Experimental and Field Approaches Using Pollinating Insects as Model Systems

A special issue of Insects (ISSN 2075-4450).

Deadline for manuscript submissions: 30 July 2026 | Viewed by 833

Special Issue Editors


E-Mail Website1 Website2
Guest Editor
1. Laboratorio de Insectos Sociales, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
2. Instituto de Fisiología, Biología Molecular y Neurociencias, UBA-CONICET, Buenos Aires 1428, Argentina
Interests: behavioral physiology; neurobiology; cognitive and chemical ecology; ecotoxicology; crop pollination; using honeybees, stingless bees, and bumblebees as model organisms

E-Mail Website
Guest Editor
Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil
Interests: stingless bee; biology; ecology; behavior; environmental physiology

Special Issue Information

Dear Colleagues,

There is an increasing number of experimental models among pollinating insects that enable the extrapolation of their responses from controlled laboratory conditions to natural environments. Assessing their cognitive and sensory abilities, as well as their physiological and genetic responses to quantifiable changes, provides valuable insights into how these beneficial insects might react to environmental fluctuations across habitats with different levels of disturbance.

This Special Issue gathers both experimental and field studies, focusing on conventional and non-conventional pollinating insect models, to explore the flexibility of their biological responses to environmental change.

Prof. Dr. Walter Marcelo Farina
Prof. Dr. Michael Hrncir
Guest Editors

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Keywords

  • native and introduced pollinators
  • bees
  • experimental biology
  • field-controlled assays

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Published Papers (1 paper)

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Research

13 pages, 1960 KB  
Article
Effect of Baicalin on the Proliferation of Nosema ceranae in Apis cerana
by Xu Han, Jin-Hua Xiao, Wu-Jun Jiang and Zhi-Jiang Zeng
Insects 2026, 17(5), 454; https://doi.org/10.3390/insects17050454 - 24 Apr 2026
Viewed by 479
Abstract
Nosema ceranae is a common and highly contagious fungal pathogen that primarily infects the gut of adult honeybees, causing nosemosis. As a chronic disease of the digestive system, it poses a global threat to honeybee health and colony sustainability. This study aimed to [...] Read more.
Nosema ceranae is a common and highly contagious fungal pathogen that primarily infects the gut of adult honeybees, causing nosemosis. As a chronic disease of the digestive system, it poses a global threat to honeybee health and colony sustainability. This study aimed to investigate the inhibitory effects of different concentrations of Scutellaria baicalensis aqueous extract on N. ceranae in the intestines of infected Apis cerana through feeding experiments. In addition, the therapeutic efficacy of its major active component, baicalin, was evaluated, and its potential molecular mechanisms of action were explored. The results showed that, compared with the control group, administration of S. baicalensis aqueous extract at concentrations of 1 mg/mL, 5 mg/mL, and 10 mg/mL significantly reduced midgut spore loads (p < 0.05). Further experiments showed that a 0.5 mg/mL baicalin sucrose solution, prepared with 0.5% (v/v) DMSO as co-solvent, exhibited optimal solubility and significantly inhibited the proliferation of spores in the honeybee midgut. Transcriptomic analysis of A. cerana revealed varying numbers of significantly differentially expressed genes among the baicalin-treated (HG) group, the co-solvent control (DMSO) group, and the blank control (C) group. Four candidate DEGs associated with the effects of baicalin were further identified, namely LOC108003965, LOC108000905, LOC107996681, and CYP4G11. Gene Ontology enrichment analysis showed that, in the comparison between the HG group and the C group, these DEGs were significantly enriched in six functional categories: iron ion binding, phosphoric ester hydrolase activity, heme binding, tetrapyrrole binding, hydrolase activity (acting on ester bonds), and oxidoreductase activity (acting on paired donors, with incorporation or reduction of molecular oxygen). Collectively, these results demonstrate that S. baicalensis aqueous extract effectively inhibits the proliferation of N. ceranae within the host, and its active component, baicalin, exhibits a similar inhibitory effect. The present study proposes a novel strategy in which baicalin may enhance host endogenous chitinase-related activity to target and disrupt the spore wall, offering a new perspective for the prevention and control of honeybee nosemosis. Full article
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