Protein Research in Pathogenic Fungi

A special issue of Journal of Fungi (ISSN 2309-608X).

Deadline for manuscript submissions: 30 April 2025 | Viewed by 465

Special Issue Editors


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Guest Editor
Department of Comparative Biochemistry and Bioanalytics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Interests: biochemistry; protein purification; proteins; fungi
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Guest Editor
Department of Comparative Biochemistry and Bioanalytics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland
Interests: biochemistry; protein purification; proteins; fungi

Special Issue Information

Dear Colleagues,

The thorough characterization and identification of fungal proteins must be prioritized in investigations into the pathogenesis of fungal infections. The structural attributes of these molecules, their localization, their enzymatic functions, and their interaction capabilities are essential in fungal cell biology and communication between pathogenic fungi and other microorganisms inhabiting the same niche, as well as between pathogenic fungi and the host. Intracellular proteins, those presenting on the fungal cell surface, and those secreted outside the cell, including proteins transported by fungal extracellular vesicles, can be involved, through multifaceted cooperation, in a range of biological processes. Investigating the differential expression of these proteins under specific environmental conditions and the variability in their interactions is necessary in elucidating infection mechanisms and developing effective strategies for the prevention and treatment of fungal diseases.

For this Special Issue, we will accept both original research articles and review papers focused on the study of fungal proteins produced by pathogenic fungi affecting plants, animals, and humans. 

Dr. Justyna Karkowska-Kuleta
Dr. Dorota Satala
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Fungi is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • protein characterization
  • fungal proteomics
  • enzymatic activity
  • protein–protein interactions
  • host–pathogen interactions
  • environmental stress response
  • differential protein expression
  • virulence factors
  • antifungal resistance
  • signaling
  • protein structure

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Published Papers (1 paper)

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Research

46 pages, 13286 KiB  
Article
Three-Dimensional Structural Heteromorphs of Mating-Type Proteins in Hirsutella sinensis and the Natural Cordyceps sinensis Insect–Fungal Complex
by Xiu-Zhang Li, Yu-Ling Li and Jia-Shi Zhu
J. Fungi 2025, 11(4), 244; https://doi.org/10.3390/jof11040244 - 23 Mar 2025
Viewed by 240
Abstract
The MAT1-1-1 and MAT1-2-1 proteins are essential for the sexual reproduction of Ophiocordyceps sinensis. Although Hirsutella sinensis has been postulated to be the sole anamorph of O. sinensis and to undergo self-fertilization under homothallism or pseudohomothallism, little is known about the three-dimensional [...] Read more.
The MAT1-1-1 and MAT1-2-1 proteins are essential for the sexual reproduction of Ophiocordyceps sinensis. Although Hirsutella sinensis has been postulated to be the sole anamorph of O. sinensis and to undergo self-fertilization under homothallism or pseudohomothallism, little is known about the three-dimensional (3D) structures of the mating proteins in the natural Cordyceps sinensis insect–fungal complex, which is a valuable therapeutic agent in traditional Chinese medicine. However, the alternative splicing and differential occurrence and translation of the MAT1-1-1 and MAT1-2-1 genes have been revealed in H. sinensis, negating the self-fertilization hypothesis but rather suggesting the occurrence of self-sterility under heterothallic or hybrid outcrossing. In this study, the MAT1-1-1 and MAT1-2-1 proteins in 173 H. sinensis strains and wild-type C. sinensis isolates were clustered into six and five clades in the Bayesian clustering trees and belonged to 24 and 21 diverse AlphaFold-predicted 3D structural morphs, respectively. Over three-quarters of the strains/isolates contained either MAT1-1-1 or MAT1-2-1 proteins but not both. The diversity of the heteromorphic 3D structures of the mating proteins suggested functional alterations of the proteins and provided additional evidence supporting the self-sterility hypothesis under heterothallism and hybridization for H. sinensis, Genotype #1 of the 17 genome-independent O. sinensis genotypes. The heteromorphic stereostructures and mutations of the MAT1-1-1 and MAT1-2-1 proteins in the wild-type C. sinensis isolates and natural C. sinensis insect–fungi complex suggest that there are various sources of the mating proteins produced by two or more cooccurring heterospecific fungal species in natural C. sinensis that have been discovered in mycobiotic, molecular, metagenomic, and metatranscriptomic studies, which may inspire future studies on the biochemistry of mating and pheromone receptor proteins and the reproductive physiology of O. sinensis. Full article
(This article belongs to the Special Issue Protein Research in Pathogenic Fungi)
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