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Protein Biosensors

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: 28 February 2025 | Viewed by 2005

Special Issue Editors


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Guest Editor
1. Institute of Food Science, CNR Italy, 83100 Avellino, Italy
2. URT-ISA, CNR at Department of Biology, University of Naples Federico II, 80126 Napoli, Italy
Interests: optical biosensors; fluorescence correlation spectroscopy; surface characterization

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Guest Editor
Institute of Food Science, CNR Italy, 83100 Avellino, Italy
Interests: fluorescence biosensors; molecular interactions; stable proteins

Special Issue Information

Dear Colleagues,

Protein-based biosensors represent one of the emerging methods for quickly analyzing the presence of target analytes in complex matrix samples. The use of proteins for the design of biosensors should ensure high selectivity and specificity. In fact, proteins are complex molecules that play many critical roles in cells. They are involved in most cellular functions, enabling the regular function of the cell. Protein-based biosensors can be constructed starting from a system with two nearly isoenergetic states, the equilibrium between which should be modulated by the binding of the target analyte. Desirable properties of proteins in such biosensors include: (a) conformational variations due to the binding of the target analyte should be large enough to be detected by the operator; (b) the system should be tunable so that target analytes with different concentrations can be detected over a large dynamic range; (c) conformational variations should be coupled to a sensitive output; and (d) the proteins should be stable in operative conditions under which biosensors should work.

Dr. Antonio Varriale
Dr. Maria Staiano
Guest Editors

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Published Papers (1 paper)

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Research

10 pages, 1706 KiB  
Communication
Difunctional Magnetic Nanoparticles Employed in Immunochromatographic Assay for Rapid and Quantitative Detection of Carcinoembryonic Antigen
by Yalin Hu, Xin Lu, Liyue Shen, Jiahui Dong, Zhanwei Liang, Jie Xie, Tao Peng, Xiaoping Yu and Xinhua Dai
Int. J. Mol. Sci. 2023, 24(16), 12562; https://doi.org/10.3390/ijms241612562 - 8 Aug 2023
Cited by 1 | Viewed by 1498
Abstract
Immunochromatographic assay (ICA) plays an important role in in vitro diagnostics because of its simpleness, convenience, fastness, sensitivity, accuracy, and low cost. The employment of magnetic nanoparticles (MNPs), possessing both excellent optical properties and magnetic separation functions, can effectively promote the performances of [...] Read more.
Immunochromatographic assay (ICA) plays an important role in in vitro diagnostics because of its simpleness, convenience, fastness, sensitivity, accuracy, and low cost. The employment of magnetic nanoparticles (MNPs), possessing both excellent optical properties and magnetic separation functions, can effectively promote the performances of ICA. In this study, an ICA based on MNPs (MNP–ICA) has been successfully developed for the sensitive detection of carcinoembryonic antigen (CEA). The magnetic probes were prepared by covalently conjugating carboxylated MNPs with the specific monoclonal antibody against CEA, which were not only employed to enrich and extract CEA from serum samples under an external magnetic field but also used as a signal output with its inherent optical property. Under the optimal parameters, the limit of detection (LOD) for qualitative detection with naked eyes was 1.0 ng/mL, and the quantitative detection could be realized with the help of a portable optical reader, indicating that the ratio of optical signal intensity correlated well with CEA concentration ranging from 1.0 ng/mL to 64.0 ng/mL (R2 = 0.9997). Additionally, method comparison demonstrated that the magnetic probes were beneficial for sensitivity improvement due to the matrix effect reduction after magnetic separation, and the MNP–ICA is eight times higher sensitive than ICA based on colloidal gold nanoparticles. The developed MNP–ICA will provide sensitive, convenient, and efficient technical support for biomarkers rapid screening in cancer diagnosis and prognosis. Full article
(This article belongs to the Special Issue Protein Biosensors)
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