Diagnostic Strategies in Bovine Respiratory Disease: 2nd Edition

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Cattle".

Deadline for manuscript submissions: 28 February 2026 | Viewed by 456

Special Issue Editor


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Guest Editor
Laboratory of Animal Pathology, Veterinary Hospital, Department of Veterinary Preventive Medicine, ‎Universidade Estadual de Londrina, Campus Universitário, Londrina, Brazil
Interests: bovine respiratory disease; diagnostic immunohistochemistry; histological patterns of infectious disease agents; malignant catarrhal fever; molecular epidemiology; pathogenesis
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Special Issue Information

Dear Colleagues,

Bovine respiratory disease (BRD) complex causes serious losses in terms of morbidity, mortality, and the related therapy of affected animals worldwide. The infectious disease agents associated with the development of BRD continue to develop as new diagnostic strategies are implemented or conventional techniques methods are modified. Additionally, improved diagnosis remains a principal aim in preventing loss due to productivity, mortality, and morbidity, complementing the understanding of the pathogenesis of infectious BRD agents and defining the participation of singular and/or concomitant agents in the participation of pulmonary disease in dairy and feedlot cattle.

Following the success of the initial edition, we are pleased to invite your contributions to the second phase of this project, which focuses on all diagnostic methods related to the characterization of infectious disease agents and/or the clinical diagnosis of bovine respiratory disease (BRD). We welcome articles exploring scientific methods for diagnosis, such as clinical forms of laboratory diagnosis including, but not limited to, histopathology, bacteriology, immunohistochemistry, in situ hybridization, serology, molecular, respiratory microbiota, and new-generation sequencing. 

Original research articles and reviews are welcome in this Special Issue.

Prof. Dr. Selwyn Arlington Headley
Guest Editor

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Keywords

  • research areas may include (but are not limited to) the following: clinical diagnosis and studies
  • serological epidemiology
  • histopathological, immunohistochemistry, and in situ hybridization
  • molecular characterization
  • bacteriological and virological identification
  • new-generation sequencing
  • respiratory microbiota of cattle
  • meta-analyses and/or reviews

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Published Papers (1 paper)

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20 pages, 6486 KB  
Case Report
Outbreak of Respiratory Disease Due to Bovine Respiratory Syncytial Virus with Concomitant Infections by Histophilus somni and Pasteurella multocida in Adult Dairy Cows and Calves from Southern Brazil
by João Henrique Perotta, Isabela Vaz Silva, Maria Constanza Rodriguez, Mara Eliza Gasino Joineau, Marcel Kruchelski Tschá, Renato Silva de Sousa, Alais Maria Dall Agnol, Flávia Helena Pereira Silva, Sébastien Buczinski, Selwyn Arlington Headley and Ivan Roque de Barros Filho
Animals 2025, 15(20), 3015; https://doi.org/10.3390/ani15203015 - 17 Oct 2025
Viewed by 298
Abstract
Although bovine respiratory syncytial virus (BRSV) is a key contributor to bovine respiratory disease (BRD) worldwide, there are few detailed reports of BRSV-related outbreaks in Brazil. This study describes the clinical, pathological, immunohistochemical (IHC), and molecular findings from a BRD outbreak in adult [...] Read more.
Although bovine respiratory syncytial virus (BRSV) is a key contributor to bovine respiratory disease (BRD) worldwide, there are few detailed reports of BRSV-related outbreaks in Brazil. This study describes the clinical, pathological, immunohistochemical (IHC), and molecular findings from a BRD outbreak in adult dairy cows from Southern Brazil. The affected cattle had dyspnea, nasal discharge, and coughing. One cow died, and samples were collected for diagnosis. Histopathology revealed interstitial pneumonia with multinucleated giant cells. IHC identified BRSV antigens in pulmonary tissue. A multiplex real-time PCR identified BRSV, Histophilus somni, and Pasteurella multocida in nasal and oral swabs, while only BRSV and H. somni were detected in the tissues of the cow that died. All animals had co-infections involving BRSV. The average cycle threshold (Cq) values for BRSV were 27.43 (nasal) and 32.68 (oral), with significant differences (p = 0.016), indicating higher nasal shedding. This qPCR assay was effective for detecting BRD pathogens, the quantification of viral and bacterial loads in animals with BRD and can be used for the rapid detection of respiratory pathogens. The elevated BRSV detection in oral samples suggests that this route may be an alternative for the collection of samples in cattle with profuse nasal discharge. Full article
(This article belongs to the Special Issue Diagnostic Strategies in Bovine Respiratory Disease: 2nd Edition)
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