Production in Forest Nurseries, Field Performance of Seedlings and Natural Regeneration in the Context of Climate Change: 2nd Edition

A special issue of Forests (ISSN 1999-4907). This special issue belongs to the section "Forest Ecology and Management".

Deadline for manuscript submissions: closed (28 February 2026) | Viewed by 1524

Special Issue Editors


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Guest Editor
Faculty of Forestry, Geography and Geomatics, Abitibi Price Building, Laval University, Quebec, QC G1V 0A6, Canada
Interests: forest nursery; ecophysiology; cultural practices; composting and growing media; environmental stress; vegetative propagation; genetic of ectomycorrhizal fungi; assisted migration; heavy metal tolerance; seedling performance
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Centre de Recherche et d’Innovation sur les Végétaux, Faculté des Sciences de l’Agriculture et de l’Alimentation, Université Laval, 2480 boul., Hochelaga, Quebec, QC G1V 0A6, Canada
Interests: tree physiology; gas exchange; soil–plant interactions; environmental biophysics; irrigation; microclimate; peat substrate properties
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Centre for Forest Research and Institute for Systems and Integrative Biology, Université Laval, 1030 Avenue de la Médecine, Quebec, QC G1V 0A6, Canada
Interests: biotechnologies of mycorrhizal and nitrogen-fixing symbioses; nursery production; molecular ecology and environmental genomics; agroforestry; mining restoration; invasive plant biology; field performance
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The success of reforestation and restoration programs concerns forest land managers worldwide. With climatic extremes and unpredictable increases in natural disturbances (fires, insect attacks, etc.) across millions of hectares, forest managers are must seek to maintain biodiversity and increase forest productivity by combining the use of reforestation and natural regeneration.

The success of the various reforestation programs (including those aimed at production, protection, recreation, agroforestry, combating desertification, etc.) is a major challenge that requires optimization and control of the various steps in the production chain of seedlings of high morphophysiological quality, from seeds to cuttings and emblings to planting. The establishment, survival, and growth of seedlings in reforestation sites are factors closely linked to the different cultural practices in nurseries and silvicultural techniques in reforestation sites. In the context of climate change, seedlings and plantations are subject to different environmental stresses and a variety of very severe climatic extremes that negatively affect their survival and growth.

Thus, the morphophysiological quality of seedlings is one of the essential components that directly affects the success of reforestation programs. The production of quality seedlings or emblings in forest nurseries and the success of plantations are strongly linked to the selection of genetic sources (provenance, families, clones) adapted to the plantation sites, to the different cultural practices applied in the nursery, to the handling, transportation, and storage of seedlings or emblings before their plantation on a reforestation site, to site preparation, and to silvicultural techniques.

In addition to reforestation (artificial regeneration), it is necessary to understand natural regeneration patterns as well as the development of methods, silvicultural techniques, and tools to improve the success of the renewal of the stands in response to increasing biotic and abiotic stresses (drought, fires, insect and fungal attacks, etc.). 

This Special Issue will integrate original research, practical strategies, and reviews related to, but not limited to, the following potential topics:

(a) Genetic material and seed quality, growth and physiology of seedlings produced by somatic embryogenesis, cutting, seeds, and other propagation techniques;

(b) Production in forest nurseries and effects of different practical techniques on the morphophysiological quality of tree and agroforestry seedlings or emblings: containers, and seedling size, physicochemical properties of different growing media, management of water and fertilizers, mineral nutrient leaching, water stress preconditioning, ecophysiology of seedlings or stecklings, frost and water stress tolerance, short-day treatment, hardening and dehardening; seedling or embling storage; mycorrhizal fungi and other symbioses, etc.;

(c) Field performance of tree and agroforestry seedlings or emblings under different site conditions, handling and transportation, assignment of the genetic characteristics of seedlings to the conditions of the planting site and the current and future climate, assisted migration, competing vegetation, site preparation techniques and silvicultural treatments, growth and physiology of seedlings or emblings in response to different environmental stress conditions (drought, salinity, heavy metal, frost, etc.);

(d) The development of operational research and development programs adapted to the production chain of tree seedlings and the transfer of knowledge to practitioners in developed and developing countries;

(e) Natural regeneration in response to increasing biotic and abiotic stresses (drought, fires, insect and fungal attacks, etc.), factors affecting natural regeneration, ecophysiology of natural regeneration, silvicultal, silvicultural techniques to facilitate and improve the establishment, survival and growth of natural regeneration, etc. 

Dr. Mohammed S. Lamhamedi
Prof. Dr. Steeve Pepin
Prof. Dr. Damase P. Khasa
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Forests is an international peer-reviewed open access monthly journal published by MDPI.

