Bacteriophage Therapy: A Renaissance Weapon—Recent Developments and Application, 3rd Edition

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "Bacteriophages".

Deadline for manuscript submissions: 31 August 2026 | Viewed by 778

Special Issue Editors


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Guest Editor
Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, Poland
Interests: application of bacteriophages in human and veterinary medicine; development of the methods of working with phages; interactions of bacteriophages with bacterial and non-bacterial cells
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Guest Editor
Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland
Interests: bacteriophages
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Over the past century, antibiotic therapy has become the most powerful tool for combating bacterial infections. However, the era of their universal effectiveness appears to be drawing to a close. The number of new antibiotics being entering the market has steadily declined, and pharmaceutical companies have become increasingly reluctant to pursue their development due to the high risk of failure. This diminishing pharmacological treatment of bacterial infections is accompanied by an increasing number of antibiotic-resistant—and, more broadly, drug-resistant bacterial strains. Consequently, the inevitable need to identify and implement alternative strategies to combat bacterial infections has become increasingly urgent.

Bacteriophages have been used to treat bacterial infections almost since their discovery at the beginning of the 20th century. Nevertheless, with the advent and widespread success of antibiotics, phage therapy was marginalized for decades. Today, in the face of the rapidly growing number of drug-resistant bacterial strains, phage therapy re-emerged as one of the most promising alternatives to conventional methods of treatment. Moreover, research on bacteriophages now extends beyond human and veterinary medicine, increasingly reaching new fields of biotechnology.

Dr. Aneta Skaradzińska
Dr. Paulina Śliwka
Guest Editors

Manuscript Submission Information

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Keywords

  • bacteriophages
  • phage therapy
  • application of phages
  • antibiotic-resistance
  • antibacterial therapies

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Research

18 pages, 6494 KB  
Article
Toxinotyping, Antibiotic Resistance Profile, and In Vitro Bio-Control of Clostridium perfringens Type G Isolated from Chickens with Necrotic Enteritis by Lytic Bacteriophages
by Hoang Minh Duc, Nguyen Thi Lan, Tran Thi Khanh Hoa, Cam Thi Thu Ha, Le Van Hung, Nguyen Van Thang and Hoang Minh Son
Antibiotics 2026, 15(5), 453; https://doi.org/10.3390/antibiotics15050453 - 30 Apr 2026
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Abstract
Background/Objectives: Necrotic enteritis (NE), induced by Clostridium perfringens, is responsible for significant economic losses in the poultry industry worldwide. The growing restrictions on antibiotic use have driven the search for alternative strategies for disease control. The purpose of this study is to [...] Read more.
Background/Objectives: Necrotic enteritis (NE), induced by Clostridium perfringens, is responsible for significant economic losses in the poultry industry worldwide. The growing restrictions on antibiotic use have driven the search for alternative strategies for disease control. The purpose of this study is to isolate and characterize lytic phages targeting multidrug-resistant C. perfringens type G recovered from chickens with NE. Methods: C. perfringens was isolated from chickens with NE using a culture method with selective TSC agar. Bacterial identification was carried out using biochemical tests and PCR. C. perfringens isolates were toxinotyped by PCR. Antibiotic susceptibility test was performed using the agar dilution method. Bacteriophages were isolated from chicken intestine samples collected from wet markets using the double-layer agar technique. Phage isolates were characterized by host range, one-step growth, stability, and whole genome sequencing. The efficacy of phage CPP8 in controlling multidrug-resistant C. perfringens type G was evaluated in GAM broth. Results: In this study, 16 C. perfringens strains were isolated from 100 chickens suspected of NE. Among these isolates, 10 (62.5%) belonged to type G, while the remaining 6 (37.5%) were type A. A total of 11 phages capable of lysing C. perfringens type G were isolated from the chicken intestine. Among them, phage CPP8 has the widest host range, short latent period, large burst size, and high stability. Moreover, the genome of CPP8 lacked genes related to antibiotic resistance, toxins, virulence factors, or lysogeny. Treatment with CPP8 resulted in a significant reduction in viable counts of C. perfringens at 37 °C. Conclusions: Our findings highlight phage CPP8 as a promising candidate for bio-control of multidrug-resistant C. perfringens type G. Full article
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