Journal of Functional Biomaterials

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22 pages, 5628 KiB

Open AccessArticle

Effect of Pixel Offset Adjustments for XY Plane Dimensional Compensation in Digital Light Processing 3D Printing on the Surface Trueness and Fit of Zirconia Crowns

by KeunBaDa Son, Ji-Min Lee, Kyoung-Jun Jang, Sang-Kyu Lee, Jun Ho Hwang, Jong Hoon Lee, Hyun Deok Kim, So-Yeun Kim and Kyu-Bok Lee

J. Funct. Biomater. 2025, 16(3), 103; https://doi.org/10.3390/jfb16030103 - 14 Mar 2025

Abstract

This study aimed to evaluate the effect of pixel offset adjustments in digital light processing (DLP) three-dimensional (3D) printing on the marginal and internal fit and surface trueness of zirconia crowns. Zirconia crowns were designed using dental computer-aided design software (Dentbird; Imagoworks) and [...] Read more.

This study aimed to evaluate the effect of pixel offset adjustments in digital light processing (DLP) three-dimensional (3D) printing on the marginal and internal fit and surface trueness of zirconia crowns. Zirconia crowns were designed using dental computer-aided design software (Dentbird; Imagoworks) and fabricated with a vat photopolymerization DLP 3D printer (TD6+; 3D Controls) under three pixel offset conditions (−1, 0, and 1). Pixel offset refers to the controlled modification of the outermost pixels in the XY plane during printing to compensate for potential dimensional inaccuracies. The marginal and internal fit was assessed using a triple-scan protocol and quantified using root mean square (RMS) values. Surface trueness was evaluated by measuring RMS, positive and negative errors between the designed and fabricated crowns. Statistical analyses included one-way ANOVA and Pearson correlation analysis (α = 0.05). The Pixel offset had a significant effect on fit accuracy and surface trueness (p < 0.05). Higher pixel offsets increased marginal discrepancies (p = 0.004), with the marginal gap exceeding 120 µm at a pixel offset of 1 (114.5 ± 14.6 µm), while a pixel offset of −1 (85.5 ± 18.6 µm) remained within acceptable limits (p = 0.003). Surface trueness worsened with increasing pixel offset, showing greater positive errors (p < 0.001). Optimizing pixel offset in DLP 3D printing is crucial to ensuring clinically acceptable zirconia crowns. Improper settings may increase marginal discrepancies and surface errors, compromising restoration accuracy. Full article

(This article belongs to the Special Issue Advanced 3D Printing Biomaterials)

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14 pages, 5726 KiB

Open AccessArticle

Personalized 3D-Printed Prostheses for Bone Defect Reconstruction After Tumor Resection in the Foot and Ankle

by Chang-Jin Yon, Byung-Chan Choi, Jung-Min Lee and Si-Wook Lee

J. Funct. Biomater. 2025, 16(2), 62; https://doi.org/10.3390/jfb16020062 - 11 Feb 2025

Viewed by 407

Abstract

Three-dimensional (3D)-printing technology is revolutionizing orthopedic oncology by providing precise, customized solutions for complex bone defects following tumor resection. Traditional modular endoprostheses are prone to complications such as fretting corrosion and implant failure, underscoring the need for innovative approaches. This case series reports [...] Read more.

Three-dimensional (3D)-printing technology is revolutionizing orthopedic oncology by providing precise, customized solutions for complex bone defects following tumor resection. Traditional modular endoprostheses are prone to complications such as fretting corrosion and implant failure, underscoring the need for innovative approaches. This case series reports on three patients treated with 3D-printed, patient-specific prostheses and cutting guides. Preoperative CT and MRI data were used to design implants tailored to each patient’s anatomy, manufactured using electron beam melting technology with a titanium–aluminum–vanadium alloy. Functional outcomes showed significant improvements: in Case I, AOFAS improved from 71 to 96, and VAS decreased from 6 to 1; in Case II, AOFAS increased from 65 to 79, and VAS decreased from 5 to 3. Radiographic evaluations demonstrated stable prosthesis placement and early evidence of bone integration in Cases I and II, while in Case III, localized disease control was achieved before systemic progression. This case series highlights the transformative potential of 3D-printed prostheses in addressing the challenges of reconstructing anatomically complex defects. By enabling precise tumor resection and improving functional outcomes, this approach can advance current practices in orthopedic oncology. Further research should explore larger cohorts and use cost-effectiveness analyses to validate these findings and facilitate broader clinical adoption. Full article

(This article belongs to the Special Issue Advanced 3D Printing Biomaterials)

