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Advances in Phytochemicals: Extraction, Isolation, and Identification

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Chemical and Molecular Sciences".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 570

Special Issue Editor


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Guest Editor
Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Chemical Engineering and Biotechnology, National University of Science and Technology POLITEHNICA Bucharest, Bucharest, Romania
Interests: food; toxicology; natural products; phytochemistry; bioactive substances; antioxidants; volatile oils; chromatography; mass spectrometry; UV–Vis spectroscopy; infrared spectroscopy; environmental chemistry
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Special Issue Information

Dear Colleagues,

This Special Issue is devoted to the most recent scientific advances regarding the extraction, isolation, and identification of phytochemicals. Phytochemicals are natural compounds of plant origin and comprise very diverse chemical classes, including carotenoids, polyphenols, alkaloids, terpenes and terpenoids, phytosterols, saponins, glucosinolates, anthocyanins, tannins, flavonoids, stilbenoids, polyacetylenes, and cuticular waxes, among others. Many of these have important biological activities not only in plants, where they serve as either primary or secondary metabolites, but also as part of human-targeted pharmaceuticals, nutraceuticals, or dietary supplements, e.g., atropine, quercetine, berberine, bromelain, curcumin, cannabidiol derivatives, etc. The separation and isolation of such valuable compounds from plant material are typically carried out by using extractive methods, which can be classical (solvent-based) or modern (ultrasound, microwave, supercritical fluid-based), and are often followed by sequential purification operations usually involving one or several versions of chromatography (CC, TLC, HPLC, and/or GC). After purification, the phytochemical is identified based on either the available literature, or, if novel, its structure is elucidated using an array of analytical methods, amongst which are NMR and mass spectrometry, UV–Vis, IR, and Raman spectroscopy, X-ray crystallography, and circular dichroism in cases where chiral compounds are expected. We welcome original research articles, literature reviews, and short communications on any recent developments in phytochemical research, such as those mentioned above, but not exclusively limited to them.

Yours sincerely,

Dr. Radu Racovita
Guest Editor

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Keywords

  • plant natural products
  • extraction methods
  • isolation and purification
  • structure elucidation
  • nuclear magnetic resonance
  • mass spectrometry
  • circular dichroism
  • X-ray crystallography
  • spectroscopy
  • chromatography

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Published Papers (1 paper)

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Research

13 pages, 2264 KiB  
Article
Biflavonoid Profiling of Juniperus Species: The Influence of Plant Part and Growing Location
by Barbara Medvedec, Iva Jurčević Šangut, Armin Macanović, Erna Karalija and Dunja Šamec
Appl. Sci. 2025, 15(13), 7082; https://doi.org/10.3390/app15137082 - 24 Jun 2025
Viewed by 320
Abstract
Biflavonoids are an important group of flavonoids found in Juniperus species, yet their distribution and accumulation patterns remain insufficiently explored. In this study, we applied a method for the simultaneous quantification of seven biflavonoids to analyze different plant parts of J. communis, [...] Read more.
Biflavonoids are an important group of flavonoids found in Juniperus species, yet their distribution and accumulation patterns remain insufficiently explored. In this study, we applied a method for the simultaneous quantification of seven biflavonoids to analyze different plant parts of J. communis, J. communis subsp. nana, and J. oxycedrus. In order to determinate the influence of growing location, we also analyzed J. communis samples collected from different locations. Four biflavonoids—cupressuflavone, amentoflavone, bilobetin, and hinokiflavone—were detected. In both analyzed J. communis varieties, amentoflavone was the predominant biflavonoid in cones and needles, while in J. oxycedrus, cupressuflavone was the most abundant in cones, with amentoflavone dominating in needles. Overall, biflavonoid content was significantly higher in needles than in cones, with total biflavonoid levels in needles exceeding 5 mg/g dw, highlighting the tissue-specific nature of biflavonoid biosynthesis within Juniperus species. Additionally, our results suggest that in J. communis, biflavonoid accumulation is significantly influenced by growing location. Full article
(This article belongs to the Special Issue Advances in Phytochemicals: Extraction, Isolation, and Identification)
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