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Microorganisms and Their Use in Biotechnological Production, 2nd Edition

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Microbiology".

Deadline for manuscript submissions: 20 June 2026 | Viewed by 866

Special Issue Editors


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Guest Editor
Institute of Biology, University of Graz, Universitätsplatz 2, 8010 Graz, Austria
Interests: environmentally sustainable biotechnology; bioremediation; microbial chemistry; photosynthetic microorganisms; methylotrophic yeasts; heterotrophic bacteria
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Special Issue Information

Dear Colleagues,

Microorganisms play a pivotal role in biotechnological processes, shaping industries across diverse domains. From pharmaceuticals to environmental remediation, their versatility is unmatched.

Microorganisms have been used in different applications for thousands of years, especially in foods such as bread or in beer production. In recent decades, their uses have increased in chemical and biofuel production. Especially in the case of phototrophic microorganisms, their capability to capture CO2 and utilize light has been exploited to produce different compounds. Furthermore, the applications of yeasts and bacteria in waste treatment, bioremediation of environmental pollutants, or as ameliorants against biotic and abiotic stressors have also increased. However, we still require knowledge regarding their mechanisms of action during their applications and regarding the potential use of new microorganisms.

This Special Issue aims to explore the intricate interplay between microorganisms and biotechnological production. We invite contributions that delve into microbial physiology, metabolic engineering, fermentation, and novel applications, among others.

By fostering interdisciplinary dialogue, we aspire to unravel new frontiers in biotechnology.

We look forward to your contributions.

Prof. Dr. Dirk Tischler
Dr. Giovanni Barone
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • environmentally sustainable biotechnology
  • industrial and agro-environmental biotechnologies
  • microbial metabolites and bioprocessing
  • yeasts, bacteria, and microalgae
  • genetic and metabolic engineering

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Published Papers (1 paper)

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Research

17 pages, 2421 KB  
Article
Physicochemical Determinants of Storage Stability in Spore-Based Bacterial Biopreparations for Agricultural Use
by Patrycja Rowińska, Beata Gutarowska, Marta Wasilewska, Szymon Powałowski and Justyna Szulc
Appl. Sci. 2025, 15(22), 11856; https://doi.org/10.3390/app152211856 - 7 Nov 2025
Viewed by 677
Abstract
The stability of spore-forming soil bacteria is crucial for their effective use in agricultural biopreparations. This study evaluated the long-term survivability of selected strains (Paenibacillus amylolyticus, Priestia megaterium, Bacillus velezensis, Bacillus subtilis, and Bacillus licheniformis) with potential [...] Read more.
The stability of spore-forming soil bacteria is crucial for their effective use in agricultural biopreparations. This study evaluated the long-term survivability of selected strains (Paenibacillus amylolyticus, Priestia megaterium, Bacillus velezensis, Bacillus subtilis, and Bacillus licheniformis) with potential applications in biopreparations for crop residue decomposition. The effects of different storage and preservation conditions on vegetative cells and bacterial spores were studied over 12 months. Bacteria were stored at different temperatures (15 °C, 21 °C, 30 °C), pH levels (5, 9, and post-cultivation liquid pH), and osmotic pressures (2%, 5%, and 10% of carbamide, calcium chloride, and multicomponent fertilizer). Additionally, freeze-drying, spray-drying and freezing were performed using cryoprotectants (skimmed milk, trehalose, and glycerol). The results showed that bacterial stability depended on both the strain and storage conditions. Vegetative cells of P. amylolyticus and B. velezensis were most sensitive to temperatures of 30 °C, whereas the spores of most strains demonstrated high temperature resistance. The tested strains exhibited better survivability at pH 5 than pH 9. The addition of calcium chloride, carbamide, or multicomponent fertilizer proved beneficial for maintaining viability, especially increasing spore numbers. Trehalose and skimmed milk were the most effective cryoprotectants overall, though efficacy varied by strain and cell form. These findings provide insight into the optimal conditions for preserving the bacterial viability of spore-producing bacteria in bioformulations, which is crucial for maintaining their effectiveness in agricultural applications. Full article
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