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15 pages, 4111 KiB

Open AccessArticle

Biaxial Flexural Strength and Vickers Hardness of 3D-Printed and Milled 5Y Partially Stabilized Zirconia

by Sebastian Hetzler, Carina Hinzen, Stefan Rues, Clemens Schmitt, Peter Rammelsberg and Andreas Zenthöfer

J. Funct. Biomater. 2025, 16(1), 36; https://doi.org/10.3390/jfb16010036 - 20 Jan 2025

Viewed by 449

Abstract

This study compares the mechanical properties of 5-mol% yttria partially stabilized zirconia (5Y-PSZ) materials, designed for 3D printing or milling. Three 5Y-PSZ materials were investigated: printed zirconia (PZ) and two milled zirconia materials, VITA-YZ-XT (MZ-1) and Cercon xt (MZ-2). PZ samples were made [...] Read more.

This study compares the mechanical properties of 5-mol% yttria partially stabilized zirconia (5Y-PSZ) materials, designed for 3D printing or milling. Three 5Y-PSZ materials were investigated: printed zirconia (PZ) and two milled zirconia materials, VITA-YZ-XT (MZ-1) and Cercon xt (MZ-2). PZ samples were made from a novel ceramic suspension via digital light processing and divided into three subgroups: PZ-HN-ZD (horizontal nesting, printed with Zipro-D Dental), PZ-VN-Z (vertical nesting, printed with Zipro-D Dental) and PZ-VN-Z (vertical nesting, printed with Zipro Dental). Key outcomes included biaxial flexural strength (ISO 6872) and Vickers hardness (n ≥ 23 samples/subgroup). Microstructure and grain size were analyzed using light and scanning electron microscopy. Printed specimens exhibited biaxial flexural strengths of 1059 ± 178 MPa (PZ-HN-ZD), 797 ± 135 MPa (PZ-VN-ZD), and 793 ± 75 MPa (PZ-VN-Z). Milled samples showed strengths of 745 ± 96 MPa (MZ-1) and 928 ± 87 MPa (MZ-2). Significant differences (α = 0.05) were observed, except between vertically printed groups and MZ-1. Vickers hardness was highest for PZ-VN-Z (HV0.5 = 1590 ± 24), followed by MZ-1 (HV0.5 = 1577 ± 9) and MZ-2 (HV0.5 = 1524 ± 4), with significant differences, except between PZ and MZ-1. PZ samples had the smallest grain size (0.744 ± 0.024 µm) compared to MZ-1 (0.820 ± 0.042 µm) and MZ-2 (1.023 ± 0.081 µm). All materials met ISO 6872 standards for crowns and three-unit prostheses in posterior regions. Full article

(This article belongs to the Special Issue Advanced 3D Printing Biomaterials)

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16 pages, 5700 KiB

Open AccessArticle

3D Printing of a Porous Zn-1Mg-0.1Sr Alloy Scaffold: A Study on Mechanical Properties, Degradability, and Biosafety

by Xiangyu Cao, Xinguang Wang, Jiazheng Chen, Xiao Geng and Hua Tian

J. Funct. Biomater. 2024, 15(4), 109; https://doi.org/10.3390/jfb15040109 - 18 Apr 2024

Cited by 2 | Viewed by 2823

Abstract

In recent years, the use of zinc (Zn) alloys as degradable metal materials has attracted considerable attention in the field of biomedical bone implant materials. This study investigates the fabrication of porous scaffolds using a Zn-1Mg-0.1Sr alloy through a three-dimensional (3D) printing technique, [...] Read more.

In recent years, the use of zinc (Zn) alloys as degradable metal materials has attracted considerable attention in the field of biomedical bone implant materials. This study investigates the fabrication of porous scaffolds using a Zn-1Mg-0.1Sr alloy through a three-dimensional (3D) printing technique, selective laser melting (SLM). The results showed that the porous Zn-1Mg-0.1Sr alloy scaffold featured a microporous structure and exhibited a compressive strength (CS) of 33.71 ± 2.51 MPa, a yield strength (YS) of 27.88 ± 1.58 MPa, and an elastic modulus (E) of 2.3 ± 0.8 GPa. During the immersion experiments, the immersion solution showed a concentration of 2.14 ± 0.82 mg/L for Zn²⁺ and 0.34 ± 0.14 mg/L for Sr²⁺, with an average pH of 7.61 ± 0.09. The porous Zn-1Mg-0.1Sr alloy demonstrated a weight loss of 12.82 ± 0.55% and a corrosion degradation rate of 0.36 ± 0.01 mm/year in 14 days. The Cell Counting Kit-8 (CCK-8) assay was used to check the viability of the cells. The results showed that the 10% and 20% extracts significantly increased the activity of osteoblast precursor cells (MC3T3-E1), with a cytotoxicity grade of 0, which indicates safety and non-toxicity. In summary, the porous Zn-1Mg-0.1Sr alloy scaffold exhibits outstanding mechanical properties, an appropriate degradation rate, and favorable biosafety, making it an ideal candidate for degradable metal bone implants. Full article

