State-of-the-Art in Super-Resolution Optical Microscopy

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Optics and Lasers".

Deadline for manuscript submissions: closed (10 August 2022) | Viewed by 7064

Special Issue Editors


E-Mail Website
Guest Editor
1. Molecular Microscopy and Spectroscopy, Istituto Italiano di Tecnologia, Genoa, Italy;
2. Nanoscopy & NIC@IIT, Istituto Italiano di Tecnologia, Genoa, Italy;
3. Genoa Instruments s.r.l.i., Genoa, Italy
Interests: super-resolution optical microscopy; image scanning microscopy; confocal microscopy; fluorescence microscopy; microscopy architectures

E-Mail Website
Guest Editor
Molecular Microscopy and Spectroscopy, Istituto Italiano di Tecnologia, Genoa, Italy
Interests: super-resolution optical microscopy; real-time controlling systems; image analysis; neuronal signaling; smart microscopy architectures

Special Issue Information

Dear Colleagues,

Super-resolution optical microscopy techniques stand as an invaluable tool for the life sciences, allowing the selective observation of diverse structures and processes of interest with diffraction-unlimited spatial resolution, including also in living cells and tissues. These approaches exploit physical or chemical strategies to eventually achieve resolutions down to the single-digit nanometer range, thus providing critical insights for a variety of biological questions.

In the last decades, the scientific community has proposed many strategies to overcome the diffraction barrier, each performing differently in critical aspects such as the maximum achievable spatial and temporal resolution, invasiveness, dependency on post-processing analysis, and compatibility with time-resolved assays. In such a broad and exciting scenario, it is often possible to find the technique characterized by the performance fingerprint that best suits the experimental needs.

Within this framework, this Special Issue aims to cover advancements in the field of super-resolution optical microscopy by presenting novel techniques, improvements in technological implementations, new analysis tools to increase the information output, and critical applications.

Dr. Marco Castello
Dr. Giorgio Tortarolo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • super-resolution
  • optical microscopy
  • nanoscopy
  • fluorescence microscopy
  • image analysis
  • imaging

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

Jump to: Review

12 pages, 4059 KiB  
Article
Image Scanning Microscopy to Investigate Polycomb Protein Colocalization onto Chromatin
by Irene Nepita, Simonluca Piazza, Martina Ruglioni, Sofia Cristiani, Emanuele Bosurgi, Tiziano Salvadori, Giuseppe Vicidomini, Alberto Diaspro, Marco Castello, Paolo Bianchini, Barbara Storti and Ranieri Bizzarri
Appl. Sci. 2023, 13(3), 1556; https://doi.org/10.3390/app13031556 - 25 Jan 2023
Cited by 3 | Viewed by 1818
Abstract
Super-resolution microscopy has been recently applied to understand the 3D topology of chromatin at an intermediated genomic scale (kilobases to a few megabases), as this corresponds to a sub-diffraction spatial scale crucial for the regulation of gene transcription. In this context, polycomb proteins [...] Read more.
Super-resolution microscopy has been recently applied to understand the 3D topology of chromatin at an intermediated genomic scale (kilobases to a few megabases), as this corresponds to a sub-diffraction spatial scale crucial for the regulation of gene transcription. In this context, polycomb proteins are very renowned gene repressors that organize into the multiprotein complexes Polycomb Repressor Complex 1 (PRC1) and 2 (PRC2). PRC1 and PRC2 operate onto the chromatin according to a complex mechanism, which was recently recapitulated into a working model. Here, we present a functional colocalization study at 100–140 nm spatial resolution targeting PRC1 and PRC2 as well as the histone mark H3K27me3 by Image Scanning Microscopy (ISM). ISM offers a more flexible alternative to diffraction-unlimited SRMs such as STORM and STED, and it is perfectly suited to investigate the mesoscale of PRC assembly. Our data suggest a partially simultaneous effort of PRC1 and PRC2 in locally shaping the chromatin topology. Full article
(This article belongs to the Special Issue State-of-the-Art in Super-Resolution Optical Microscopy)
Show Figures

Figure 1

Review

Jump to: Research

20 pages, 370 KiB  
Review
The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy
by Colin J. R. Sheppard
Appl. Sci. 2021, 11(19), 8981; https://doi.org/10.3390/app11198981 - 27 Sep 2021
Cited by 13 | Viewed by 4336
Abstract
Optical methods of super-resolution microscopy, such as confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These methods avoid strong invasive interaction with a sample, allowing the observation of delicate biological samples. The meaning of resolution and the basic principles [...] Read more.
Optical methods of super-resolution microscopy, such as confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These methods avoid strong invasive interaction with a sample, allowing the observation of delicate biological samples. The meaning of resolution and the basic principles and different approaches to superresolution are discussed. Full article
(This article belongs to the Special Issue State-of-the-Art in Super-Resolution Optical Microscopy)
Show Figures

Figure 1

Back to TopTop