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Bioengineering Microbial Control: Precision Tools for Sustainable Agriculture

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: 20 May 2026 | Viewed by 577

Special Issue Editor

Special Issue Information

Dear Colleagues,

The integration of bioengineering and microbial control in agriculture represents a cutting-edge approach to achieving sustainable farming practices. By utilizing genetically engineered microbes, beneficial microbial consortia, and precise biocontrol agents, crops can be protected from pathogens, pests, and environmental stresses while minimizing chemical inputs. Precision tools such as CRISPR-based gene editing, synthetic biology, and microbiome engineering allow for targeted manipulation of plant–microbe interactions, improving nutrient uptake, soil health, and crop productivity. Additionally, these strategies support climate-resilient agriculture by enhancing plant tolerance to drought, salinity, and other abiotic stresses. The adoption of bioengineered microbial solutions not only reduces ecological footprints but also contributes to resource-efficient farming, biodiversity preservation, and long-term food security, making it a key component of next-generation sustainable agriculture.

Dr. Spiridon Mantzoukas
Guest Editor

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Keywords

  • bioengineering
  • microbial control
  • precision agriculture
  • sustainable farming
  • biocontrol agents
  • CRISPR
  • synthetic biology
  • plant–-microbe interactions
  • crop resilience
  • eco-friendly agriculture

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Published Papers (1 paper)

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Research

15 pages, 1164 KB  
Article
Long-Term Field Efficacy of Entomopathogenic Fungi Against Tetranychus urticae: Host Plant- and Stage-Specific Responses
by Spiridon Mantzoukas, Chrysanthi Zarmakoupi, Vasileios Papantzikos, Thomais Sourouni, Panagiotis A. Eliopoulos and George Patakioutas
Appl. Sci. 2026, 16(2), 1109; https://doi.org/10.3390/app16021109 - 21 Jan 2026
Viewed by 400
Abstract
The two-spotted spider mite, Tetranychus urticae Koch, is a major agricultural pest whose control is increasingly constrained by resistance to synthetic acaricides. This study evaluated the long-term field efficacy of three commercial entomopathogenic fungal (EPF) biopesticides—Velifer® (Beauveria bassiana), Metab® [...] Read more.
The two-spotted spider mite, Tetranychus urticae Koch, is a major agricultural pest whose control is increasingly constrained by resistance to synthetic acaricides. This study evaluated the long-term field efficacy of three commercial entomopathogenic fungal (EPF) biopesticides—Velifer® (Beauveria bassiana), Metab® (B. bassiana + Metarhizium anisopliae), and Botanigard® (B. bassiana)—against larval and protonymph stages of T. urticae on two host plants, Italian cypress (Cupressus sempervirens) and sweet orange (Citrus sinensis). Two foliar applications were conducted during the 2023 growing season (25 May and 25 July), and mite populations were monitored for 140 days after the final application. A randomized complete block design was used, and efficacy was calculated using the Henderson–Tilton formula. All EPF treatments significantly reduced mite populations compared with the untreated control throughout the monitoring period. Velifer consistently achieved the highest suppression of larval populations, particularly on C. sinensis, with efficacy comparable to the chemical standard. Botanigard showed more gradual but sustained population reduction over time, whereas Metab exhibited lower but stable efficacy in all cases. Treatment performance was strongly influenced by host plant species and mite developmental stage, with larvae consistently more susceptible than protonymphs. On C. sinensis, Velifer achieved the highest larval suppression (84.6%), comparable to the chemical standard abamectin, while Botanigard and Velifer were most effective on C. sempervirens. Survival analysis confirmed isolate- and host-dependent differences in hazard effects over time. These results demonstrate that EPF-based products can provide sustained, long-term suppression of T. urticae under field conditions, supporting their integration into integrated pest management programs. Full article
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