(This article belongs to the Special Issue Advanced 3D Printing Biomaterials)

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15 pages, 5312 KiB

Open AccessArticle

An Anti-Oxidative Bioink for Cartilage Tissue Engineering Applications

by Xin Chen, Mengni Yang, Zheng Zhou, Jingjing Sun, Xiaolin Meng, Yuting Huang, Wenxiang Zhu, Shuai Zhu, Ning He, Xiaolong Zhu, Xiaoxiao Han and Hairong Liu

J. Funct. Biomater. 2024, 15(2), 37; https://doi.org/10.3390/jfb15020037 - 2 Feb 2024

Viewed by 2479

Abstract

Since chondrocytes are highly vulnerable to oxidative stress, an anti-oxidative bioink combined with 3D bioprinting may facilitate its applications in cartilage tissue engineering. We developed an anti-oxidative bioink with methacrylate-modified rutin (RTMA) as an additional bioactive component and glycidyl methacrylate silk fibroin as [...] Read more.

Since chondrocytes are highly vulnerable to oxidative stress, an anti-oxidative bioink combined with 3D bioprinting may facilitate its applications in cartilage tissue engineering. We developed an anti-oxidative bioink with methacrylate-modified rutin (RTMA) as an additional bioactive component and glycidyl methacrylate silk fibroin as a biomaterial component. Bioink containing 0% RTMA was used as the control sample. Compared with hydrogel samples produced with the control bioink, solidified anti-oxidative bioinks displayed a similar porous microstructure, which is suitable for cell adhesion and migration, and the transportation of nutrients and wastes. Among photo-cured samples prepared with anti-oxidative bioinks and the control bioink, the sample containing 1 mg/mL of RTMA (RTMA-1) showed good degradation, promising mechanical properties, and the best cytocompatibility, and it was selected for further investigation. Based on the results of 3D bioprinting tests, the RTMA-1 bioink exhibited good printability and high shape fidelity. The results demonstrated that RTMA-1 reduced intracellular oxidative stress in encapsulated chondrocytes under H₂O₂ stimulation, which results from upregulation of COLII and AGG and downregulation of MMP13 and MMP1. By using in vitro and in vivo tests, our data suggest that the RTMA-1 bioink significantly enhanced the regeneration and maturation of cartilage tissue compared to the control bioink, indicating that this anti-oxidative bioink can be used for 3D bioprinting and cartilage tissue engineering applications in the future. Full article

(This article belongs to the Special Issue Advanced 3D Printing Biomaterials)

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21 pages, 7146 KiB

Open AccessArticle

Evaluation of Compressive and Permeability Behaviors of Trabecular-Like Porous Structure with Mixed Porosity Based on Mechanical Topology

by Long Chao, Yangdong He, Jiasen Gu, Deqiao Xie, Youwen Yang, Lida Shen, Guofeng Wu, Lin Wang and Zongjun Tian

J. Funct. Biomater. 2023, 14(1), 28; https://doi.org/10.3390/jfb14010028 - 3 Jan 2023

Cited by 12 | Viewed by 2656

Abstract

The mechanical properties and permeability properties of artificial bone implants have high-level requirements. A method for the design of trabecular-like porous structure (TLPS) with mixed porosity is proposed based on the study of the mechanical and permeability characteristics of natural bone. With this [...] Read more.

The mechanical properties and permeability properties of artificial bone implants have high-level requirements. A method for the design of trabecular-like porous structure (TLPS) with mixed porosity is proposed based on the study of the mechanical and permeability characteristics of natural bone. With this technique, the morphology and density of internal porous structures can be adjusted, depending on the implantation requirements, to meet the mechanical and permeability requirements of natural bone. The design parameters mainly include the seed points, topology optimization coefficient, load value, irregularity, and scaling factor. Characteristic parameters primarily include porosity and pore size distribution. Statistical methods are used to analyze the relationship between design parameters and characteristic parameters for precise TLPS design and thereby provide a theoretical basis and guidance. TLPS scaffolds were prepared by selective laser melting technology. First, TLPS under different design parameters were analyzed using the finite element method and permeability simulation. The results were then verified by quasistatic compression and cell experiments. The scaling factor and topology optimization coefficient were found to largely affect the mechanical and permeability properties of the TLPS. The corresponding compressive strength reached 270–580 MPa; the elastic modulus ranged between 6.43 and 9.716 GPa, and permeability was 0.6 × 10⁻⁹–21 × 10⁻⁹; these results were better than the mechanical properties and permeability of natural bone. Thus, TLPS can effectively improve the success rate of bone implantation, which provides an effective theory and application basis for bone implantation. Full article

