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New Advances in Animal Reproduction

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 10 August 2026 | Viewed by 1070

Special Issue Editor


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Guest Editor
Laboratório de Fisiologia Reprodutiva (FisioRep Lab), Universidade Federal do Delta do Parnaíba (UFDPar), Parnaíba 64202-020, PI, Brazil
Interests: ovarian physiology; preantral follicles; embryo; oocyte maturation; testicular physiology; spermatozoa; in vitro culture

Special Issue Information

Dear Colleagues,

Reproductive efficiency and fertility management remain central challenges in livestock production, wildlife populations, and human assisted reproduction. Recent advances in assisted reproductive technologies have expanded our understanding of key reproductive processes, including gametogenesis, embryo development, and reproductive physiology across multiple animal species, including humans. These developments not only improve reproductive outcomes but also enhance genetic preservation, animal welfare, and fertility preservation in oncology patients, among other applications.

We are pleased to invite you to contribute to this Special Issue, which aims to compile laboratory-based research and comprehensive reviews on the mechanisms, technologies, and applications driving advancements in animal reproduction. Submissions may propose innovative solutions or deepen the understanding of key challenges in reproductive biology and medicine, such as fertility preservation in oncology patients, improving reproductive efficiency, and enhancing reproductive conservation across species.

This Special Issue aims to highlight advances in animal reproduction across a broad range of species, including (but not limited to) cattle, goats, sheep and murine models.

In this Special Issue, original research articles and reviews are welcome. Research areas may include (but not limited to) the following:

  • Folliculogenesis
  • Spermatogenesis
  • Ovary and testis bioengineering
  • In vitro culture of gonadal tissue
  • Oocyte maturation
  • Embryo production

I look forward to receiving your contributions.

Dr. Valdevane Rocha Araújo
Guest Editor

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Keywords

  • folliculogenesis
  • oogenesis
  • oocyte maturation
  • follicle growth
  • preantral follicles
  • antral follicles
  • spermatogenesis
  • spermatozoa
  • bioengineering
  • scaffolds

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Published Papers (1 paper)

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Research

19 pages, 2055 KB  
Article
Punica granatum L. Modulates Antioxidant Activity in Vitrified Bovine Ovarian Tissue
by Solano Dantas Martins, Maria Alice Felipe Oliveira, Venância Antônia Nunes Azevedo, Francisco das Chagas Costa, Ingrid Gracielle Martins da Silva, Selene Maia de Morais, Sônia Nair Báo, José Roberto Viana Silva, Vânia Marilande Ceccatto and Valdevane Rocha Araújo
Int. J. Mol. Sci. 2026, 27(2), 903; https://doi.org/10.3390/ijms27020903 - 16 Jan 2026
Viewed by 654
Abstract
This study aimed to evaluate the effects of an ethanolic extract from Punica granatum L. (EE-PG) on bovine ovarian tissue vitrification, focusing on follicular morphology, ultrastructure, stromal cell density, collagen distribution, redox status, and mRNA expression of antioxidant-related genes. Bovine ovarian cortex fragments [...] Read more.
This study aimed to evaluate the effects of an ethanolic extract from Punica granatum L. (EE-PG) on bovine ovarian tissue vitrification, focusing on follicular morphology, ultrastructure, stromal cell density, collagen distribution, redox status, and mRNA expression of antioxidant-related genes. Bovine ovarian cortex fragments were divided into a fresh control group for in vivo tissue evaluation or vitrified either with the base vitrification solution (αMEM) alone or supplemented with different concentrations of EE-PG (10, 50, and 100 µg/mL), and subsequently stored in liquid nitrogen for 5 days. After warming, fragments were allocated for morphological and oxidative stress analyses or incubated for 24 h to resumption of cellular metabolism. The concentrations of 10 and 100 µg/mL preserved follicular morphology immediately after warming, and were therefore selected for ultrastructural evaluation. Both concentrations mitigated vitrification-induced damage. Gene expression analysis showed decreased levels of catalase (cat), Glutathione Peroxidase 1 (gpx1), and Nuclear Factor Erythroid 2-Related Factor 2 (nrf2) compared with the fresh control, whereas Superoxide Dismutase (SOD) enzymatic activity increased after incubation with 10 µg/mL EE-PG compared with all experimental groups. Moreover, Malondialdehyde (MDA) levels in tissues treated with 10 or 100 µg/mL were comparable to fresh controls after incubation. Overall, EE-PG at 10 or 100 µg/mL in the vitrification solution supported the maintenance of tissue morphology, redox balance—despite the downregulation of essential antioxidant genes, which may be associated with a reduced demand for enzymatic antioxidant defense—and cellular metabolism, indicating potential for improving bovine ovarian tissue vitrification outcomes. Full article
(This article belongs to the Special Issue New Advances in Animal Reproduction)
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