Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
5.5
2.5
Journals
Applied Sciences
Special Issues
Modern Trends and Applications in Cell Imaging
applsci-logo
Submit to Special Issue Submit Abstract to Special Issue Review for Applied Sciences Propose a Special Issue

Journal Menu

► Journal Menu

Journal Browser

► Journal Browser
share announcement

Modern Trends and Applications in Cell Imaging

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 711

Special Issue Editors

Dr. Patrizio Candeloro

E-Mail Website
Guest Editor
BIONEM Lab., Department of experimental and clinical medicine, “Magna Graecia” University of Catanzaro, 88100 Catanzaro, Italy
Interests: plasmonics; optical nanodevices; micro- and nanofabrication; spectroscopy and their applications on biological and medical fields
Special Issues, Collections and Topics in MDPI journals
Dr. Luca Tirinato

E-Mail Website
Guest Editor
Department of Medical and Surgical Sciences, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, Italy
Interests: biophotonics; lipids

Special Issue Information

Dear Colleagues,

Cell imaging has revolutionized the field of biomedical research, enabling scientists to visualize cellular structures, track dynamic processes, and gain deeper insights into disease mechanisms. Modern trends in cell imaging are focusing on enhancing resolution, speed, and specificity while minimizing phototoxicity. Advancements in fluorescence, hyperspectral imaging, and label-free imaging are improving the real-time observation of living cells, enabling the more precise spectral analysis of cellular components. Meanwhile, super-resolution microscopy techniques, such as STED, allow imaging beyond the diffraction limit, revealing nanoscale cellular details.

Artificial intelligence (AI) and machine learning are transforming image analysis, automating feature recognition and quantification. Multiplex imaging techniques, including spatial transcriptomics, enable the high-dimensional analysis of cellular interactions within tissues. Additionally, innovations in miniaturized and portable imaging devices are expanding diagnostic applications in clinical and point-of-care settings.

Applications of modern cell imaging span various domains, from cancer research and drug discovery to neuroscience and regenerative medicine. Live-cell imaging aids in understanding cellular dynamics, while high-throughput imaging accelerates screening for potential therapeutics. With continuous technological advancements, cell imaging is set to further enhance precision medicine and personalized treatment strategies, making it an indispensable tool in modern life sciences and healthcare.

Dr. Patrizio Candeloro
Dr. Luca Tirinato
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • high-resolution imaging of cells
  • advancements in fluorescence imaging of cells
  • stimulated emission depletion (STED) microscopy in cells
  • hyperspectral imaging of cells
  • Raman micro-imaging for cells
  • artificial intelligence (AI) applications in cell imaging
  • machine learning applications in cell imaging

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (1 paper)

Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

14 pages, 5396 KB  
Article
Hypoxia-Induced Extracellular Matrix Deposition in Human Mesenchymal Stem Cells: Insights from Atomic Force, Scanning Electron, and Confocal Laser Microscopy
by Agata Nowak-Stępniowska, Paulina Natalia Osuchowska, Henryk Fiedorowicz and Elżbieta Anna Trafny
Appl. Sci. 2025, 15(19), 10701; https://doi.org/10.3390/app151910701 - 3 Oct 2025
Viewed by 513
Abstract
(1) Background: The extracellular matrix (ECM) is a natural scaffold for cells, creating a three-dimensional architecture composed of fibrous proteins (mainly collagen) and proteoglycans, which are synthesized by resident cells. In this study, a physiological hypoxic environment was utilized to enhance ECM production [...] Read more.
(1) Background: The extracellular matrix (ECM) is a natural scaffold for cells, creating a three-dimensional architecture composed of fibrous proteins (mainly collagen) and proteoglycans, which are synthesized by resident cells. In this study, a physiological hypoxic environment was utilized to enhance ECM production by human mesenchymal stem cells (hMSCs), a process relevant to tissue engineering and regenerative medicine. (2) Methods: hMSCs were treated with deferoxamine (DFO), a pharmaceutical hypoxia-mimetic agent that induces cellular responses similar to low-oxygen conditions through stabilization of hypoxia inducible factor-1α (HIF-1α). The time points 0 h 24 h, 3 h 24 h, and 24 h 24 h refer to DFO being added immediately after cell seeding (before cells adhesion), 3 h after cell seeding (during initial cells attachment), and 24 h after cell seeding (after focal adhesions formation and actin organization), respectively, to evaluate the influence of cell adhesion on ECM deposition. hMSCs incubated in culture media were subsequently exposed to DFO for 24 h. Samples were then subjected to cell viability tests, scanning electron microscopy (SEM), atomic force microscopy (AFM) and laser scanning confocal microscopy (CLSM) assessments. (3) Results: Viability tests indicated that DFO concentrations in the range of 0–300 µM were non-toxic over 24 h. The presence of collagen fibers in the DFO-derived ECM was confirmed with anti-collagen antibodies under CLSM. Increased ECM secretion was observed under the following conditions: 3 μM DFO (24 h 24 h), 100 μM DFO (0 h 24 h) and 300 μM DFO (3 h 24 h). SEM and AFM images revealed the morphology of various stages of collagen formation with both collagen fibrils and fibers identified. (4) Conclusions: Our preliminary study demonstrated enhanced ECM secretion by hMSC treated with DFO at concentrations of 3, 100, and 300 µM within a short cultivation period of 24–48 h without significant affecting cell viability. By mimicking physiological processes, it may be possible to stimulate endogenous tissue regeneration, for example, at an injury site. Full article
(This article belongs to the Special Issue Modern Trends and Applications in Cell Imaging)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-1
Back to TopTop