(This article belongs to the Special Issue Advanced 3D Printing Biomaterials)

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Review

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26 pages, 4576 KiB

Open AccessReview

Integrating Machine Learning into Additive Manufacturing of Metallic Biomaterials: A Comprehensive Review

by Shangyan Zhao, Yixuan Shi, Chengcong Huang, Xuan Li, Yuchen Lu, Yuzhi Wu, Yageng Li and Luning Wang

J. Funct. Biomater. 2025, 16(3), 77; https://doi.org/10.3390/jfb16030077 - 21 Feb 2025

Viewed by 584

Abstract

The global increase in osteomuscular diseases, particularly bone defects and fractures, has driven the growing demand for metallic implants. Additive manufacturing (AM) has emerged as a transformative technology for producing high-precision metallic biomaterials with customized properties, offering significant advantages over traditional manufacturing methods. [...] Read more.

The global increase in osteomuscular diseases, particularly bone defects and fractures, has driven the growing demand for metallic implants. Additive manufacturing (AM) has emerged as a transformative technology for producing high-precision metallic biomaterials with customized properties, offering significant advantages over traditional manufacturing methods. The integration of machine learning (ML) with AM has shown great promise in optimizing the fabrication process, enhancing material performance, and predicting long-term behavior, particularly in the development of orthopedic implants and vascular stents. This review explores the application of ML in AM of metallic biomaterials, focusing on four key areas: (1) component design, where ML guides the optimization of multi-component alloys for improved mechanical and biological properties; (2) structural design, enabling the creation of intricate porous architectures tailored to specific functional requirements; (3) process control, facilitating real-time monitoring and adjustment of manufacturing parameters; and (4) parameter optimization, which reduces costs and enhances production efficiency. This review offers a comprehensive overview of four key aspects, presenting relevant research and providing an in-depth analysis of the current state of ML-guided AM techniques for metallic biomaterials. It enables readers to gain a thorough understanding of the latest advancements in this field. Additionally, the this review addresses the challenges in predicting in vivo performance, particularly degradation behavior, and how ML models can assist in bridging the gap between in vitro tests and clinical outcomes. The integration of ML in AM holds great potential to accelerate the design and production of advanced metallic biomaterials. Full article

(This article belongs to the Special Issue Advanced 3D Printing Biomaterials)

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20 pages, 2304 KiB

Open AccessReview

Inflammation Responses to Bone Scaffolds under Mechanical Stimuli in Bone Regeneration

by Junjie Wang, Bo Yuan, Ruixue Yin and Hongbo Zhang

J. Funct. Biomater. 2023, 14(3), 169; https://doi.org/10.3390/jfb14030169 - 21 Mar 2023

Cited by 4 | Viewed by 2612

Abstract

Physical stimuli play an important role in one tissue engineering. Mechanical stimuli, such as ultrasound with cyclic loading, are widely used to promote bone osteogenesis; however, the inflammatory response under physical stimuli has not been well studied. In this paper, the signaling pathways [...] Read more.

Physical stimuli play an important role in one tissue engineering. Mechanical stimuli, such as ultrasound with cyclic loading, are widely used to promote bone osteogenesis; however, the inflammatory response under physical stimuli has not been well studied. In this paper, the signaling pathways related to inflammatory responses in bone tissue engineering are evaluated, and the application of physical stimulation to promote osteogenesis and its related mechanisms are reviewed in detail; in particular, how physical stimulation alleviates inflammatory responses during transplantation when employing a bone scaffolding strategy is discussed. It is concluded that physical stimulation (e.g., ultrasound and cyclic stress) helps to promote osteogenesis while reducing the inflammatory response. In addition, apart from 2D cell culture, more consideration should be given to the mechanical stimuli applied to 3D scaffolds and the effects of different force moduli while evaluating inflammatory responses. This will facilitate the application of physiotherapy in bone tissue engineering. Full article

(This article belongs to the Special Issue Advanced 3D Printing Biomaterials)

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