Imaging Mass Spectrometry in Metabolome

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Advances in Metabolomics".

Deadline for manuscript submissions: closed (31 January 2021) | Viewed by 11716

Special Issue Editors

Department of Chemistry, University at Buffalo, Buffalo, NY 14260-3000, USA
Interests: mass spectrometry; low-flow electrospray ionization; biomarker identification; metabolomics; proteomics; molecular imaging mass spectrometry; tandem mass spectrometry
Department of Environmental Sciences, Baylor University, Waco, TX, USA
Interests: analytical chemistry
Louisiana State University, Baton Rouge, LA, USA
Interests: physical chemistry; analytical chemistry; mass spectrometry; laser ablation

Special Issue Information

Dear Colleagues,

The ability to delineate the spatial distributions of molecules at a surface in an untargeted fashion has led to a dramatic growth in imaging mass spectrometry (IMS) (or mass spectrometry imaging, MSI) to characterize biological specimens. Hence, IMS has become a growing tool in metabolomics investigations. This Special Issue “Imaging Mass Spectrometry in Metabolomics” is focused on IMS approaches to the study of metabolic changes in biological systems. This includes the types of ionization methods and data processing approaches employed to analyze large data sets involved in metabolomics studies. Biological specimens investigated with IMS include organs (or for small animals, entire whole animal sections), plant tissues, and cell cultures. The profiling of different classes of chemical compounds (e.g., lipids, peptides, proteins, and glycans) could be involved, depending on the type of biological specimen. In addition, many studies involving IMS are focused on gleaning a deeper fundamental understanding of disease (e.g., cancer, neurodegenerative diseases, renal failure) etiology and development at the molecular level. Manuscripts which address any aspect of biological metabolic changes using IMS are desirable for this Special Issue.

Dr. Kermit K. Murray
Prof. Dr. Touradj Solouki
Prof. Troy D. Wood
Guest Editors

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • metabolomics
  • mass spectrometry imaging
  • MALDI
  • ambient ionization
  • secondary ion mass spectrometry
  • tissue

Published Papers (3 papers)

