Feature Papers in Veterinary Clinical Studies: From Current to Emerging Technologies and Innovative Approaches for the Future

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Veterinary Clinical Studies".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 371

Special Issue Editors


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Guest Editor
Department of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, Italy
Interests: canine and feline tumors; bovine tumors

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Guest Editor
Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy
Interests: swine pathology; tumors in domestic carnivores; genital pathology

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Guest Editor
Department of Veterinary Medicine, University of Teramo, Teramo, Italy
Interests: tumors in domestic mammals; heat shock proteins; oncoproteins
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, Italy
Interests: cancer cytogenetics; mammary tumors; veterinary pathology; animal oncology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Veterinary medicine encompasses many seemingly diverse yet interconnected fields. These range from forensic medicine, which focuses on examining injuries resulting from animal cruelty and known or emerging diseases, to investigating neoplasms, as well as studying fish and insect indicators of environmental pollutants. Increasingly sophisticated diagnostic methods can detect, with utmost precision and speed, pathologies in both domestic and wild species, from mammals to fish to insects, based on alterations in organs and systems, from tissues to the cells that constitute them.

Advanced tools are increasingly used to improve the accuracy and expediency of zoonotic disease diagnosis and epidemiology. Intersectoral surveillance entails monitoring animal and human populations, as well as their environments, to identify potential threats and transmission patterns.

This Special Issue includes techniques such as metabolomics, the study of cell culture arrays, scanning electron microscopy, next-generation sequencing, advanced immunohistochemistry techniques, forensic pathology, etc., exemplifying scientific progression for the future of veterinary medicine.

Original contributions and reviews dealing with the above-mentioned topics are welcome.

Prof. Dr. Cinzia Benazzi
Prof. Dr. Giuseppe Sarli
Prof. Dr. Leonardo Della Salda
Dr. Luisa Vera Muscatello
Guest Editors

Manuscript Submission Information

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Keywords

  • gold standard diagnostic techniques
  • pathology of domestic and wild animals
  • fish
  • zoonosis
  • environmental pollutants

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Published Papers (1 paper)

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Research

13 pages, 1842 KiB  
Article
Bright-Field Multiplex Immunohistochemistry in Swine PCV2 and PRRSV Lymphadenopathies
by Giulia D’Annunzio, Luisa Vera Muscatello, Chiara Tugnoli, Stefano Pesaro, Andrea Luppi, Michelangelo Fiorentino, Tania Franceschini, Alessia Grillini, Gianluca Rugna, Giuseppe Sarli and Luciana Mandrioli
Animals 2025, 15(12), 1682; https://doi.org/10.3390/ani15121682 - 6 Jun 2025
Viewed by 219
Abstract
Multiplex immunostaining (mIHC) allows the simultaneous detection of multiple antigenic targets within the same tissue section, providing a deeper understanding of spatial variation in cellular distribution. The aim of the present study is to apply this technique to examine the spatial variation of [...] Read more.
Multiplex immunostaining (mIHC) allows the simultaneous detection of multiple antigenic targets within the same tissue section, providing a deeper understanding of spatial variation in cellular distribution. The aim of the present study is to apply this technique to examine the spatial variation of lymphocyte populations in swine lymph nodes during PCV2-SD and PRRSV lymphadenopathy compared with reactive lymphoid hyperplasia. A triple immunohistochemical stain with CD3, CD20 and IBA1 antibodies for the concurrent detection of T lymphocytes, B lymphocytes and macrophages, respectively, was performed. Multiplex immunohistochemistry (mIHC) revealed that, compared to reactive hyperplasia, the most significant changes in lymph node cell populations occurred in the follicles for both PCV2 and PRRSV infections. Additionally, in PCV2 cases, notable alterations were also observed in the interfollicular areas. In PCV2-affected lymph nodes, follicles not only significantly decreased in number but also showed a marked significant reduction in CD20+ and CD3+ cells. The interfollicular region in these cases also exhibited a significant reduction in CD3+ cells. In contrast, PRRSV-associated lymphadenopathy showed significantly increased CD20+ cells in the follicles, with a similar trend noted in the interfollicular region. mIHC provides more informative results on a single tissue section, thus preserving the topographical information of the tissue and allowing a comprehensive study of cellular composition, cellular functionality and cell–cell interactions, proving to be a valuable tool for studying and understanding disease dynamics. Full article
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