Advances of Genetically Encoded Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: closed (31 October 2021) | Viewed by 5319

Special Issue Editor


E-Mail Website
Guest Editor
Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Interests: protein engineering; near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs); genetically encoded biosensors and optogenetic tools
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Genetically Encoded Biosensors (GEBs) have revolutionized modern science. It was always a challenge for scientists to sense and measure biochemical changes within a cellular compartment or within a specific cell type in a transgenic organism without perturbing the metabolism of the experimental subject. After the discovery of fluorescent and bioluminescent proteins, as well as bringing microscopy and directed molecular evolution techniques to a new level, the development of advanced genetically encoded biosensors became possible. Scientists from around the world have put tremendous effort into the creation of the GEBs that measure concentrations and activity levels of molecules or ions. Steadily, the research arrives to a point when genetically encoded biosensors will be routinely used to monitor long-term signaling processes during the development, disease progression, and aging of a transgenic organism.

We encourage you to share your findings and ideas in the field of genetically encoded biosensors. This Special Issue will cover all possible types of current GEBs, challenges in their design, a plethora of their applications across scales, their multiplexing, tools required to work with GEBs, and future outlooks. In light of the COVID-19 pandemic, researchers are welcome to share their ideas and discoveries on how genetically encoded biosensors could be used in drug discovery.

Dr. Mikhail Baloban
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genetically encoded biosensors (GEBs)
  • fluorescent proteins (FPs)
  • bioluminescent proteins (BPs)
  • sensing unit (SU)

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

11 pages, 1687 KiB  
Article
FLIM-Based Intracellular and Extracellular pH Measurements Using Genetically Encoded pH Sensor
by Alexander S. Goryashchenko, Alexey A. Pakhomov, Anastasia V. Ryabova, Igor D. Romanishkin, Eugene G. Maksimov, Alexander N. Orsa, Oxana V. Serova, Andrey A. Mozhaev, Margarita A. Maksimova, Vladimir I. Martynov, Alexander G. Petrenko and Igor E. Deyev
Biosensors 2021, 11(9), 340; https://doi.org/10.3390/bios11090340 - 15 Sep 2021
Cited by 15 | Viewed by 4302
Abstract
The determination of pH in live cells and tissues is of high importance in physiology and cell biology. In this report, we outline the process of the creation of SypHerExtra, a genetically encoded fluorescent sensor that is capable of measuring extracellular media pH [...] Read more.
The determination of pH in live cells and tissues is of high importance in physiology and cell biology. In this report, we outline the process of the creation of SypHerExtra, a genetically encoded fluorescent sensor that is capable of measuring extracellular media pH in a mildly alkaline range. SypHerExtra is a protein created by fusing the previously described pH sensor SypHer3s with the neurexin transmembrane domain that targets its expression to the cytoplasmic membrane. We showed that with excitation at 445 nm, the fluorescence lifetime of both SypHer3s and SypHerExtra strongly depend on pH. Using FLIM microscopy in live eukaryotic cells, we demonstrated that SypHerExtra can be successfully used to determine extracellular pH, while SypHer3s can be applied to measure intracellular pH. Thus, these two sensors are suitable for quantitative measurements using the FLIM method, to determine intracellular and extracellular pH in a range from pH 7.5 to 9.5 in different biological systems. Full article
(This article belongs to the Special Issue Advances of Genetically Encoded Biosensors)
Show Figures

Figure 1

Back to TopTop