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Keywords

  • seed and seedling quality
  • cutting
  • somatic embryogenesis
  • nursery production
  • cultural practices
  • environmental stress
  • mycorrhizal and bacteria symbioses
  • field performance
  • research and development programs adapted to the production chain of tree seedlings
  • agroforestry products and their valorization
  • natural regeneration

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Published Papers (2 papers)

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Research

18 pages, 38735 KB  
Article
Regeneration of Pyrophilic Sand Pine (Pinus clausa (Chapm. ex Engelm.) Vasey ex Sarg.) in Fragmented Fire-Suppressed Scrub, South Florida, USA
by George Rogers
Forests 2026, 17(4), 504; https://doi.org/10.3390/f17040504 - 19 Apr 2026
Viewed by 159
Abstract
Pinus clausa var. clausa (Chapm. ex Engelm.) Vasey ex Sarg., sand pine, is the dominant tree of biorich but ecologically compromised Southeast Florida scrub. Scrub habitats and P. clausa have dwindled due to habitat reduction and fragmentation, regional development, and fire suppression. The [...] Read more.
Pinus clausa var. clausa (Chapm. ex Engelm.) Vasey ex Sarg., sand pine, is the dominant tree of biorich but ecologically compromised Southeast Florida scrub. Scrub habitats and P. clausa have dwindled due to habitat reduction and fragmentation, regional development, and fire suppression. The purpose of the present article was to seek correlates of P. clausa establishment under present unnatural development-impacted conditions using 428 field measurements at four sites to determine spatial positioning preferences relative to vegetation edges, then adding 120 measurements at a single site aimed at evaluating several potential predictors of P. clausa establishment. Potential establishment predictors were adjacency to other woody plants, depth to hard sand horizon, seed tree distance and direction, light-intensity, soil-core color, soil pH and soil surface firmness. Comparing frequency distributions of juvenile P. clausa locations with frequency distributions of random spots within the same perimeters, juvenile pines tended toward adjacency to other woody plants (chi2 p < 0.0001), toward shallow hard horizons (Kolmogorov–Smirnov p = 0.0006), toward soft soil surfaces (K–S p = 0.007), and toward proximity to seed trees (K–S p = 0.004). Additionally, juvenile P. clausa were often clustered under groves of Quercus geminata Small with comparatively thin canopies. Bayesian logistic regression showed adjacency to woody plants as a strong predictor of P. clausa establishment. When alongside other plants, P. clausa establishment was mostly on the north or east side of neighboring plant edges. Overall conclusions were that juvenile Pinus clausa in SE Florida scrub fragments is sensitive to positioning relative to other woody plants, and is associated with soil surface softness, soil depth to hard horizon, and light levels, except as seedlings. Full article
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17 pages, 10102 KB  
Article
Evaluating the Effects of Glutathione and Controlled-Release Fertilizer on the Height Growth of Cryptomeria japonica D. Don Seedlings Accounting for Topography and Vegetation
by Hisanori Harayama, Shiro Okuda, Hiromi Yamagawa, Takami Saito, Daisuke Kabeya and Hiroyuki Tobita
Forests 2025, 16(9), 1407; https://doi.org/10.3390/f16091407 - 2 Sep 2025
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Abstract
Fertilization is occasionally applied to promote early growth of outplanted tree seedlings. However, the effectiveness of fertilization can be obscured by topographic variations and competing vegetation. The aim of this study was to reevaluate the effects of fertilization and glutathione disulfide (GSSG) on [...] Read more.
Fertilization is occasionally applied to promote early growth of outplanted tree seedlings. However, the effectiveness of fertilization can be obscured by topographic variations and competing vegetation. The aim of this study was to reevaluate the effects of fertilization and glutathione disulfide (GSSG) on Cryptomeria japonica D. Don (Japanese cedar) seedling height using a four-year dataset from a previous study showing no significant effects using linear models. The impact of treatment was examined using random forest, generalized additive models (GAMs), and structural equation models (SEMs), while accounting for topography and competing vegetation. Topographic features, including the topographic wetness index, were the primary determinants of height growth, reflecting C. japonica’s preference for moist environments. Although the effects of fertilization and GSSG were limited, the GAMs indicated marginal positive interactions in specific stable topographic contexts. The SEMs revealed that fertilization and GSSG indirectly negatively reduced height by increasing competing vegetation coverage. By applying these advanced statistical approaches, we demonstrate how treatment effects that conventional analyses might overlook can be detected, illustrating the methodological contribution of this study. These findings show that topography plays a dominant role in early C. japonica growth, and fertilization and GSSG provide only modest, context-dependent benefits. Full article
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