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Regenerative Applications of Stem Cells, PRP, Micrografts, and Biomaterials

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (1 April 2020) | Viewed by 14478

Special Issue Editor


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Guest Editor
Associate Professor of Plastic and Reconstructive Surgery, Surgical Science Department, University of Rome “Tor Vergata”, Rome, Italy
Interests: regenerative plastic surgery; adipose-derived mesenchymal stem cells; fat grafting; platelet-rich plasma; growth factors; mesenchymal stem cells; biomaterials; hair loss; androgenetic alopecia
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Special Issue Information

Dear Colleagues,

Tissue engineering and regenerative plastic surgery are interrelated terms that often go hand-in-hand when discussing stem cells (mesenchymal and follicular), biotechnology and bioactive molecules (platelet-rich plasma (PRP), micrografts), and biomaterials (scaffolds, hydrogels) for hair re-growth, fat graft improvement, and/or regeneration of tissues for biomedical applications (in relation to tissues of the face and body, cartilage, skin, and hair).

Basic research related to tissue regeneration, both in vitro and in vivo, will definitively contribute to the regenerative plastic surgery field.

In light of current knowledge on chondrocytes, epithelial and dermal cells, human follicle cells (HFSCs), and adipose-derived stem cells (ASCs) and their associations with biomaterials, numerous researchers have developed different strategies to improve the effects of these varied biotechnology applications.

This Special Issue aims to collect submissions of original research articles and reviews that demonstrate biomolecular pathways and effects of stem cells, PRP, micrografts, and biomaterials in regenerative plastic surgery.

Potential topics include, but are not limited to, the following:

  • Breast reconstruction with hybrid techniques, represented by prosthesis and biomaterials (mesh), analyzed by instrumental and clinical evaluations in patients with breast cancer and mastectomy outcomes;
  • Hair regrowth estimated by TrichoScan, hair dystrophy estimated by dermoscopy, and cell proliferation estimated by Ki67 assessment in patients affected by hair loss treated with micrografts, cells (follicular/ASC/SVF/epithelial/dermal), PRP, growth factors, and other biotechnologies;
  • Fat graft maintenance estimated by instrumental evaluation in patients with face and breast soft tissue defects treated with fat injections enriched with stem cells (ASC/SVF) and/or PRP;
  • Cartilage and chondrocytes in nose reconstruction estimated by instrumental and team evaluations in patients with minor soft tissue defects of the nose (valve collapse and pinch nose deformity) treated with cartilage paste grafting (diced cartilage, shaving cartilage, and crushing) and/or chondrocytes; and
  • Ulcer treatment performed with biomaterials/PRP/stem cells, estimated by instrumental and team evaluations.

Dr. Pietro Gentile
Guest Editor

Manuscript Submission Information

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Keywords

  • regenerative medicine
  • regenerative plastic surgery
  • adipose-derived stem cells
  • platelet-rich plasma
  • biomaterials
  • micrografts

Published Papers (2 papers)

