sent the samples to a commercial laboratory. It is advantageous and feasible, in terms of low cost and blood volume requirement, to simultaneously measure plasma concentrations of B vitamins using HPLC–MS/MS. With further improvements, the method may enable personalized nutritional assessment for the nutritionally compromised, bariatric surgery population. Full article
(This article belongs to the Special Issue Metabolomic Profiles in Nutrition and Metabolic Health)
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24 pages, 2372 KiB  
Article
Metabolic Profiling of Human Plasma and Urine, Targeting Tryptophan, Tyrosine and Branched Chain Amino Acid Pathways
by Andrea Anesi, Josep Rubert, Kolade Oluwagbemigun, Ximena Orozco-Ruiz, Ute Nöthlings, Monique M.B. Breteler and Fulvio Mattivi
Metabolites 2019, 9(11), 261; https://doi.org/10.3390/metabo9110261 - 01 Nov 2019
Cited by 48 | Viewed by 7186
Abstract
Tryptophan and tyrosine metabolism has a major effect on human health, and disorders have been associated with the development of several pathologies. Recently, gut microbial metabolism was found to be important for maintaining correct physiology. Here, we describe the development and validation of [...] Read more.
Tryptophan and tyrosine metabolism has a major effect on human health, and disorders have been associated with the development of several pathologies. Recently, gut microbial metabolism was found to be important for maintaining correct physiology. Here, we describe the development and validation of a UHPLC-ESI-MS/MS method for targeted quantification of 39 metabolites related to tryptophan and tyrosine metabolism, branched chain amino acids and gut-derived metabolites in human plasma and urine. Extraction from plasma was optimised using 96-well plates, shown to be effective in removing phospholipids. Urine was filtered and diluted ten-fold. Metabolites were separated with reverse phase chromatography and detected using triple quadrupole MS. Linear ranges (from ppb to ppm) and correlation coefficients (r2 > 0.990) were established for both matrices independently and the method was shown to be linear for all tested metabolites. At medium spiked concentration, recovery was over 80% in both matrices, while analytical precision was excellent (CV < 15%). Matrix effects were minimal and retention time stability was excellent. The applicability of the methods was tested on biological samples, and metabolite concentrations were found to be in agreement with available data. The method allows the analysis of up to 96 samples per day and was demonstrated to be stable for up to three weeks from acquisition. Full article
(This article belongs to the Special Issue Metabolomic Profiles in Nutrition and Metabolic Health)
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