Bulk Protein Crystallization
Deadline for manuscript submissions: closed (21 May 2021) | Viewed by 9541
Interests: crystallization/precipitation processes; protein crystallization; calcium phosphates for biomedical applications; multiphase reactors; materials characterization
In the last decade, protein-based therapy has emerged as a safe and powerful tool for the administration of biopharmaceuticals. However, its manufacturing process, in particular the purification stage, remains costly. Unlike conventional protein purification techniques (i.e., chromatography), protein crystallization requires no costly equipment or consumables (e.g., resins) and can yield highly concentrated slurries of pure protein ready for further formulation in a single step.
Although its viability has been demonstrated, protein crystallization remains a largely empirical process due to the complexity of the underlying mechanisms and the unavailability of generalized crystallization strategies and scale-up criteria.
This is the motivation for this Special Issue, "Bulk Protein Crystallization ", which will gather the latest achievements in the search for better control and more rational design of protein crystallization processes. To this is added the recent advances in in situ monitoring and modelling. Studies on the influence of fluid dynamics on the process and crystal quality as well as on the specific crystallization of a given protein from a more realistic matrix (e.g., protein mixtures, clarified concentrated fermentation broths) are also crucial.
With the contribution of experts from chemical engineering, biotechnology and biochemistry, mathematics, and physics, this Special Issue is intended to contribute with robust and scalable protein crystallization strategies to boost its implementation in the pharmaceutical and biotechnological industry.
Reports on the following topics are welcome:
- Strategies to enhance the crystallization efficiency and decrease the process time;
- Strategies to enable the rapid establishment of phase diagrams;
- Advanced imaging, spectroscopy, and light scattering techniques;
- Modelling of protein crystallization processes (population balance modelling, etc.);
- Crystallization from “impure” protein solutions;
- Influence of hydrodynamics (mixing, shear, etc.) on protein crystallization.
Dr. Filipa Castro
Prof. Dr. Matthias Kind
Manuscript Submission Information
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- Protein crystallization
- Bioseparation/downstream processing
- Systematic crystallization strategies
- In situ monitoring