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Insects
Special Issues
Insect–Microorganism Interactions
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Insect–Microorganism Interactions

A special issue of Insects (ISSN 2075-4450). This special issue belongs to the section "Insect Physiology, Reproduction and Development".

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 3945

Special Issue Editors

Dr. Anne-Nathalie Volkoff

E-Mail Website
Guest Editor
UMR 1333 INRAE/University of Montpellier DGIMI (Diversity, Genomes, Insect-Microorganism Interactions), CEDEX 5, 34 095 Montpellier, France
Interests: host–parasitoid interactions; polydnavirus; insect omics; endogenous viruses; insect-virus mutualistic interactions
Dr. Federica Calevro

E-Mail Website
Guest Editor
UMR 203 INRAE/INSA-Lyon BF2i (Functional Biology, Insects and Interactions), University of Lyon, 69621 Villeurbanne, France
Interests: nutritional-based symbiosis; insect-bacteria interactions; functional genomics; insect and bacteria omics

Special Issue Information

Dear Colleagues,

Insects, like all other organisms, live in interaction with a wide range of microbes including viruses, bacteria, archea, fungi, or protozoa. This extreme diversity of insect microbiomes has been discovered thanks to the contribution of new high-throughput “omics” tools. Insect–microorganism interactions can be transient or permanent, and cover the entire continuum from mutualistic to pathogenic. This Special Issue will particularly focus on descriptions of novel insects–microorganisms’ associations but it is also open to studies on “classic” insect-microorganism associations aiming at unraveling the dynamics of insect–microorganisms interactions, or on the ways how microbial communities affect the insect host lifestyles and physiology (development, metabolism, immunity, behavior, host–parasite interactions or plant–insect relationships).

Dr. Anne-Nathalie Volkoff
Dr. Federica Calevro
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Insects is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Viruses
  • Bacteria
  • Archaea
  • fungi
  • Protozoa
  • mutualism
  • pathogenicity
  • insect life traits
  • insect physiology
  • dynamics

Published Papers (1 paper)

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Research

13 pages, 1321 KiB  
Article
Culturing-Enriched Metabarcoding Analysis of the Oryctes rhinoceros Gut Microbiome
by Matan Shelomi and Ming-Ju Chen
Insects 2020, 11(11), 782; https://doi.org/10.3390/insects11110782 - 11 Nov 2020
Cited by 8 | Viewed by 3222
Abstract
Wood-feeding insects should have a source of enzymes like cellulases to digest their food. These enzymes can be produced by the insect, or by microbes living in the wood and/or inside the insect gut. The coconut rhinoceros beetle, Oryctes rhinoceros, is a [...] Read more.
Wood-feeding insects should have a source of enzymes like cellulases to digest their food. These enzymes can be produced by the insect, or by microbes living in the wood and/or inside the insect gut. The coconut rhinoceros beetle, Oryctes rhinoceros, is a pest whose digestive microbes are of considerable interest. This study describes the compartments of the O. rhinoceros gut and compares their microbiomes using culturing-enriched metabarcoding. Beetle larvae were collected from a coconut grove in southern Taiwan. Gut contents from the midgut and hindgut were plated on nutrient agar and selective carboxymethylcellulose agar plates. DNA was extracted from gut and fat body samples and 16S rDNA metabarcoding performed to identify unculturable bacteria. Cellulase activity tests were performed on gut fluids and microbe isolates. The midgut and hindgut both showed cellulolytic activity. Bacillus cereus, Citrobacter koseri, and the cellulolytic fungus Candida xylanilytica were cultured from both gut sections in most larvae. Metabarcoding did not find Bacillus cereus, and found that either Citrobacter koseri or Paracoccus sp. were the dominant gut microbes in any given larva. No significant differences were found between midgut and hindgut microbiomes. Bacillus cereus and Citrobacter koseri are common animal gut microbes frequently found in Oryctes rhinoceros studies while Candida xylanilytica and the uncultured Paracoccus sp. had not been identified in this insect before. Some or all of these may well have digestive functions for the beetle, and are most likely acquired from the diet, meaning they may be transient commensalists rather than obligate mutualists. Broader collection efforts and tests with antibiotics will resolve ambiguities in the beetle–microbe interactions. Full article
(This article belongs to the Special Issue Insect–Microorganism Interactions)
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