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Fluorescence Spectroscopy for Materials Characterization

A special issue of Materials (ISSN 1996-1944). This special issue belongs to the section "Advanced Materials Characterization".

Deadline for manuscript submissions: 20 September 2026 | Viewed by 748

Special Issue Editors


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Guest Editor
School of Biomedical Engineering, Faculty of Engineering, Jan Koum Center for Nanoscience and Nanotechnology, Tel-Aviv University, Ramat Aviv 6997801, Israel
Interests: fluorescence lifetime and anisotropy decay; fluorescence lifetime imaging; biological imaging based on fluorescence parameters; light–tissue interaction
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Guest Editor Assistant
School of Biomedical Engineering, Faculty of Engineering, Jan Koum Center for Nanoscience and Nanotechnology, Tel-Aviv University, Ramat Aviv 6997801, Israel
Interests: fluorescence anisotropy decay; fluorescence lifetime imaging; bio-photonics; time domain and frequency domain FLIM; acousto-optics; acousto-optical crystals

Special Issue Information

Dear Colleagues,

Materials welcomes submissions to a Special Issue on Fluorescence Spectroscopy for Materials Characterization. The objective of this Special Issue is to spotlight all fields of single-molecule fluorescence optics, fluorescence molecule spectroscopy and super resolution fluorescence imaging, ranging from fundamental physics and technical and methodological questions, to their applications in chemical, biological and biomedical research, as well as in medical diagnostics.

In our Special Issue, we wish to provide authors a state-of-the-art interdisciplinary forum for the exchange of information on new technological developments, advanced applications, and fundamental questions in the field. We are soliciting invited and contributed papers concerning, but not limited to, the following areas: time-resolved methods of fluorescence spectroscopy and imaging which allow for investigations of not only the structural properties, but also the functions of molecular processes, down to the single molecule level. Therefore, we encourage submissions of works related also to fluorescence lifetime imaging (FLIM), advanced single-molecule techniques such as fluorescence correlation spectroscopy (FCS), and fluorescence coincidence analysis or single-molecule burst analysis, which are also favorite subjects of this conference. Förster resonance energy transfer (FRET) analysis, in particular, frequently benefits from conjunction with these time-resolved methods, and this issue would be very useful for discussions on their applications at the molecular level. Another topic of particular interest is the employment of the single-molecule nature of fluorescence excitation and emission to achieve sub-diffraction super-resolution in fluorescence microscopy. This has resulted in new microscopy modalities, such as stimulated emission depletion (STED) microscopy, single molecule localization microscopy (PALM, STORM, (d)STORM, GSD-IM), stochastic optical fluctuation microscopy (SOFI), or structured illumination microscopy (SIM) and imaging scanning microscopy (ISM) techniques.

Contributions are welcome in the form of original scientific manuscripts, reviews or perspectives, in all the above, relevant areas, but especially:

  • Techniques and methods for single molecule (SM) detection;
  • Techniques and methods for SM spectroscopy (such as FCS, FLCS, FLIM, FRET);
  • Techniques and methods for fluorescence lifetime imaging (FLIM) with one, two, or three photon excitations;
  • Techniques for single molecule manipulation;
  • Super-resolution fluorescence imaging (STED, PALM, (d)STORM, GSD-IM, SOFI, SIM and related techniques);
  • Advanced fluorescence imaging, including (time-resolved) two- and three-photon fluorescence microscopy or (time-resolved) Raman spectroscopy;
  • Medical applications of fluorescence spectroscopy and imaging;
  • Ultrasensitive biomedical diagnostics.

All submissions will be peer reviewed. Submissions should follow the Materials author guidelines and fall within the broader Materials scope. A cover letter indicating that the submission is intended for this special issue should be included.

Prof. Dr. Dror Fixler
Guest Editor

Dr. Ulugbek Saidvaliev
Guest Editor Assistant

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Materials is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescence lifetime imaging (FLIM)
  • fluorescence microscopy
  • förster resonance energy transfer
  • FLIM analysis
  • fluorescence bioimaging
  • super-resolution fluorescence imaging
  • fluorescence coincidence analysis
  • single molecule spectroscopy

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Published Papers (1 paper)

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Research

20 pages, 4441 KB  
Article
Metal-Enhanced Fluorescence of Nanocomplexes
by Alexander N. Yakunin, Sergey V. Zarkov, Yuri A. Avetisyan, Garif G. Akchurin and Valery V. Tuchin
Materials 2026, 19(6), 1258; https://doi.org/10.3390/ma19061258 - 22 Mar 2026
Viewed by 190
Abstract
Metal-enhanced fluorescence (MEF) has found widespread application in biomedical sensing and in vivo tissue imaging systems. To enhance MEF efficiency, it is necessary to optimize the interaction between the metal nanoparticle plasmon and the fluorophore molecule. The size and shape of the nanoparticle, [...] Read more.
Metal-enhanced fluorescence (MEF) has found widespread application in biomedical sensing and in vivo tissue imaging systems. To enhance MEF efficiency, it is necessary to optimize the interaction between the metal nanoparticle plasmon and the fluorophore molecule. The size and shape of the nanoparticle, the nanoscale gap between the fluorescent molecule and the nanoparticle, and the excitation wavelength are critical parameters. In this study, we propose a model for a more complete and accurate description of the processes of molecular excitation and generation of the fluorescence spectral response, introducing a new concept of effective properties for the field enhancement factor, quantum yield, and fluorescence enhancement factor. The influence of the spectral properties of both the nanostructure plasmon and the fluorophore molecule on the optimal tuning of fluorescent complexes is studied. Particular attention is paid to the analysis of the spectral properties of plasmon resonance and calculations of the near-field intensity enhancement of the plasmonic nanostructure’s excitation field. Numerical results for optimizing the MEF of fluorescent complexes based on TagRFP and gold (silver) nanorod composites are presented. The advantages of the proposed model for the optimal design of new nanomaterials with unique fluorescent properties are discussed. Full article
(This article belongs to the Special Issue Fluorescence Spectroscopy for Materials Characterization)
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