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Research

15 pages, 14141 KiB  
Article
Streamlined Multimodal DESI and MALDI Mass Spectrometry Imaging on a Singular Dual-Source FT-ICR Mass Spectrometer
by Kevin J. Zemaitis, Alexandra M. Izydorczak, Alexis C. Thompson and Troy D. Wood
Metabolites 2021, 11(4), 253; https://doi.org/10.3390/metabo11040253 - 20 Apr 2021
Cited by 15 | Viewed by 3930
Abstract
The study of biological specimens by mass spectrometry imaging (MSI) has had a profound influence in the various forms of spatial-omics over the past two decades including applications for the identification of clinical biomarker analysis; the metabolic fingerprinting of disease states; treatment with [...] Read more.
The study of biological specimens by mass spectrometry imaging (MSI) has had a profound influence in the various forms of spatial-omics over the past two decades including applications for the identification of clinical biomarker analysis; the metabolic fingerprinting of disease states; treatment with therapeutics; and the profiling of lipids, peptides and proteins. No singular approach is able to globally map all biomolecular classes simultaneously. This led to the development of many complementary multimodal imaging approaches to solve analytical problems: fusing multiple ionization techniques, imaging microscopy or spectroscopy, or local extractions into robust multimodal imaging methods. However, each fusion typically requires the melding of analytical information from multiple commercial platforms, and the tandem utilization of multiple commercial or third-party software platforms—even in some cases requiring computer coding. Herein, we report the use of matrix-assisted laser desorption/ionization (MALDI) in tandem with desorption electrospray ionization (DESI) imaging in the positive ion mode on a singular commercial orthogonal dual-source Fourier transform ion cyclotron resonance (FT-ICR) instrument for the complementary detection of multiple analyte classes by MSI from tissue. The DESI source was 3D printed and the commercial Bruker Daltonics software suite was used to generate mass spectrometry images in tandem with the commercial MALDI source. This approach allows for the generation of multiple modes of mass spectrometry images without the need for third-party software and a customizable platform for ambient ionization imaging. Highlighted is the streamlined workflow needed to obtain phospholipid profiles, as well as increased depth of coverage of both annotated phospholipid, cardiolipin, and ganglioside species from rat brain with both high spatial and mass resolution. Full article
(This article belongs to the Special Issue Imaging Mass Spectrometry in Metabolome)
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15 pages, 3680 KiB  
Article
Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification
by Michael J. Taylor, Sara Mattson, Andrey Liyu, Sylwia A. Stopka, Yehia M. Ibrahim, Akos Vertes and Christopher R. Anderton
Metabolites 2021, 11(4), 200; https://doi.org/10.3390/metabo11040200 - 27 Mar 2021
Cited by 24 | Viewed by 3765
Abstract
Single cell analysis is a field of increasing interest as new tools are continually being developed to understand intercellular differences within large cell populations. Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) is an emerging technique for single cell metabolomics. Over the years, it has [...] Read more.
Single cell analysis is a field of increasing interest as new tools are continually being developed to understand intercellular differences within large cell populations. Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) is an emerging technique for single cell metabolomics. Over the years, it has been validated that this ionization technique is advantageous for probing the molecular content of individual cells in situ. Here, we report the integration of a microscope into the optical train of the LAESI source to allow for visually informed ambient in situ single cell analysis. Additionally, we have coupled this ‘LAESI microscope’ to a drift-tube ion mobility mass spectrometer to enable separation of isobaric species and allow for the determination of ion collision cross sections in conjunction with accurate mass measurements. This combined information helps provide higher confidence for structural assignment of molecules ablated from single cells. Here, we show that this system enables the analysis of the metabolite content of Allium cepa epidermal cells with high confidence structural identification together with their spatial locations within a tissue. Full article
(This article belongs to the Special Issue Imaging Mass Spectrometry in Metabolome)
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20 pages, 5731 KiB  
Article
Analyzing Mass Spectrometry Imaging Data of 13C-Labeled Phospholipids in Camelina sativa and Thlaspi arvense (Pennycress) Embryos
by Trevor B. Romsdahl, Shrikaar Kambhampati, Somnath Koley, Umesh P. Yadav, Ana Paula Alonso, Doug K. Allen and Kent D. Chapman
Metabolites 2021, 11(3), 148; https://doi.org/10.3390/metabo11030148 - 04 Mar 2021
Cited by 12 | Viewed by 3143
Abstract
The combination of 13C-isotopic labeling and mass spectrometry imaging (MSI) offers an approach to analyze metabolic flux in situ. However, combining isotopic labeling and MSI presents technical challenges ranging from sample preparation, label incorporation, data collection, and analysis. Isotopic labeling and MSI [...] Read more.
The combination of 13C-isotopic labeling and mass spectrometry imaging (MSI) offers an approach to analyze metabolic flux in situ. However, combining isotopic labeling and MSI presents technical challenges ranging from sample preparation, label incorporation, data collection, and analysis. Isotopic labeling and MSI individually create large, complex data sets, and this is compounded when both methods are combined. Therefore, analyzing isotopically labeled MSI data requires streamlined procedures to support biologically meaningful interpretations. Using currently available software and techniques, here we describe a workflow to analyze 13C-labeled isotopologues of the membrane lipid and storage oil lipid intermediate―phosphatidylcholine (PC). Our results with embryos of the oilseed crops, Camelina sativa and Thlaspi arvense (pennycress), demonstrated greater 13C-isotopic labeling in the cotyledons of developing embryos compared with the embryonic axis. Greater isotopic enrichment in PC molecular species with more saturated and longer chain fatty acids suggest different flux patterns related to fatty acid desaturation and elongation pathways. The ability to evaluate MSI data of isotopically labeled plant embryos will facilitate the potential to investigate spatial aspects of metabolic flux in situ. Full article
(This article belongs to the Special Issue Imaging Mass Spectrometry in Metabolome)
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