Yeasts as Microbial Cell Factories: Diversity, Biotechnology Potential and Applications

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Yeast".

Deadline for manuscript submissions: 15 March 2026 | Viewed by 834

Special Issue Editor


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Guest Editor
Department of Biotechnology, Microbiology and Food Evaluation, Warsaw University of Life Sciences, Warsaw, Poland
Interests: food biotechnology; microbial isolation; microbial lipid biosynthesis; carotenoids produced by yeasts; biotechnological valorization of industrial waste

Special Issue Information

Dear Colleagues,

Yeasts are metabolically versatile microorganisms that are of great relevance for industrial biotechnology and, beyond their traditional roles in food and beverage fermentation, both conventional and non-conventional yeasts are increasingly recognized as efficient microbial cell factories for the production of high-value compounds such as lipids, carotenoids, enzymes, organic acids, and bioethanol.

This Special Issue aims to highlight recent advances in yeast biodiversity, functional properties, metabolic engineering, and bioprocess optimization, and topics of interest include the discovery of novel yeast species, the probiotic potential of yeasts, their interactions in co-cultivation systems, their applications in food and health sectors, and the sustainable biotechnological use of industrial residues. Both original research and review articles are welcome.

Dr. Anna Maria Kot
Guest Editor

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Keywords

  • yeast biodiversity
  • non-conventional yeasts
  • fermentation
  • functional metabolites
  • food biotechnology
  • microbial waste valorization
  • bioprocess optimization

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Published Papers (1 paper)

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Research

20 pages, 1067 KB  
Article
A Potential of Agro-Industrial Biowaste as Low-Cost Substrates for Carotenoid Production by Rhodotorula mucilaginosa
by Olja Šovljanski, Dragoljub Cvetković, Tara Budimac, Anja Vučetić, Ana Tomić, Teodora Marić and Aleksandra Ranitović
Fermentation 2025, 11(9), 531; https://doi.org/10.3390/fermentation11090531 - 12 Sep 2025
Viewed by 723
Abstract
The sustainable production of natural pigments is gaining attention as industries seek alternatives to synthetic additives. This study explored agro-industrial biowastes as feedstocks for carotenoid biosynthesis by Rhodotorula mucilaginosa (natural isolate from Jerusalem artichoke), aiming to identify an optimal substrate that combines high [...] Read more.
The sustainable production of natural pigments is gaining attention as industries seek alternatives to synthetic additives. This study explored agro-industrial biowastes as feedstocks for carotenoid biosynthesis by Rhodotorula mucilaginosa (natural isolate from Jerusalem artichoke), aiming to identify an optimal substrate that combines high productivity with economic and environmental feasibility. Thirteen biowastes, including grape pomace, crude glycerol, chicken feathers, sugar beet juice, and pea protein isolate, were systematically evaluated for their impact on yeast growth and pigment accumulation. Carotenoid yields ranged from 21.4 to 187.2 mg/100 g dry weight, with the highest volumetric productivity achieved in pea protein isolate (14.98 mg/L), untreated white grape pomace (14.09 mg/L), and crude glycerol (13.87 mg/L). To assess scalability, a simplified techno-economic and sustainability analysis was applied, revealing that although pea protein isolate offered the best yields, its high market cost limited industrial feasibility. In contrast, untreated grape pomace and crude glycerol emerged as low-cost, abundant alternatives with strong circular bioeconomy potential. Fed-batch bioreactor validation using untreated grape pomace confirmed its suitability, achieving a 43% improvement in carotenoid productivity (20.1 mg/L) compared to shake-flask trials. These results position untreated grape pomace as the optimal substrate–strategy combination for sustainable carotenoid production linking agro-waste valorization with high-value bioproduct generation. This study provides both experimental evidence and economic rationale for integrating winery residues into industrial pigment production chains, advancing yeast biotechnology toward more circular and resource-efficient models. Full article
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