Micro and Nano Optic-Based Biosensing Technologies and Strategies—2nd Edition

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 20 June 2026 | Viewed by 767

Special Issue Editor


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Guest Editor
State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China
Interests: plasmonic nano-optics devices; optical fiber sensor; optical super-resolution imaging
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Special Issue Information

Dear Colleagues,

With the success of the first edition of this Special Issue, ‘Micro and Nano Optic-Based Biosensing Technologies and Strategies’, we are launching a second edition under the same title, aiming to continue to delve into the latest advancements in micro and nano optic-based biosensors across a variety of emerging applications, emphasizing their roles in biomedical imaging, biomolecule detection, environmental monitoring, and food safety testing.

This second edition aims to expand the previosu discussion in regard to several critical topics, including the following:

  1. Innovative sensing materials: Highlighting the development of new optical materials and nanostructures that enhance the sensitivity and specificity of biosensors, enabling the more accurate detection and analysis of target biomolecules.
  2. Integration with advanced technologies: Exploring the integration of micro–nano optic sensors with cutting-edge technologies such as electrochemical technologies, immunoassays, microfluidics, artificial intelligence, and machine learning, which can significantly improve data analysis and operational efficiency.
  3. Real-world applications: Examining the practical applications of micro–nano optic biosensors in various fields, including medical diagnostics, environmental protection, and food quality assurance, showcasing their effectiveness and real-life impacts.
  4. Challenges and future perspectives: Addressing the current challenges facing micro–nano optic biosensing technology, such as design ideas, latest development trends, and challenges, while discussing the potential future directions and innovations that can overcome these barriers.

We invite contributions from researchers and practitioners in the form of original research articles, reviews, or communications that advance the field of micro–nano optic-based biosensing.

Prof. Dr. Wenchao Zhou
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

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Keywords

  • micro/nano optics biosensors
  • microfluidics
  • lab-on-a-chip
  • Point-of-Care tests
  • SPR
  • optical micro/nano fibers
  • metasurface
  • nanophotonics

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Published Papers (1 paper)

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Review

30 pages, 2370 KB  
Review
Nanobiosensors for Single-Molecule Diagnostics: Toward Integration with Super-Resolution Imaging
by Seungah Lee, Sobia Rafiq and Seong Ho Kang
Biosensors 2025, 15(10), 705; https://doi.org/10.3390/bios15100705 - 21 Oct 2025
Viewed by 654
Abstract
Recent advances in nanotechnology and optical imaging have transformed molecular diagnostics, enabling the detection and analysis of individual biomolecules with unprecedented precision. Nanobiosensors provide ultrasensitive molecular detection, and super-resolution microscopy (SRM) exceeds the diffraction limit of conventional optics to achieve nanometer-scale resolution. Although [...] Read more.
Recent advances in nanotechnology and optical imaging have transformed molecular diagnostics, enabling the detection and analysis of individual biomolecules with unprecedented precision. Nanobiosensors provide ultrasensitive molecular detection, and super-resolution microscopy (SRM) exceeds the diffraction limit of conventional optics to achieve nanometer-scale resolution. Although their integration remains in its infancy, with only a handful of proof-of-concept studies reported, the convergence of nanobiosensors and SRM holds significant promise for next-generation diagnostics. In this review, we first outline nanobiosensor-based single-molecule detection strategies and highlight representative implementations. These include plasmonic–SRM hybrids, electrochemical–optical correlatives, and SRM-enabled immunoassays, with a focus on their applications in oncology, infectious diseases, and neurodegenerative disorders. Then, we discuss emerging studies at the interface of nanobiosensors and SRM, including nanostructure-assisted SRM. Despite not being true biosensing approaches, these studies provide valuable insights into how engineered nanomaterials can improve imaging performance. Finally, we evaluate current challenges, including reproducibility, multiplexing, and clinical translation, and outline future opportunities, such as the development of photostable probes, artificial intelligence-assisted image reconstruction, microfluidic integration, and regulatory strategies. This review highlights the synergistic potential of nanobiosensors and SRM, outlining a roadmap toward clinically translatable next-generation single-molecule diagnostic platforms. Full article
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