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Research

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9 pages, 238 KiB  
Article
Impact of the Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma (A-PRP) and Activated Platelet-Rich Plasma (AA-PRP) in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation
by Pietro Gentile, Claudio Calabrese, Barbara De Angelis, Laura Dionisi, Jacopo Pizzicannella, Ashutosh Kothari, Domenico De Fazio and Simone Garcovich
Int. J. Mol. Sci. 2020, 21(2), 431; https://doi.org/10.3390/ijms21020431 (registering DOI) - 9 Jan 2020
Cited by 84 | Viewed by 7866
Abstract
Autologous therapies using platelet-rich plasma (PRP) need meticulous preparation—currently, no standardised preparation technique exists. Processing Quantitative Standards (PQSs) define manufacturing quantitative variables (such as time, volume and pressure). Processing Qualitative Standards (PQLSs) define the quality of the materials and methods of manufacturing. The [...] Read more.
Autologous therapies using platelet-rich plasma (PRP) need meticulous preparation—currently, no standardised preparation technique exists. Processing Quantitative Standards (PQSs) define manufacturing quantitative variables (such as time, volume and pressure). Processing Qualitative Standards (PQLSs) define the quality of the materials and methods of manufacturing. The aim of this review is to use existing PQSs and PQLs to report the in vivo/in vitro results obtained by using different Kits, that utilise different procedures (classified as Closed-Technique and Opened-Technique) to isolate autologous human activated (AA-PRP) or non-activated PRP (A-PRP). PQSs included the volumes of blood collected as well as the reagents used, the time/gravity of centrifugation, and the duration, temperature and tilt level/speed of centrifugation. PQLSs included the use of Calcium Chloride CaCl2, Kit weight, transparency of Kit components, the maintenance of a closed sterile processing environment and the use of a small centrifuge. Eight CE marked devices for PRP extraction were evaluated: Angel®, Biomed®, Cascade® and Selphyl®, Mag-18®, i-Stem®, MyCells® and Regenlab®. Using a Kit with the PQSs and PQLSs described in this study enables the isolation of A-PRP, thereby meeting consensus quality criteria. As our understanding of Critical Quality Attributes (CQAs) of A-PRP continues to evolve, especially with respect to purity and potency, adjustments to these benchmark PQSs and PQLs will hopefully help isolate A-PRP of desired CQAs with greater reproducibility, quality, and safety. Confirmatory studies will no doubt need to be completed. Full article

Review

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26 pages, 324 KiB  
Review
Impact of the Different Preparation Methods to Obtain Human Adipose-Derived Stromal Vascular Fraction Cells (AD-SVFs) and Human Adipose-Derived Mesenchymal Stem Cells (AD-MSCs): Enzymatic Digestion Versus Mechanical Centrifugation
by Pietro Gentile, Claudio Calabrese, Barbara De Angelis, Jacopo Pizzicannella, Ashutosh Kothari and Simone Garcovich
Int. J. Mol. Sci. 2019, 20(21), 5471; https://doi.org/10.3390/ijms20215471 - 2 Nov 2019
Cited by 78 | Viewed by 6114
Abstract
Autologous therapies using adipose-derived stromal vascular fraction (AD-SVFs) and adult adipose-derived mesenchymal stem cells (AD-MSCs) warrant careful preparation of the harvested adipose tissue. Currently, no standardized technique for this preparation exists. Processing quantitative standards (PQSs) define manufacturing quantitative variables (such as time, volume, [...] Read more.
Autologous therapies using adipose-derived stromal vascular fraction (AD-SVFs) and adult adipose-derived mesenchymal stem cells (AD-MSCs) warrant careful preparation of the harvested adipose tissue. Currently, no standardized technique for this preparation exists. Processing quantitative standards (PQSs) define manufacturing quantitative variables (such as time, volume, and pressure). Processing qualitative standards (PQLSs) define the quality of the materials and methods in manufacturing. The purpose of the review was to use PQSs and PQLSs to report the in vivo and in vitro results obtained by different processing kits that use different procedures (enzymatic vs. non-enzymatic) to isolate human AD-SVFs/AD-MSCs. PQSs included the volume of fat tissue harvested and reagents used, the time/gravity of centrifugation, and the time, temperature, and tilt level/speed of incubation and/or centrifugation. PQLSs included the use of a collagenase, a processing time of 30 min, kit weight, transparency of the kit components, the maintenance of a closed sterile processing environment, and the use of a small centrifuge and incubating rocker. Using a kit with the PQSs and PQLSs described in this study enables the isolation of AD-MSCs that meet the consensus quality criteria. As the discovery of new critical quality attributes (CQAs) of AD-MSCs evolve with respect to purity and potency, adjustments to these benchmark PQSs and PQLs will hopefully isolate AD-MSCs of various CQAs with greater reproducibility, quality, and safety. Confirmatory studies will no doubt need to be completed. Full article
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