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Biosensors
Special Issues
Probes for Biosensing and Bioimaging
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Probes for Biosensing and Bioimaging

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: 31 May 2025 | Viewed by 10760

Special Issue Editors

Dr. Hongwen Liu

E-Mail Website
Guest Editor
College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, China
Interests: fluorescent probe; in situ imaging; signal amplification
Dr. Zhiqiang Mao

E-Mail Website
Guest Editor
Department of Chemistry, Hubei University, Wuhan, China
Interests: fluorescent probe; molecular theranostics; in vivo imaging

Special Issue Information

Dear Colleagues,

With the continuous development of biosensing and bioimaging technologies, fluorescent probes with the advantages of high sensitivity, selectivity, rapid responses and real-time detection have achieved remarkable achievements in food safety, environmental monitoring, disease diagnosis, and so on, and have exhibited great potential for various applications. The use of molecular fluorescent probes to monitor life activity events is of great importance to gain insight into relevant physiological and pathological processes, disease diagnosis and treatment. Molecular probe-based fluorescence imaging technology has become a powerful tool for monitoring disease biomarkers. It is believed that more biosensing and bioimaging probes will be taken from the laboratory to the practical field. Therefore, this Special Issue is dedicated to gathering significant advances in innovative research on the various probes available for biosensing and bioimaging and their various applications.

Dr. Hongwen Liu
Dr. Zhiqiang Mao
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescent probe
  • small-molecular probe
  • biosensing
  • bioimaging

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (5 papers)

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Research

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10 pages, 1706 KiB  
Article
MNAzyme-Assisted Nucleic Acid Lateral Flow Assay for Cost-Effective, On-Site Mercury Detection
by Seok Hyeon Kim, Yujun Kim, Seokjoon Kim, Eun Sung Lee, Byung Seok Cha and Ki Soo Park
Biosensors 2024, 14(10), 454; https://doi.org/10.3390/bios14100454 - 25 Sep 2024
Viewed by 1466
Abstract
Mercury ions (Hg2+) are toxic heavy metals present in the environment that pose significant health risks. An advanced detection system could allow for a prompt response and alleviate serious damage to humans. In this study, we developed a cost-effective, on-site detection [...] Read more.
Mercury ions (Hg2+) are toxic heavy metals present in the environment that pose significant health risks. An advanced detection system could allow for a prompt response and alleviate serious damage to humans. In this study, we developed a cost-effective, on-site detection method for Hg2+ using a multicomponent nucleic acid enzyme (MNAzyme)-assisted nucleic acid lateral flow assay (NALFA). The MNAzyme, which was engineered to contain thymine–thymine mismatches, is responsive only to the presence of Hg2+ and exerts efficient cleavage activity on substrates that can be captured by the NALFA strip, and thus the proposed system enables the visual detection of Hg2+ in the NALFA strip. Our assay demonstrated sufficient detection sensitivity and specificity to meet the WHO standards, offering a good practical alternative for rapid environmental and public health monitoring. Full article
(This article belongs to the Special Issue Probes for Biosensing and Bioimaging)
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14 pages, 5432 KiB  
Article
Mitochondrial Labeling with Mulberrin-Cy3: A New Fluorescent Probe for Live Cell Visualization
by Gangxiang Yuan, Yiwei Luo, Peng Qian and Ningjia He
Biosensors 2024, 14(9), 428; https://doi.org/10.3390/bios14090428 - 5 Sep 2024
Cited by 1 | Viewed by 2297
Abstract
Mitochondria, crucial intracellular organelles, are central to energy metabolism, signal transduction, apoptosis, calcium homeostasis, and a myriad of other biological processes, making them a focal point in diverse research fields. The capacity to fluorescently label and visually track mitochondria is crucial for understanding [...] Read more.
Mitochondria, crucial intracellular organelles, are central to energy metabolism, signal transduction, apoptosis, calcium homeostasis, and a myriad of other biological processes, making them a focal point in diverse research fields. The capacity to fluorescently label and visually track mitochondria is crucial for understanding their biological roles. We present mulberrin-Cy3, a novel small molecule fluorescent probe that selectively labels mitochondria in animal cells, including cancer cells, with relative ease. This protocol details the synthesis of mulberrin-Cy3 and its use for visualizing mitochondria in living cells. The synthesis is straightforward and time-efficient, and the labeling method is more accessible than traditional approaches, providing a cost-effective option for mitochondrial visualization at room temperature. The labeling is rapid, with effective labeling achieved within 5 min of incubation. The fluorescent signal is stable and brighter, offering a significant advantage over existing methods. Mulberrin-Cy3 represents a promising mitochondrial labeling compound, providing researchers with a novel experimental tool to explore the complex biological functions of mitochondria. This innovation has the potential to significantly advance our comprehension of mitochondrial dynamics and their role in cellular health and disease. Full article
(This article belongs to the Special Issue Probes for Biosensing and Bioimaging)
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9 pages, 2365 KiB  
Article
Development of a NIR Iridium(III) Complex-Based Probe for the Selective Detection of Iron(II) Ions
by Wanyi Wang, Zixi Zhang, Jingqi Liu, Lingtan Kong, Wanhe Wang, Chung-Hang Leung and Jing Wang
Biosensors 2024, 14(8), 369; https://doi.org/10.3390/bios14080369 - 29 Jul 2024
Viewed by 1507
Abstract
As a commonly used metal ion, iron(II) (Fe2+) ions pose a potential threat to ecosystems and human health. Therefore, it is particularly important to develop analytical techniques for the rapid and accurate detection of Fe2+ ions. However, the development of [...] Read more.
As a commonly used metal ion, iron(II) (Fe2+) ions pose a potential threat to ecosystems and human health. Therefore, it is particularly important to develop analytical techniques for the rapid and accurate detection of Fe2+ ions. However, the development of near-infrared (NIR) luminescence probes with good photostability for Fe2+ ions remain challenging. In this work, we report a novel iridium(III) complex-based luminescence probe for the sensitive and rapid detection of Fe2+ ions in a solution based on an Fe2+-mediated reduction reaction. This probe is capable of sensitively detecting Fe2+ ions with a limit of detection (LOD) of 0.26 μM. Furthermore, this probe shows high photostability, and its luminescence remains stable under 365 nm irradiation over a time period of 30 min. To our knowledge, this is first iridium(III) complex-based NIR probe for the detection of Fe2+ ions. We believe that this work provides a new method for the detection of Fe2+ ions and has great potential for future applications in water quality testing and human monitoring. Full article
(This article belongs to the Special Issue Probes for Biosensing and Bioimaging)
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Review

