ijms-logo

Journal Browser

Journal Browser

Application of NMR Spectroscopy in Biomolecules: 2nd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biophysics".

Deadline for manuscript submissions: 31 October 2026 | Viewed by 600

Special Issue Editor


E-Mail Website
Guest Editor
Department of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania Luigi Vanvitelli, Via Antonio Vivaldi 43, 81100 Caserta, Italy
Interests: nuclear magnetic resonance spectroscopy; protein structure and dynamics; protein folding and misfolding; metal-binding protein; conformational equilibria; neurodegenerative diseases
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Nuclear magnetic resonance spectroscopy is a powerful biophysical technique for studying the structure, dynamics, folding mechanisms, and interactions of biomolecules at an atomic resolution. This Special Issue is dedicated to new advances in the technical developments and applications of solution and solid-state NMR methodologies in biology, pathology, and pharmacology covering structural biology (i.e., proteins, peptides, nucleic acids, and carbohydrates; membrane proteins and lipids); biomolecule interactions (i.e., protein–protein, protein–DNA, antigen–antibody); folding and disordered proteins; conformational dynamics; molecular mechanisms underlying disease progression; structural and dynamical features of biomolecules in cellular environments; and pharmaceutical applications (i.e., structure/fragment-based drug discovery and metabolomics). In this regard, I would be very pleased if you would agree to contribute to this Special Issue either with an original research article, a short communication, or a review.    

I am also more than honored to announce that the Topical Advisory Panel Member Dr. Gianluca D'Abrosca (from the University of Foggia, Italy) will be participating in this Special Issue.

Dr. Luigi Russo
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • advanced solution and solid state biomolecular NMR techniques
  • structure and dynamics of biomolecules: proteins, peptides, and nucleic acids
  • paramagnetic NMR
  • in/on cell-NMR
  • protein–ligand, protein–DNA, protein–RNA interaction
  • protein folding and misfolding
  • structure-based drug screening, fragment-based drug screening
  • computational NMR methods
  • sample preparation and isotope labeling strategies
  • NMR metabolomics and foodomics
  • membrane proteins and lipids
  • natural-abundance NMR spectroscopy
  • pharmaceutical NMR applications
  • disordered proteins and peptides
  • drugs development
  • metal-binding biomolecules
  • hyperpolized NMR techniques
  • 19F NMR spectroscopy
  • NMR techniques for the analysis of complex mixtures
  • integrated NMR-based methodologies

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Review

52 pages, 1516 KB  
Review
Multinuclear NMR and MRI Beyond Proton Imaging: Principles, Contrast Mechanisms, and Applications in Materials and Biomedicine
by Dorota Bartusik-Aebisher, Klaudia Dynarowicz, Barbara Smolak, Rostyslav Marunych, Wiesław Guz and David Aebisher
Int. J. Mol. Sci. 2026, 27(10), 4384; https://doi.org/10.3390/ijms27104384 - 14 May 2026
Viewed by 122
Abstract
Magnetic resonance techniques have evolved beyond conventional proton-based imaging, enabling access to a broader range of nuclei that provide complementary structural, functional, and molecular information. This review presents a comprehensive overview of multinuclear NMR and MRI in solid and soft materials as well [...] Read more.
Magnetic resonance techniques have evolved beyond conventional proton-based imaging, enabling access to a broader range of nuclei that provide complementary structural, functional, and molecular information. This review presents a comprehensive overview of multinuclear NMR and MRI in solid and soft materials as well as in biomedical applications, with particular emphasis on 1H, 13C, 31P, 23Na, and 19F nuclei. Proton-based methods remain the foundation of magnetic resonance due to their high sensitivity and widespread applicability, offering insights into molecular mobility, hydration, and microstructural heterogeneity. In contrast, heteronuclear approaches enable more specific characterization of chemical structure (13C), phosphorus-containing functional groups and membranes (31P), ionic homeostasis and transport (23Na), and exogenous tracers with negligible biological background (19F). Together, these techniques extend magnetic resonance from primarily anatomical imaging toward functional, metabolic, and molecular-level analysis. The review further discusses key hardware aspects, including magnetic field strength and radiofrequency coil design, highlighting the trade-offs between low- and high-field systems and the growing importance of multinuclear coil architectures. For example, because 1H, 23Na, 31P, and 19F resonate at different Larmor frequencies, multinuclear experiments require dedicated or multi-tuned RF coils that balance sensitivity, field homogeneity, and decoupling between channels. Mechanisms of contrast generation are examined in detail, distinguishing between endogenous sources—such as water, ions, and metabolites—and exogenous contrast agents, including gadolinium-, manganese-, and fluorine-based compounds, as well as targeted and theranostic platforms. A comparative framework of endogenous and exogenous signals is presented, emphasizing their complementary roles in balancing safety, specificity, and sensitivity. Finally, the opportunities and challenges of multinuclear magnetic resonance are critically evaluated, including limitations in sensitivity, signal-to-noise ratio, data interpretation in heterogeneous systems, and technical complexity. Emerging directions such as ultrahigh-field imaging, advanced RF technologies, hyperpolarization, and artificial intelligence-assisted reconstruction are discussed as key drivers for future development. Overall, multinuclear NMR and MRI represent a powerful and expanding toolbox for probing complex material and biological systems, with the potential to significantly enhance diagnostic capabilities and deepen our understanding of structure–function relationships across multiple scales. Full article
(This article belongs to the Special Issue Application of NMR Spectroscopy in Biomolecules: 2nd Edition)
Back to TopTop