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33 pages, 4616 KiB  
Review
Progression in Near-Infrared Fluorescence Imaging Technology for Lung Cancer Management
by Xinglong Chen, Yuning Li, Jialin Su, Lemeng Zhang and Hongwen Liu
Biosensors 2024, 14(10), 501; https://doi.org/10.3390/bios14100501 - 14 Oct 2024
Cited by 2 | Viewed by 2031
Abstract
Lung cancer is a major threat to human health and a leading cause of death. Accurate localization of tumors in vivo is crucial for subsequent treatment. In recent years, fluorescent imaging technology has become a focal point in tumor diagnosis and treatment due [...] Read more.
Lung cancer is a major threat to human health and a leading cause of death. Accurate localization of tumors in vivo is crucial for subsequent treatment. In recent years, fluorescent imaging technology has become a focal point in tumor diagnosis and treatment due to its high sensitivity, strong selectivity, non-invasiveness, and multifunctionality. Molecular probes-based fluorescent imaging not only enables real-time in vivo imaging through fluorescence signals but also integrates therapeutic functions, drug screening, and efficacy monitoring to facilitate comprehensive diagnosis and treatment. Among them, near-infrared (NIR) fluorescence imaging is particularly prominent due to its improved in vivo imaging effect. This trend toward multifunctionality is a significant aspect of the future advancement of fluorescent imaging technology. In the past years, great progress has been made in the field of NIR fluorescence imaging for lung cancer management, as well as the emergence of new problems and challenges. This paper generally summarizes the application of NIR fluorescence imaging technology in these areas in the past five years, including the design, detection principles, and clinical applications, with the aim of advancing more efficient NIR fluorescence imaging technologies to enhance the accuracy of tumor diagnosis and treatment. Full article
(This article belongs to the Special Issue Probes for Biosensing and Bioimaging)
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34 pages, 10252 KiB  
Review
Fluorescent Probes for Mammalian Thioredoxin Reductase: Mechanistic Analysis, Construction Strategies, and Future Perspectives
by Zilong Song, Chengwu Fan, Jintao Zhao, Lei Wang, Dongzhu Duan, Tong Shen and Xinming Li
Biosensors 2023, 13(8), 811; https://doi.org/10.3390/bios13080811 - 13 Aug 2023
Cited by 3 | Viewed by 2367
Abstract
The modulation of numerous signaling pathways is orchestrated by redox regulation of cellular environments. Maintaining dynamic redox homeostasis is of utmost importance for human health, given the common occurrence of altered redox status in various pathological conditions. The cardinal component of the thioredoxin [...] Read more.
The modulation of numerous signaling pathways is orchestrated by redox regulation of cellular environments. Maintaining dynamic redox homeostasis is of utmost importance for human health, given the common occurrence of altered redox status in various pathological conditions. The cardinal component of the thioredoxin system, mammalian thioredoxin reductase (TrxR) plays a vital role in supporting various physiological functions; however, its malfunction, disrupting redox balance, is intimately associated with the pathogenesis of multiple diseases. Accordingly, the dynamic monitoring of TrxR of live organisms represents a powerful direction to facilitate the comprehensive understanding and exploration of the profound significance of redox biology in cellular processes. A number of classic assays have been developed for the determination of TrxR activity in biological samples, yet their application is constrained when exploring the real-time dynamics of TrxR activity in live organisms. Fluorescent probes offer several advantages for in situ imaging and the quantification of biological targets, such as non-destructiveness, real-time analysis, and high spatiotemporal resolution. These benefits facilitate the transition from a poise to a flux understanding of cellular targets, further advancing scientific studies in related fields. This review aims to introduce the progress in the development and application of TrxR fluorescent probes in the past years, and it mainly focuses on analyzing their reaction mechanisms, construction strategies, and potential drawbacks. Finally, this study discusses the critical challenges and issues encountered during the development of selective TrxR probes and proposes future directions for their advancement. We anticipate the comprehensive analysis of the present TrxR probes will offer some glitters of enlightenment, and we also expect that this review may shed light on the design and development of novel TrxR probes. Full article
(This article belongs to the Special Issue Probes for Biosensing and Bioimaging)
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